20410223 -
BIOLOGIA MOLECOLARE
(objectives)
The course concerns the general understanding of the molecular mechanisms which regulate the biological processes occurring within a cell, with particular emphasys on genetic structure, organization and expression of prokaryotic and eukaryotic organisms. Furthermore, the subject takes into consideration the most updated molecular biology Experimental procedures utilized in basic and applied research.
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CERVELLI MANUELA
( syllabus)
1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.
( reference books)
Recommended textbooks: 1. METODOLOGIE BIOCHIMICHE STRUMENTI E TECNICHE PER IL LABORATORIO DEL NUOVO MILLENNIO, MACCARRONE M., E BIOMOLECOLARI, CASA EDITRICE ZANICHELLI 2. DAI GENI AI GENOMI, JEREMY W. DALE, MALCOLM VON SCHANTZ, NICK PLANT, A CURA DI: E. GINELLI, CASA EDITRICE EDISES 3. ANALISI DEI GENI E GENOMI, REECE, EDISES. 4. TECNICHE E METODI PER LA BIOLOGIA MOLECOLARE, AMALDI F., BENEDETTI P., PESOLE G., PLEVANI P., CASA EDITRICE AMBROSIANA.
Lecture notes and calculation exercises will be provided. Non-attending students are invited to request information on the program, teaching materials and how to evaluate the profit. The professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it
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ANTONINI GIOVANNI
( syllabus)
THE COURSE OF MOLECULAR BIOLOGY IS PART OF THE TEACHING ACTIVITIES OF THE DEGREE COURSE IN LIFE SCIENCES. THE COURSE THROUGH FRONTAL LESSONS AND LABORATORY TRAINING AIMS TO PROVIDE THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. TOOLS WILL BE GIVEN TO UNDERSTAND THE MAIN MOLECULAR BIOLOGY STRATEGIES AND TECHNIQUES UTILIZED TO STUDY GENES AND GENOMES. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE STUDENTS WILL GET ACQUAINTED WITH THEORETICAL KNOWLEDGES AND METHODOLOGICAL SKILLS THAT WILL ENABLE THEM TO APPLY THE CORRECT STUDY TO SOLVE SPECIFIC PROBLEMS IN THE FIELD OF MOLECULAR BIOLOGY. THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; DNA REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.
( reference books)
BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI BIOLOGIA MOLECOLARE, CAPRANICO ET AL, II EDIZIONE, CASA EDITRICE EDISES FONDAMENTI DI BIOLOGIA MOLECOLARE, L.A. ALLISON, SECONDA EDIZIONE ITALIANA, CASA EDITRICE ZANICHELLI The professor receives every day from 8.00 to 9.00 by appointment via e-mail: giovanni.antonini@uniroma3.it
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6
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BIO/11
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48
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Basic compulsory activities
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3
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BIO/11
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16
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10
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Core compulsory activities
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ITA |
20410225 -
ECOLOGIA
(objectives)
Major goals of this course are the achievement of solid skills in the various sectors of ecology (such as ecosystem, community and population ecology); solid training in basic ecology and applied ecology address to promote the protection, conservation and enhancement of natural resources; the strengthening and application of previously acquired knowledge (such as Botany and Zoology) in an ecological context; field observation and analysis of ecological issues; the development of interest in ecological questions in relation to man and environment; to develop basic elements for the collection, organization and interpretation of ecological data, both in laboratory and in field.
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ACOSTA ALICIA TERESA ROSARIO
( syllabus)
Looking at nature through different perspectives: ecological principles from ecosystems to populations, origin and development of ecology as a science. Ecology as a group of subdisciplines. Ecosystem ecology: factors related to distribution of biomes. The main terrestrial biomes. Biomes and climate change. Ecology of ecosystems. Energy in ecosystems. Thermodynamic aspects of energy flows. Trophic structure. Production and productivity. Primary and secondary production. Methods for measuring primary productivity. Ecological pyramids. Biogeochemical cycles. Main biogeochemical cycles. Changes to biogeochemical cycles related to anthropic activities. Community ecology: Community structure. Community analysis methods. Community dynamics. Ecological successions. Methods for studying succession. Diversity. Meaning and calculation of diversity. The factors that regulate diversity. Diversity in different ecosystems. Threats to biodiversity. Red lists of endangered species. The exotic species. Examples in Italian flora and fauna. Bioindicators. Population ecology. Population structure and dynamics. Selection r and k. Inter-specific relationship interactions. Interspecific competition and ecological niche. Physical environment and organism-environment relations. Ecological factors. Response of organisms to environmental factors. Stress-Disturbance. The C-S-R strategies. Ecosystems conservation and management. Biodiversity Conservation. Introduction to conservation ecology. Protected areas. Introduction to landscape ecology. Landscape structure and landscape dynamics, landscape and ecosystem management. Introduction to urban ecology. Major features of urban ecosystems.
( reference books)
SMITH, T. & SMITH, R. 2017. ELEMENTI DI ECOLOGIA. PEARSON-B. CUMMINGS CAIN, M. BOWMAN, W. & HACKER, S. 2017. ECOLOGIA. PICCIN The material will be indicated by the teacher during the lessons
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SCALICI MASSIMILIANO
( syllabus)
distribution and dispersion; density, structure (genetic and demographic) and population dynamics; time table; interactions between species
( reference books)
Elementi di Ecologia, Thomas M. Smith & Robert L. Smith (Autore). Pearson
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6
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BIO/07
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48
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-
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-
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Basic compulsory activities
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3
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BIO/07
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16
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-
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Core compulsory activities
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ITA |
20410227 -
FISIOLOGIA VEGETALE
(objectives)
Provide a solid and updated knowledge on the molecular and physiological processes underlying the functioning of cells and different organs of plant organisms as well as on the fundamental processes that regulate the life of plants. Provide the tools to develop the ability of critical analysis of experimental results and the ability to solve problems through laboratory didactics. The study of plant physiology assumes a fundamental role in different disciplinary sectors and application fields as for example, the genetic improvement of plants, plant biotechnologies, pharmacology, plant pathology, plant ecology.
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TAVLADORAKI PARASKEVI
( syllabus)
Learning tasks To increase knowledge concerning fundamental processes underlying plant life as well as provide students with basic expertise in experimental plant physiology. Plant Physiology has a primary importance in many scientific areas and applicative contexts such as plant molecular biology, plant breeding, pharmacology, plant pathology, agro-industrial biotechnology.
Program of the lessons -Growth and Development. -Auxin: Generalities and Signal Transduction (polar transport, extension growth, phototropism, gravitropism). -Gibberellines, Cytokinines, Ethylene, Abscissic Acid, Brassinosteroids: Generalities and Signal Transduction. -Photomorphogenesis, Photereceptors and Responses to variations of the spectral quality of the light. -Photoperodism. -Control of Flowering.
Laboratory exercises: 1) Determination of water potential in glycophytes and halophytes, weight gain / loss method; 2) Photosynthesis: preparation of thylakoid vesicles and their use in the Hill reaction; 3) Surface tension and capillarity: physical mathematical derivation of the law ofJurin and realization of a variable radius capillary that highlights the characteristics of a branch of equilateral hyperbole.
( reference books)
PLANT PHYSIOLOGY, Lincoln Taiz and Eduardo Zeiger, editor SINAUER ASSOCIATES (Sixth EDITION; in english language)
FISIOLOGIA VEGETALE, Lincoln Taiz e Eduardo Zeiger, editore PICCIN, QUARTA EDIZIONE ITALIANA (dalla Quinta edizione in lingua Inglese)
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CONA ALESSANDRA
( syllabus)
Learning tasks To increase knowledge concerning fundamental processes underlying plant life as well as provide students with basic expertise in experimental plant physiology. Plant Physiology has a primary importance in many scientific areas and applicative contexts such as plant molecular biology, plant breeding, pharmacology, plant pathology, agro-industrial biotechnology.
Course program The plant cell. The cell wall: structure, biogenesis and role in cell expansion. Water and plant cells: structure and properties of water, water transport processes (diffusion, mass flow, osmosis), water potential. The osmotic behavior of plant cells. Aquaporins. The water balance of the plant and the soil-plant-atmosphere continuum: the absorption of water in the root, xylem transport (Tension-Cohesion theory), transpiration. Stomata: morphology, opening and closing mechanisms, regulation by environmental signals. The transport of solutes in solution and across a membrane barrier: the electrochemical potential and the Nernst equation. The transport of solutes across biological membranes: transport proteins, active and passive transport, membrane potential in plant cells. Photosynthesis: light reactions. General concepts, organization of the photosynthetic apparatus, antenna systems, reaction centers, excitation of photosynthetic pigments, photosynthetic electron transport chain, Z-scheme, redox potential, water photolysis, plastoquinone and cytochrome complex. Proton gradient formation and ATP synthesis in the chloroplast. Regulation of the photosynthetic process under excessive light: photoinhibition and photoprotection of PSII. Photosynthesis: carbon reactions. Calvin-Benson cycle, photorespiration, mechanisms of CO2 concentration in C4 and CAM plants, starch and sucrose biosynthesis, carbohydrate allocation and accumulation. Translocation in the phloem: pressure flow model, loading and unloading. Respiration: glycolysis, pentose phosphate pathway, citric acid cycle, mitochondrial electron transport, cyanide-resistant respiration, alternative oxidase. Lipid metabolism. Nutrient assimilation: nitrogen and sulphur cycle, nitrate and ammonium assimilation, biological nitrogen fixation, sulphur and phosphate assimilation, nutritional deficiencies.
The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it
( reference books)
Plant Physiology (in English language); Taiz and Zeiger; Sinuaver associates (fifth edition)
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6
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BIO/04
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48
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Basic compulsory activities
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3
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BIO/04
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16
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10
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Core compulsory activities
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ITA |