Optional group:
OPZIONALI - (show)
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20401076 -
MICROBIAL BIOTECHNOLOGY
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20401081 -
ETHOLOGY AND ANIMAL BEHAVIOUR
(objectives)
PROVIDING STUDENTS WITH CONCEPTUAL BASIS FOR: (a) UNDERSTANDING BIOLOGICAL BASIS OF BEHAVIOR (b) UNDERSTANDING BEHAVIOR AS AN EMERGENT PROPERTY OF THE SYSTEM “ORGANISM” (c) AN EVOLUTIONARY APPROACH TO THE STUDY OF BEHAVIOR. PROMPTING STUDENTS’ CRITICAL THINKING BY MEANS OF APPROPRIATE READINGS AND DISCUSSIONS. THEREFORE STUDENTS WILL BE GIVEN TOOLS TO CORRECTLY EVALUATE A SCIENTIFIC HYPOTHESIS, CHOOSE APPROPRIATE TOOLS OF INVESTIGATION AND INTERPRET RESULTS.
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CAROSI MONICA
( syllabus)
Cultural skills (Knowledge of:) The course offers the conceptual bases for (A) understanding the biological bases of behaviour (B) understanding behaviour as an emergent property of the organism (C) an evolutionary approach to the study of behaviour .
Methodological skills (Knowing how to perform:) acquire the critical thinking through readings and discussions, in order to correctly evaluate a research hypothesis, choose research tools and interpret results.
SYLLABUS
Ethology: historical outline. Classic and operant conditioning. The three Nobel awards, founders of modern ethology: K. Lorenz, K. Von Frisch, and N. Tinbergen. Sociobiology and behavioral ecology. The evolutionary approach to the study of behaviour. Levels of analysis in the study of behaviour: questions on “how” and “why”. The study of causation and ontogenesis (proximate causes), survival value and evolution (ultimate causes).
(1) Behavioural control. Exogenous factors and endogenous factors. Sensory filtering. Stimuli and signals. Fixed action patterns, evocative stimuli, innate triggering mechanism. Biorhythms. The biological clock. The hormonal state. The behavioural decision: the motivation. Spatial orientation. Compasses. The olfactory map.
(2) Ontogenesis of behaviour. Role of genes and the environment. Embryology and behaviour: pre-natal ontogeny. Embryonic development of motility, sensitivity and sensory-motor sequences. Neurogenesis. Embryo learning. Postnatal ontogenesis. Behavioural endocrinology. The interactive theory of development, the organisational-activational hypothesis: development organisation, sexualisation of the brain, neural activation. Pre and post-natal learning. Imprinting and hormonal control of imprinting. Non-associative, associative, and complex learning. Behavioural control: nervous system and hormonal system.
(3) The adaptive value of behaviour. The comparative approach to the study of behaviour. Signal evolution and communication. Communication: definitions, honest communication and deception. Ritualisation. Natural and artificial selection. Hybridisation between lines. Behavioural characters and strategies. Optimal strategies and evolutionarily stable strategies. Predatory and anti-predatory strategies: the arms race. Mimicry. Reproductive and social behaviour. Optimal strategies, fitness and reproductive success. Sexuality and innovation. Sexual selection: partner choice and competition. Sexual selection and sexual dimorphism. Conflict strategies between sexes. Differential reproductive success of females and males. Parental investment. Mating systems and social life. Sociality and group life: costs and benefits of socializing; the kin selection; altruism and evolution. Game theory.
( reference books)
RECOMMENDED TEXTBOOKS:
- Alcock, J. 2007. Etologia, un approccio evolutivo. Zanichelli
- Manning, A. e Stamp Dawkins, M. 2003. Il comportamento animale. Bollati Boringhieri.
- Krebs J.R. e Davies N.B. 2002. Ecologia e comportamento animale. Bollati Boringhieri
TEXTBOOKS FOR FURTHER READINGS:
- Bolhuis, J.J. & Giraldeau, l. 2005. The behavior of animals: mechanisms, function and evolution.
- Campan, R. & Scapini, F. 2004. Etologia. Zanichelli
- Nelson, N.J. 2002. An introduction to behavioral endocrinology. Sinauer Associates, Sunderland
- Andersson, M 1994. Sexual selection. Princeton University Press
- Mainardi, D. 1992. Dizionario di etologia. Einaudi
- Martin, P. & Bateson, P. 2003. La misurazione del comportamento. Una guida introduttiva. Liguori editore
All textbooks can be found in the library
Students are provided with a document that lists for each lesson topic which textbook and which chapters are most relevant for the study, and for each topic it lists recent scientific papers for further study. Students are also provided with PDFs of lessons and of scientific papers suggested.
The professor receives every day from 9.00 to 10.00 by appointment via email: monica.carosi@uniroma3.it
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20401221 -
MOLECULAR GENETIC AND CYTOGENETIC TECHNIQUES
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20401221-2 -
MODULO 2
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3
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BIO/18
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30
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20401221-1 -
MODULO 1
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20401283 -
HUMAN GENETICS
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20401283-2 -
MODULO 2
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1
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BIO/18
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MODULO 1
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20401653 -
BIOCHEMISTRY AND MOLECULAR CLINICAL BIOLOGY
(objectives)
Main goals of the course are: i) to provide an overview of the activities of a clinical laboratory looking at the organizational context and current business; ii) to illustrate the various stages which are at the basis of main analytical methods, either molecular or biochemical, used in biomedical laboratories for modern diagnostic purposes; iii) to provide the key of Interpretation for the obtained results from a pathophysiological point of view. To promote a critical approach towards laboratori diagnosis.
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20401656 -
BIOGEOGRAPHY
(objectives)
AFTER THE COURSE, THE STUDENTS SHOULD BE ABLE TO: 1) DISCUSS THE HISTORICAL AND ECOLOGICAL FACTORS THAT AFFECTED THE GEOGRAPHICAL DISTRIBUTION OF ANIMALS AND PLANTS; 2) FORMULATE HYPOTHESES AND MODELS EXPLAINING THE SHAPE, SIZE AND POSITION OF THE GEOGRAPHIC RANGE OF A TAXON; 3) DISCUSS EVENTS OF RANGE EXPANSION OR CONTRACTION, DUE TO CLIMATIC CHANGES OR HABITAT TRANSFORMATION CAUSED BY HUMAN ACTIVITIES; 4) DESCRIBE THE FAUNA, FLORA AND VEGETATION OF EACH CONTINENT, IN RELATION TO PAST AND PRESENT GEOGRAPHICAL AND ECOLOGICAL FACTORS; 5) BUILD PREDICTIVE MODELS FOR BIODIVERSITY CONSERVATION AND MANAGEMENT, IN CONNECTION WITH CLIMATE CHANGES AND LANDSCAPE ALTERATION.
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6
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BIO/05
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20401660 -
ANALYTICAL CHEMISTRY
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20401665 -
MANAGEMENT AND PLANNING OF HISTORICAL GARDENS AND ARCHAEOLOGICAL SITES
(objectives)
STUDY THE RELATIONSHIP BETWEEN MAN & NATURE IN THE ANCIENT TIME. STUDY BIODETERIORATION RELATED TO THE VEGETATION IN THE ARCHAEOLOGICAL AREAS. ANALYSE HOW WAS THE PLANNING OF ARCHAEOLOGICAL PARKS IN ITALY AND DESCRIBE THE PROBLEMS OF MANAGEMENT. STUDY HISTORICAL PARKS, GIVING EXAMPLES FROM THE ASSYRO-BABYLONIANS, EGYPTIANS, GREEK-ROMANS, MEOIOEVAL TIMES TO MODERN AND CONTEMPORARY AGE. DESCRIBE THE PROBLEMS OF MANAGEMENTS OF HISTORICAL GARDENS. GIVE PRACTICAL FIELD EXAMPLES, VISITING AREAS SUCH AS COLOSSEUM AND HORTI FARNESIANI ON THE PALATINE, ARCHAEOLOGICAL AREAS IN THE APPIA ANTICA, AND OTHER HISTORICAL PARKS IN THE CENTRAL ITALY.
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20401668 -
ENVIRONMENTAL MICROBIOLOGY
(objectives)
THE MAIN AIMS OF THE COURSE ARE:
1) RECOGNIZE MAJOR GROUPS OF MICROORGANISMS AND UNDERSTAND FACTORS INFLUENCING THEIR ENVIRONMENTAL DISTRIBUTION AND INTERACTIONS WITH HIGHER ORGANISMS;
2) LEARN ABOUT ROLES PLAYED BY MICROBES IN SOIL FORMATION, MINERAL WEATHERING, CARBON AND NITROGEN CYCLING;
3) LEARN ABOUT CULTURE-BASED AND CULTURE-INDEPENDENT MICROBIOLOGICAL METHODS;
4) GAIN LITERACY IN CRITICAL READING AND ANALYSIS OF SCIENTIFIC LITERATURE IN THE FIELD OF ENVIRONMENTAL MICROBIOLOGY.
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20401668-2 -
MODULO 2
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DALMASTRI Claudia
( syllabus)
1. Evolution and systematic of microorganismsOrigin of bacteria; molecular phylogenesis; 16S rRNA gene and evolution; basis of microbial systematic 2. Metabolic diversity of microbesChemolitotrophy; fermentation; anaerobiosis; phototrophy3. The principal phylogenesic groups of Bacteria and ArcheaProteobacteria, Firmicutes, Bacteroidetes, Ipertermofili4. Functional diversity of microbes5. Methods in microbial ecologyCulture-based methods Cultivation-independent techniques: microscope; genetic analysis; genome and metagenomics; functional analysis6. Microbial ecosystems Principles of ecology Microbe environment: microbial interactions (Quorum sensing; Biofilm) Terrestrial environment: soil Aquatic environment: lakes, oceansExtreme environments (deep sea; hydrothermal springs)7. Microbial role in nutrient cycles Carbon, nitrogen, sulfur, others8. Symbiosis between microorganisms and between microorganisms and different organisms Plants; rhizosphere; root nodules Mammals; microbiome: bowel and rumen9. Microorganisms in anthropized environments Bioremediation of contaminated sites; water treatment; biocorrosion; mineral recovery from mines
( reference books)
Brock. Biologia dei microrganismi. Microbiologia generale, ambientale e industriale. Madigan et al., Pearson Editore (XIV ed.).
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20401668-1 -
MODULO 1
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DALMASTRI Claudia
( syllabus)
1. Evolution and systematic of microorganismsOrigin of bacteria; molecular phylogenesis; 16S rRNA gene and evolution; basis of microbial systematic 2. Metabolic diversity of microbesChemolitotrophy; fermentation; anaerobiosis; phototrophy3. The principal phylogenesic groups of Bacteria and ArcheaProteobacteria, Firmicutes, Bacteroidetes, Ipertermofili4. Functional diversity of microbes5. Methods in microbial ecologyCulture-based methods Cultivation-independent techniques: microscope; genetic analysis; genome and metagenomics; functional analysis6. Microbial ecosystems Principles of ecology Microbe environment: microbial interactions (Quorum sensing; Biofilm) Terrestrial environment: soil Aquatic environment: lakes, oceansExtreme environments (deep sea; hydrothermal springs)7. Microbial role in nutrient cycles Carbon, nitrogen, sulfur, others8. Symbiosis between microorganisms and between microorganisms and different organisms Plants; rhizosphere; root nodules Mammals; microbiome: bowel and rumen9. Microorganisms in anthropized environments Bioremediation of contaminated sites; water treatment; biocorrosion; mineral recovery from mines
( reference books)
Brock. Biologia dei microrganismi. Microbiologia generale, ambientale e industriale. Madigan et al., Pearson Editore (XIV ed.).
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20401669 -
EXPERIMENTAL MODELS IN BIOLOGY
(objectives)
THE COURSE PROVIDES BASICS CONCERNING THE EXPERIMENTAL MODELS MOST COMMONLY USED IN BIOLOGICAL RESEARCH.
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20401671 -
GENERAL PATHOLOGY
(objectives)
Aims of the course:
• To acquire the basic knowledge on the human disease causes and their pathogenic mechanisms.
• To interpret the pathogenetic and physiopathogenic mechanisms of the human diseases.
• To know the operations turned to the analysis of the pathogenetic and physiopathogenic mechanisms of the human diseases and to interpret their results.
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20401674 -
CARTOGRAPHY AND G.I.S. APPLIED TO ECOLOGY
(objectives)
TO PROVIDE THE THEORETICAL CONCEPTS AND PRACTICAL TOOLS IN GEOGRAPHY, CARTOGRAPHY, AND STATISTICAL ANALYSIS FOR ECOLOGICAL ADVANCES, THROUGH THE USE OF CASE STUDIES AND PRACTICAL APPLICATIONS.
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20401777 -
PUBLIC HEALTH
(objectives)
General objectives
At the end of the course the student will be able to design epidemiological studies to address public health problems and to identify activities to prevent chronic and infectious diseases.
Specific objectives
- to know the major mortality and morbidity indicators
- to identify the more appropriate epidemiological study to describe the burden of major diseases in the population and to assess the relation between diseases and possible risk factors associated to them
- to interpret epidemiological data
- to discuss the importance of the health education and health promotion
- to list and discuss problems related to water supply, sanitation and waste management
- to argue about necessary controls/monitoring to protect the environment and the community
- to design health promotion programmes
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MED/42
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20401782 -
MOLECULAR BIOTECHNOLOGY
(objectives)
THE AIM OF THE COURSE IS TO PROVIDE METHODOLOGIES AND TECHNOLOGIES KNOWLEDGE OF BASIC AND APPLIED ADVANCED RESEARCH IN MOLECULAR BIOTECHNOLOGIES.
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20401782-2 -
MODULO 2
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Derived from
20401782 BIOTECNOLOGIE MOLECOLARI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.
( reference books)
Recommended textbooks:1. ANALISI DEI GENI E GENOMI, REECE, EDISES.2. DNA RICOMBINANTE, J.D.WATSON, A.A. CAUDY, R.M. MYERS E J.A. WITKOWSKI, ZANICHELLI.Lecture slides will be providedThe professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it
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BIO/11
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20401782-1 -
MODULO 1
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Derived from
20401782 BIOTECNOLOGIE MOLECOLARI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.
( reference books)
Recommended textbooks:1. ANALISI DEI GENI E GENOMI, REECE, EDISES.2. DNA RICOMBINANTE, J.D.WATSON, A.A. CAUDY, R.M. MYERS E J.A. WITKOWSKI, ZANICHELLI.Lecture slides will be providedThe professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it
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5
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BIO/11
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20401786 -
GENERAL ENTOMOLOGY
(objectives)
THE MAIN FORMATIVE TARGETS ARE: 1) THE ACQUISITION OF A BASIC KNOWLEDGE ON MORPHOLOGY, ANATOMY, PHYSIOLOGY AND EVOLUTION OF THE MAIN INSECT GROUPS. 2) THE ACQUISITION OF BOTH THEORETICAL AND PRACTICAL KNOWLEDGE ON SYSTEMATICS AND CLASSIFICATION OF THE HEXAPODA. 3) THE CONSTRUCTION OF A SOLID CULTURAL BACKGROUND FOR AN ADVANCED DISCUSSION ON CAUSES AND EFFECTS OF THE AMAZING EVOLUTIONARY SUCCESS AND DIVERSITY OF INSECTS AND ON THEIR IMPORTANCE IN BASIC AND APPLIED STUDIES. 4) THE ACQUISITION OF THE TOOLS FOR THE INSECT TAXONOMIC IDENTIFICATION, EXPECIALLY FOR THOSE ORDERS AND FAMILIES PRESENT IN THE ITALIAN FAUNA. 5) TO STIMULATE THE CURIOSITY AND THE ABILITY OF CRITICALLY OBSERVE THE NATURE. 6) THE SELF-EVALUATION BY THE STUDENT OF THE COMPETENCE WHITIN THE ANIMAL BIOLOGY, AND IN PARTICULAR IN THE ENTOMOLOGY.
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20401786-2 -
MODULO 2
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DI GIULIO ANDREA
( syllabus)
PART 2) INSECT PHYLOGENY. CLASSIFICATION: DIAGNOSTIC CHARACTERS AT ORDER AND MAIN FAMILY LEVEL (ESPECIALLY TO THOSE PRESENT IN ITALY). METHODS OF STUDY, COLLECTION, PREPARATION AND PRESERVATION.THE SECOND PART OF THE COURSE INCLUDES, DURING THE LESSONS PERIOD, FOUR LABORATORY PRACTICES (EACH OF CA. TWO HOURS) AND TWO DAILY FIELD PRACTICE (ONE IN THE MIDDLE AND ONE AT THE END OF THE COURSE). LAB PRACTICAL ACTIVITIES CONCERN THE EXAMINATION OF SLIDES AND PRESERVED SPECIMENS BELONGING TO THE MAIN ORDERS AND FAMILIES; SOME ANATOMICAL DISSECTIONS WILL BE ALSO PERFORMED. THE FIELD EXPERIENCE IS AIMED TO SHOW SPECIES DIVERSITY AND ECOLOGICAL ASSEMBLAGES OF BOTH TERRESTRIAL AND WATER INSECTS, SHOWING THE MOST COMMON METHODS OF SAMPLING.
( reference books)
P.J. GULLAN & P.S. CRANSTON “LINEAMENTI DI ENTOMOLOGIA” ZANICHELLI orR.G. DAVIES “LINEAMENTI DI ENTOMOLOGIA” ZANICHELLI OTHER TEXTBOOKS (THE CHOICE HAS TO BE DISCUSSED WITH THE PROFESSOR):G. GRANDI “ISTITUZIONI DI ENTOMOLOGIA GENERALE” EDIZIONI CALDERINIV.B. WIGGLESWORTH “LA VITA DEGLI INSETTI” GARZANTI R.F. CHAPMAN “THE INSECTS: STRUCTURE AND FUNCTION” HODDER & STOUGHTON, LONDON C. GILLOT “ENTOMOLOGY” PLENUM PRESS, NEW YORK O.W. RICHARDS & R.G. DAVIES “IMM’S GENERAL TEXTBOOK OF ENTOMOLOGY” CHAPMAN & HALL, LONDON VOL. I AND IIGENERAL INSECT GUIDES (STUDENT CHOICE) ARE RECOMMENDED FOR BOTH LAB AND FIELD ACTIVITIES AS WELL AS FOR THE STUDY OF THE SYSTEMATIC PART OF THE COURSE.Office Hours: every day of the week by appointment by email (andrea.digiulio@uniroma3.it)
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20401786-1 -
MODULO 1
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DI GIULIO ANDREA
( syllabus)
PART 1) BASIC AND APPLIED ENTOMOLOGY. ARTHROPOD PHYLOGENY. ORIGIN, CAUSES AND SIGNIFICANCE OF THE INSECT SUCCESS AND DIVERSITY. INTEGUMENT; STRUCTURE OF THE INTEGUMENT AND CUTICLE; COLORS. BODY REGIONS; SCLERITES; SEGMENTS, APPENDICES AND WINGS. MUSCULATURE AND ENDOSCHELETON; ADAPTATION TO THE MOVEMENT IN AERIAL AND ACQUATIC ENVIROMENTS. RESPIRATORY APPARATUS AND RESPIRATION; RESPIRATION IN WATER. EXCRETION. CEPHALIC APPENDAGES AND MOUTHPARTS; ANATOMY AND PHYSIOLOGY OF THE DIGESTIVE SYSTEM; FEEDING STRATEGIES. CIRCULATORY SYSTEM AND BLOOD. REPRODUCTION: STRUCTURE AND FUNCTIONAL MORPHOLOGY OF SEGMENTS AND GENITAL TRAITS; REPRODUCTIVE BEHAVIORS; EGGS; SPERMS. EMBRYONIC AND POSTEMBRYONIC DEVELOPMENT. ENDOCRINE SYSTEM: HORMONS AND ENDOCRIN ORGANS. HEXOCRIN GLANDS. NERVOUS SYSTEM AND SENSE ORGANS. SIMPLE AND COMPOUND EYES. PRODUCTION OF LIGHT AND SOUNDS. CRIPTISM AND MIMICRY. PHEROMONS AND COURTSHIP; PARENTAL CARES; INSECT SOCIETIES. PREDATION; PARASITISM; SYMBIOSIS. ORIENTATION; MIGRATIONS; DISPERSION. INSECT BIOGEOGRAPHY. INSECT CONSERVATION.
( reference books)
P.J. Gullan & P.S. Cranston “Lineamenti di Entomologia” Zanichelli R.G. Davies “Lineamenti di Entomologia” Zanichelli G. Grimaldi “Evolution of the Insects”R.F. Chapman “The Insects: structure and function” Hodder & Stoughton, London C. Gillot “Entomology” Plenum Press, New York O.W. Richards & R.G. Davies “Imm’s general textbook of entomology” Chapman & Hall, London Vol. I e II M. Chinery “Guida agli insetti d’Europa” Muzzio Office Hours: every day of the week by appointment by email (andrea.digiulio@uniroma3.it)
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20401787 -
ETHNOBOTANY AND ETHNOZOOLOGY
(objectives)
ANALYSIS OF ECOLOGICAL AND CULTURAL RELATIONS BETWEEN HUMANS AND LIVING BEINGS, THROUGH AN HISTORICAL AND GEOGRAPHIC SURVEY AT DIFFERENT LEVELS OF CIVILIZATION. PERSPECTIVES OF THE RECOVERY OF POPULAR TRADITIONS.
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20401788 -
STRATEGIC ENVIRONMENTAL ASSESSMENT
(objectives)
To provide the basics in terms of technical, legal, scientific and cultural issues on environmental assessment tools, with particular reference to the Environmental Impact Assessment, the Strategic Environmental Assessment, the assessment of impacts, the indicators for the monitoring and reporting of environmental . The knowledge acquired by the students, integrated with the content of other courses within the degree course (Technical Cartographic and GIS, Statistics, Applied Ecology, Management of Ecosystems, Biodiversity, etc..) contributes to develop professional figures in environmental monitoring.
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20401821 -
IMMUNOLOGY
(objectives)
to deeply understand cellular and molecular mechanisms of immune response and their role in health and disease.
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20401875 -
METHODS AND TECHNIQUES OF EDUCATION AND PROMOTION OF NATURALISTIC AND ENVIRONMENTAL ISSUES
(objectives)
Students’ grow in knowledge, skills and abilities in order to become a professional in the fields of education and naturalistic and environmental dissemination.
Making students aware of the professions related to dissemination and education in the field of nature and environment as potential job opportunities for specialists of management and protection of ecosystems.
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20401990 -
CELLULAR BIOCHEMISTRY
(objectives)
The aim of the course is to introduce students to the “inorganic biofactors”, starting from their biochemical role to the analysis of their impact on human health. In the second part of the course we will focus on the “omics”, which represents a broad discipline of science (from trascriptomics to metabolomics) intended for analyzing system biology and for characterizing the biochemical events associated to transcripts, proteins and metabolites variations in fluids, organs and tissues.
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Derived from
20401990 BIOCHIMICA CELLULARE in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 N0 DI MASI ALESSANDRA, ASCENZI PAOLO
( syllabus)
HEME-PROTEINS. HEME METABOLISM AND RELATED DISEASES. HEME BIOSYNTHESIS AND RELATED DISEASES. IRON METABOLISM AND RELATED DISEASES. INORGANIC BIOFACTORS: OXYGEN, CARBON MONOXIDE, NITROGEN MONOXIDE, HYDROGEN SULFIDE. BIOCHEMICAL AND CELLULAR ROLES OF THE INORGANIC BIOFACTORS. ROLE IOF THE INORGANIC BIOFACTORS IN HUMANN DISEASES ONSET. THE THERAPEUTIC USE OF THE INORGANIC BIOFACTORS. REACTIVE OXYGEN AND NITROGEN SPECIES. MITOCHONDRIAL DISEASES. THE ENERGETIC MATTER OF CANCER CELLS. THE METABOLIC REQUIREMENTS OF CANCER CELLS: THE WARBURG EFFECT.
( reference books)
SCIENTIFIC PAPERS SUPPLIED BY THE TEACHER.
Support texts:
Siliprandi & Tettamanti "Biochimica Medica - strutturale, metabolica e funzionale" Ed. Piccin
- Chiurchiù & Maccarrone "I radicali liberi e la loro rilevanza in biomedicina: dai meccanismi molecolari alle prospettive terapeutiche" Ed Piccin
RECEPTION TIME: Monday 10 am
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20401992 -
MICROBIAL BIOTECHNOLOGY LABORATORY
(objectives)
THE MAIN AIMS OF THE COURSE ARE:
A) ACQUISITION OF APPROPRIATE METODOLOGIES AND LANGUAGES FOR THE ISOLATION, OBSERVATION AND DESCRIPTION OF INDUSTRIAL MICROORGANISMS
B) ACQUISITION OF THE BASIC KNOWLEDGE FOR THE DRAFTING AND EXECUTION OF AN EXPERIMENTAL PROTOCOL
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20401992-2 -
MODULO 2
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Derived from
20401992 LABORATORIO DI BIOTECNOLOGIE DEI MICRORGANISMI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
The course aims at providing to the student:- adequate knowledge and understanding on advanced methodologies in microbial biotechnology, appropriate methodologies and communication skills for the isolation, observation, description and engineering of industrial microorganisms;- adequate applied knowledge for drafting and executing experimental protocols, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).ProgrammeDuring the laboratory practices and the corresponding theoretic lessons, the following topics will be covered:- isolation of microorganisms of industrial relevance from the environment, preparation of growth media and cultivation techniques;- transposon–based mutagenesis (generation and screening of mutant libraries);- genetic transfer processes (conjugation and transformation);- use of whole-cell bacterial biosensors for gauging specific molecules produced by other microorganisms.The following topics will be covered in additional theoretic lessons: - development of innovative therapeutic strategies based on biotechnological processes;- development of biosensors and reporter systems for the identification of new drugs;- application of synthetic biology to microbial biotechnology;- properties of the regulatory networks and their application to biotechnological processes.
( reference books)
Selected topics will be available on suggested Microbiology and Microbial Biotechnology books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices. Innovative teaching will be promoted by cloud-sharing of power-point presentations illustrating the main topics of the course. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.Students will be received every Monday, from 3:00 to 4:00 pm. Students can be received at the best of their convenience if an appointment has been fixed at the end of the lessons or by email.
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20401992-1 -
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Derived from
20401992 LABORATORIO DI BIOTECNOLOGIE DEI MICRORGANISMI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
Programma (ENG)The course aims at providing to the student:- adequate knowledge and understanding on advanced methodologies in microbial biotechnology, appropriate methodologies and communication skills for the isolation, observation, description and engineering of industrial microorganisms;- adequate applied knowledge for drafting and executing experimental protocols, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).ProgrammeDuring the laboratory practices and the corresponding theoretic lessons, the following topics will be covered:- isolation of microorganisms of industrial relevance from the environment, preparation of growth media and cultivation techniques;- transposon–based mutagenesis (generation and screening of mutant libraries);- genetic transfer processes (conjugation and transformation);- use of whole-cell bacterial biosensors for gauging specific molecules produced by other microorganisms.The following topics will be covered in additional theoretic lessons: - development of innovative therapeutic strategies based on biotechnological processes;- development of biosensors and reporter systems for the identification of new drugs;- application of synthetic biology to microbial biotechnology;- properties of the regulatory networks and their application to biotechnological processes.
( reference books)
Selected topics will be available on suggested Microbiology and Microbial Biotechnology books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices. Innovative teaching will be promoted by cloud-sharing of power-point presentations illustrating the main topics of the course. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics. Students can be received at the best of their convenience if an appointment has been fixed at the end of the lessons or by email.
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20402204 -
BIO-ORGANIC CHEMISTRY
(objectives)
The course will address the chemical transformations shared by organic chemistry and biological systems. Indeed, although the functional group elaborations are formally the same, the followed reaction pathways result to be quite different with respect to the experimental conditions of both organic syntheses and biological processes. In such a context, the enzymatic action modes in living systems will be compared with the key role performed by temperature, pressure, pH, catalysts and solvents. Keeping this in mind, a few of the most well-known chemical reaction mechanisms will be studied, underlining, step by step, the main similarities and distinctions with the analogous biological transformations
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22402256 -
EVOLUTIONARY PARASITOLOGY
(objectives)
Offering the students a new perspective in the study of parasitology, by means of an ecological- evolutionary and comparative approach. The intimate and intricate nature of the association between host and parasite, has traditionally been studied by giving emphasis on its pathogeniticy for non human animal and human hosts. In contrast, this course offers the student the opportunity to deepen topics such as adaptive strategies of parasitic life-style, the impact of parasitism on the community structure or else the role of a parasite in the trophic energy transfer in the community. Emphasis will be given to the host as key element in the parasite environment and, as such, its important role as a selective pressure. The student will be provided with a dynamic course made of lectures, workshops and tutorials on taxa parasites
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22402256-1 -
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BIO/05
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22402256-2 -
MODULO 2
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1
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BIO/05
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10
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22402257 -
GENERAL AND APPLIED ANIMAL VIROLOGY
(objectives)
OBJECTIVS: TO ACQUIRE A SOLID FOUNDATION IN ANIMAL VIROLOGY AND THEIR APPLICATION.
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22402257-1 -
MODULO 1
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Derived from
22402257 VIROLOGIA ANIMALE GENERALE ED APPLICATA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
Module 1:The course describes and compares the replication cycles of different animal viruses that infect vertebrate hosts to gain insight into: origin of viruses, structure, evolution, virus-cell interactions, host defense mechanisms and strategies of viral immunoevasion, methods of detection, identification and titration, antiviral drugs and profilaxis of viral infections. In particular, it will be described viruses of the following viral families: picornaviridae, ortomixoviridae, paramixoviridae, rabdoviridae, retroviridae, hepadnaviridae, flaviviridae, parvoviridae, poliomaviridae, papillomaviridae, adenoviridae, herpesviridae, poxviridae. Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
WHERE TO STUDY:Adopted text book:- Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press+- Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (to study adenoviral, adeno-associated, retroviral and lentiviral vectors + oncolitic adenoviruses)- Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159.It is available a CD with supplementary materials at the box office of “segreteria didattica biologia“
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BIO/19
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40
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22402257-2 -
MODULO 2
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Derived from
22402257 VIROLOGIA ANIMALE GENERALE ED APPLICATA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
Module 2. It will be introduced applied virology and specific examples will be provided (i.e., vaccine development, viral vectors and oncolytic viruses). Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
WHERE TO STUDY:Adopted text book:- Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press+- Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (to study adenoviral, adeno-associated, retroviral and lentiviral vectors + oncolitic adenoviruses)- Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159.It is available a CD with supplementary materials at the box office of “segreteria didattica biologia“
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10
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20402024 -
BIOCHEMICAL PHYSICS
(objectives)
THE COURSE AIMS TO PROVIDE THE STUDENT WITH BASIC KNOWLEDGE ON THERMODYNAMICS AND KINETICS OF BIOLOGICAL PROCESSES, PARTICULARLY IN RELATIONSHIP WITH THE EXPERIMENTAL ACTIVITY IN THE LABORATORY.
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Derived from
20402024 BIOCHIMICA FISICA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 N0 ANTONINI GIOVANNI
( syllabus)
Cultural competences (Knowledge of :) The Course aims to provide students with basic knowledge relating to thermodynamics and the kinetics of biological processes, particularly in relation to experimental activities in the laboratory.
Methodological skills (Know How to:) acquire and use experimental data relating to: to the study of interactions between proteins and ligands, enzyme kinetics and kinetic of binding
Program:
1) Study of the interaction between receptors and ligands: methods at equilibrium and transient kinetics:
a) At equilibrium (thermodynamic parameters: Kd: dissociation constant at equilibrium, cooperativity, number of binding sites)
b) Rapid kinetics (kinetic parameters: k: velocity constants)
2) Study of enzymatic activity: steady state kinetics
a) In Steady State (when [ES] is almost constant during experimental observation)
b) In the presence of inhibitors
3) Time-resolved optical spectroscopy and complex kinetics
( reference books)
Recommended textbooks:
M. C. Bonaccorsi di Patti et al. Metodologie Biochimiche - Casa Editrice Ambrosiana
Ninfa/Ballou Metodologie di base per la Biochimica e la Biotecnologia - ed. Zanichelli
Reed et al. Metodologie di Base per le Scienze Biomolecolari- ed. Zanichelli
P. Silvestroni, Fondamenti di chimica - Casa Editrice Ambrosiana
D.M. Etter Introduction to MATLAB 7– Pearson Education, Inc.
Software to use
EXCEL (Office) or CALC (Open Office) * (for linear methods)
MATLAB (Mathworks) or OCTAVE (GNU Octave) * (for spectral analysis and non-linear methods)
GEPASI (P. Mendes et al.) * Or COPASI (Copasi dev. Group) * (for complex reactions)
(*) indicates free software
Lecture notes and calculation exercises will be provided
The professor receives every day from
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20402025 -
Bioindication and Environmental Monitoring
(objectives)
Acquisition of specific knowledge on bioindication and its applications, particularly with regard to plant and animal organisms used as biological indicators of the environmental status. Knowledge of the experimental methods and data processing for the environmental monitoring.
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20402025-1 -
MODULO 1
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BIO/02
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20402025-2 -
MODULO 2
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BIO/02
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20402506 -
MOLECULAR BASIS OF PLANT-ENVIRONMENT INTERACTION
(objectives)
TO INCREASE KNOWLEDGE CONCERNING FUNDAMENTAL PROCESSES UNDERLYING PLANT ACCLIMATION TO DIFFERENT ENVIRONMENTAL STRESSES, USEFUL IN DEVELOPING NEW STRATEGIES FOR IMPROVING CROP PRODUCTIVITY UNDER CHANGING ENVIRONMENTAL CONDITIONS IN THE CONTEXT OF SUSTAINABLE AGRICULTURE. BASIC KNOWLEDGE WILL BE PROVIDED ON PLANT EMPLOYMENT IN PHARMACOLOGICAL, NUTRACEUTICAL AND ENVIRONMENTAL FIELD.
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20402507 -
Diversity and Ecology of the Soil Fauna
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20402508 -
NATURAL PRODUCTS AND ENVIRONMENTAL CHEMISTRY
(objectives)
THE COURSE AIMS AT GIVING:
1) A GENERAL KNOWLEDGE OF THE BIOSYNTHETIC PATHWAYS OF SECONDARY METABOLISM;
2) INFORMATION ABOUT THE MAIN NATURAL PRODUCTS DERIVED FROM MICROORGANISM, PLANTS AND ANIMALS AND THEIR ROLE IN NATURAL ENVIRONMENT;
3) KNOWLEDGE ON THE STRUCTURE/BIOLOGICAL ACTIVITY RELATIONSHIP OF THE MAIN SECONDARY METABOLITES USED IN PHARMACEUTICAL AND HEALTHCARE INDUSTRY.
4) ATTAINMENT OF THE BASIC EDUCATIONAL BACKGROUND NECESSARY TO UNDERSTAND THE CHEMICAL EQUILIBRIA THAT REGULATE THE ORIGINS, THE TRANSPORT, THE TRANSFORMATIONS AND THE EFFECTS OF THE ENVIRONMENTAL CHEMICAL SPECIES, EITHER OF NATURAL OR HUMAN ORIGIN.
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TOFANI DANIELA
( syllabus)
INTRODUCTION TO NATURAL PRODUCTS CHEMISTRY. MAIN FEATURES OF SECONDARY METABOLISM.
ACETATE PATHWAY. PROSTAGLANDINS, AROMATIC POLYKETIDES, ANTHRAQUINONES, CANNABINOIDS, AFLATOXINS.
SHIKIMATE PATHWAY. SHIKIMIC ACID BIOSYNTHESIS. CINNAMIC ACIDS. LIGNINES. PHENYLPROPANOIDS. BENZOIC ACIDS. COUMARINS. FLAVONOIDS. TANNINS. ISOFLAVONES. TERPENOID QUINONES.
MEVALONATE PATHWAY. MEVALONIC ACID BIOGENESIS. BIOSYNTHESIS OF DIFFERENT CLASSES OF TERPENES. IRIDOIDS. GIBBERELLINS. HORMONES. ANIMAL AND HUMAN STEROIDS. PHYTOSTEROLS.
ALKALOIDS: DEFINITION AND NOMENCLATURE. BIOSYNTHESIS AND BIOLOGICAL ACTIVITY OF THE MAIN CLASSES OF NATURAL ALKALOIDS: COCAINE, NICOTINE, CURARE, MORPHINE, LOGANIN, LYSERGIC ACID, CONIINE.
CARBOHYDRATES: GENERALITIES. CYANOGENIC GLYCOSIDES. STREPTOMYCINS.
PEPTIDIC SECONDARY METABOLITES. INTERFERONS. OPIOID PEPTIDES. PEPTIDE TOXINS: RICIN AND BOTULIN. PENICILLINS.
INTRODUCTION TO ENVIRONMENTAL CHEMISTRY. THE SPHERES IN WHICH THE EARTH IS DIVIDED.
THE ATMOSPHERE: OZONE LAYER FORMATION AND DEPLETION; CFCS AND HALONS. OXIDANT AND POLLUTANTS SPECIES. THE GREENHOUSE EFFECT. THE KYOTO’S PROTOCOL.
THE HYDROSPHERE: PHYSICAL AND CHEMICAL PROPERTIES OF WATER AND WATER BODIES. TIC, TOC, TC. EASY TO OXIDIZE ORGANIC POLLUTANTS (COD, BOD5) AND PERSISTENT ORGANIC POLLUTANTS (POP). SHORT AND LONG TERM TOXICITY (LD50 AND LOD50). NOEL AND ADI.
BIOACCUMULATION AND BIO-MAGNIFICATION. METABOLISM OF A FEW PESTICIDES.
( reference books)
P.M. DEWICK : MEDICINAL NATURAL PRODUCTS. A BIOSYNTHETIC APPROACH. THIRD EDITION. WILEY..
C. BAIRD, M. CANN “ENVIRONMENTAL CHEMISTRY”, FIFTH EDITION.
SLIDES OF THE LESSONS AND ADDITIONAL MATERIAL (PW PROTECTED) ARE AVAILABLE ON LINE AT THE ADDRESS: HTTP://HOST.UNIROMA3.IT/CENTRI/CISDIC/INDEX.HTM
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20402509 -
ECOLOGY AND MANAGEMENT OF COASTAL ECOSYSTEMS
(objectives)
MAJOR GOALS OF THIS COURSE ARE: TO ACQUIRE AN APPROPRIATE KNOWLEDGE OF THE BIODIVERSITY AND OF GENERAL PRINCIPLES OF THE ECOLOGY OF COASTAL ECOSYSTEMS AND THEIR RELATIONSHIP WITH OTHER ECOLOGICAL BRANCHES. OTHER GOAL OF THIS COURSE IS THE GROWTH OF A THOROUGH INTEREST TO ECOLOGICAL PROBLEMS DISCUSSING THE RELATIONSHIP BETWEEN MAN AND NATURE FOCUSING IN THE COASTAL ENVIRONMENT. THE COURSE IS ALSO INTENDED TO DEVELOP BASIC INSIGHTS FOR THE ACQUISITION, ORGANIZATION AND INTERPRETATION OF ECOLOGICAL DATA RECORDED IN COASTAL ECOSYSTEMS.
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20402509-1 -
MODULO 1
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ACOSTA ALICIA TERESA ROSARIO
( syllabus)
Coastal ecosystems, the transition between land and sea. Environmental gradients. Ecotones and transitions. Plant diversity in coastal, rocky and lagoon coastal environments. Major features and distribution throughout the world. Plant communites in Italy and Europe (Mediterranean and Atlantic). Analyses of main plant communities and habitats (European Directive 92/43/EEC (Habitat Directive) present in Italy. Conservation problems. Sampling tecniques and data analyses. Ecologia e diversità faunistica delle coste sabbiose, delle lagune costiere e delle coste roccioseThe animal communities of coastal dunes and their ecological relations with plants. Interactions between the animal communities of coastal dunes and Mediterranean evergreen scrub. The fauna of coastal wetlands and their importance as sites of bird nesting and winter refuges. The Ramsar Convention: conservation and management issues in wetlands. Stranding and recovery of marine vertebrates (whales, dolphins and sea turtles). Nesting of sea turtles and their conservation. Coexistence of marine and land animals in the intertidal zone. Fauna of tide pools. Sea bird nesting on rock cliffs: conservation and management issues.Conservation and management of coastal ecosystemsConservation of coastal ecoystems. Disturbance factors. The role of disturbance on coastal ecosystems. Response to disturbance. Endangered species. Alien species. Bioindicators. Environmental quality and conservation status. Conservation and Management.
( reference books)
Acosta A. & Ercole S. 2017. Gli habitat delle coste sabbiose italiane: ecologia e problematiche di conservazione. Quaderni ISPRA (Istituto Superiore per la Protezione e la Ricerca Ambientale 215/2015.Ruffo, S. (a cura di). (2002). Dune e spiagge sabbiose. Ambienti fra terra e mare. Quaderni Habitat, 4. Ministero dell’Ambiente e della Tutela del Territorio. Scaricabile dal sito del Ministero.
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20402509-3 -
MODULO 3
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ACOSTA ALICIA TERESA ROSARIO
( syllabus)
Ecology and plant diversity of coastal ecosystems Coastal ecosystems, the transition between land and sea. Environmental gradients. Ecotones and transitions. Plant diversity in coastal, rocky and lagoon coastal environments. Major features and distribution throughout the world. Plant communites in Italy and Europe (Mediterranean and Atlantic). Analyses of main plant communities and habitats (European Directive 92/43/EEC (Habitat Directive) present in Italy. Conservation problems. Sampling tecniques and data analyses. Ecologia e diversità faunistica delle coste sabbiose, delle lagune costiere e delle coste roccioseThe animal communities of coastal dunes and their ecological relations with plants. Interactions between the animal communities of coastal dunes and Mediterranean evergreen scrub. The fauna of coastal wetlands and their importance as sites of bird nesting and winter refuges. The Ramsar Convention: conservation and management issues in wetlands. Stranding and recovery of marine vertebrates (whales, dolphins and sea turtles). Nesting of sea turtles and their conservation. Coexistence of marine and land animals in the intertidal zone. Fauna of tide pools. Sea bird nesting on rock cliffs: conservation and management issues.Conservation and management of coastal ecosystemsConservation of coastal ecoystems. Disturbance factors. The role of disturbance on coastal ecosystems. Response to disturbance. Endangered species. Alien species. Bioindicators. Environmental quality and conservation status. Conservation and Management.
( reference books)
Acosta A. & Ercole S. 2017. Gli habitat delle coste sabbiose italiane: ecologia e problematiche di conservazione. Quaderni ISPRA (Istituto Superiore per la Protezione e la Ricerca Ambientale 215/2015.Ruffo, S. (a cura di). (2002). Dune e spiagge sabbiose. Ambienti fra terra e mare. Quaderni Habitat, 4. Ministero dell’Ambiente e della Tutela del Territorio. Scaricabile dal sito del Ministero.
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20402509-2 -
MODULO 2
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CARPANETO GIUSEPPE
( syllabus)
ANIMAL DIVERSITY AND ECOLOGY OF COASTAL ECOSYSTEMSTHE ANIMAL COMMUNITIES OF COASTAL DUNES AND THEIR ECOLOGICAL RELATIONS WITH PLANTS. INTERACTIONS BETWEEN THE ANIMAL COMMUNITIES OF COASTAL DUNES AND MEDITERRANEAN EVERGREEN SCRUB. THE FAUNA OF COASTAL WETLANDS AND THEIR IMPORTANCE AS SITES OF BIRD NESTING AND WINTER REFUGES. THE RAMSAR CONVENTION: CONSERVATION AND MANAGEMENT ISSUES IN WETLANDS. STRANDING AND RECOVERY OF MARINE VERTEBRATES (WHALES, DOLPHINS AND SEA TURTLES). NESTING OF SEA TURTLES AND THEIR CONSERVATION. COEXISTENCE OF MARINE AND LAND ANIMALS IN THE INTERTIDAL ZONE. FAUNA OF TIDE POOLS. SEA BIRD NESTING ON ROCK CLIFFS: CONSERVATION AND MANAGEMENT ISSUES.CONSERVATION AND MANAGEMENT OF COASTAL ECOSYSTEMSCONSERVATION OF COASTAL ECOSYSTEMS. DISTURBANCE FACTORS. THE ROLE OF DISTURBANCE ON COASTAL ECOSYSTEMS. HUMAN IMPACT, URBANIZATION AND MARINE LITTER (MARINE DEBRIS). RESPONSE TO DISTURBANCE. ENDANGERED SPECIES. ALIEN SPECIES. BIOINDICATORS. ENVIRONMENTAL QUALITY AND CONSERVATION STATUS. PRINCIPLES OF COASTAL MANAGEMENT.
( reference books)
RUFFO, S. (A CURA DI). (2002). DUNE E SPIAGGE SABBIOSE. AMBIENTI FRA TERRA E MARE. QUADERNI HABITAT, 4. MINISTERO DELL’AMBIENTE E DELLA TUTELA DEL TERRITORIO. SCARICABILE DAL SITO DEL MINISTERO.RUFFO, S. (A CURA DI). (2004). COSTE MARINE ROCCIOSE. LA VITA FRA ROCCE E SALSEDINE. AMBIENTI FRA TERRA E MARE. QUADERNI HABITAT, 7. MINISTERO DELL’AMBIENTE E DELLA TUTELA DEL TERRITORIO. SCARICABILE DAL SITO DEL MINISTERO.MCLACHLAN A., BROWN A.C., 2006. THE ECOLOGY OF SANDY SHORES. ACADEMIC PRESS, BURLINGTON, MA, USA.MARTINEZ, M.L. & PSUTY N.P. (EDS.) . (2004). COASTAL DUNES. ECOLOGY AND CONSERVATION. ECOLOGICAL STUDIES 171. SPRINGER-VERLAG.MARTINEZ M.I., HESP P. &. GALLEGO-FERNANDEZ J.B. 2013. RESTORATION OF COASTAL DUNES. ELSEVIER, NETHERLANDS.PACKHAM, J.R. & WILLIS, A.J. (1997). ECOLOGY OF DUNES, SALT MARSH AND SHINGLE. CHAPMAN & HALL. LONDON.POWER POINT FILES AND OTHER ELECTRONIC FILES, GIVEN BY THE PROFESSORThe professor receives every day from 15:00 to 18:30 by appointment via email: giuseppe.carpaneto@uniroma3.it
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20402510 -
INLAND AND MARINE WATER ECOLOGY
(objectives)
The main aim is to provide a solid knowledge on both abiotic and biotic features of ecological processes considered to be common in aquatic systems. A second goal is to develop identification ability of the main aquatic species of Mediterranean systems by a comparative analysis of the different life strategies and colonization patterns of extreme habitats, and the possibility to origin more and more structured multi-species assemblanges to start ecological series. During the course, it will be introduced methods and tools to perform aquatic investigations. Students will receive information to design and execute sampling sessions on plankton, benthos e nekton, to analyze and show findings. Finally students will learn to develop applicative aspects of work in diverse issues in the field of aquatic biomonitoring and hydrobiology.
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20402511 -
LABORATORY OF ELECTRON AND ION BEAM MICROSCOPY APPLIED TO BIOLOGICAL SYSTEMS
(objectives)
THE COURSE AIMS TO PROVIDE THE STUDENT WITH BASIC KNOWLEDGE AND TECHNICAL SKILLS IN ORDER TO APPROACH THE STUDY OF BIOLOGICAL SPECIMENS BY ULTRASTRUCTURAL MORFOLOGICAL METHODOLOGIES.
MAIN OBJECTIVES INCLUDE LEARNING AND APPLYING:1) BASIC PRINCIPLES OF ELECTRON MICROSCOPY; 2) METHODOLOGIES OF SAMPLE PREPARATION (PROKARYOTES AND EUKARYOTES) FOR ULTRASTRUCTURAL ANALYSIS; 3) USAGE OF DEVICES FOR ULTRASTRUCTURAL INVESTIGATION (SCNANING, TRANSMISSION AND FOCUSING ION BEAM MICROSCOPY); 3) SOFTWARE FOR MICROSCOPIC IMAGE CAPTURING AND PROCESSING 4) QUALITATIVE INTERPRETATION AND QUANTITATIVE ANALYSIS OF ULTRASTRUCTURAL DATA.
THE STUDENT WILL BE ABLE TO AUTONOMOUSLY CHOOSE AND ADJUST EXPERIMENTAL PROTOCOLS, SUITED TO DIFFERENT SAMPLE TYPES AND TO THE OBJECTIVE OF THE STUDY. KNOWLEDGE ACQUIRED DURING THE COURSE WILL ALLOW THE STUDENT TO CRITICALLY ANALYZE MORPHOLOGICAL RESULTS IN A FUNCTIONAL CONTEXT.
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20402511-1 -
MODULO 1
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Derived from
20402511 LABORATORIO DI MICROSCOPIA ELETTRONICA E IONICA PER LO STUDIO DI SISTEMI BIOLOGICI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
PRINCIPLES OF ELECTRONIC MICROSCOPY. TRANSMISSION MICROSCOPY (TEM). SCANNING ELECTRON MICROSCOPY (SEM). ION BEAM MICROSCOPY (FIB / SEM). STUDY OF THE ATOMIC COMPOSITION BY EDAX PROBE.PREPARATION OF BIOLOGICAL SAMPLES FOR MORPHOLOGICAL ANALYSIS: GENERAL PRINCIPLES. COLLECTION OF SAMPLES. FIXATION METHODS: IMMERSION, PERFUSION MODES. COAGULANT AND NON-COAGULANT FIXATIVES. POST-FIXATION METHODS FOR ELECTRONIC MICROSCOPY. CRIO-FIXATION TECHNIQUES.TEM: DEHYDRATION AND INCLUSION IN ACRYLIC AND EPOXY RESINS. ULTRAMICROTOMY: PREPARATION OF GLASS BLADES, SECTION OF THIN AND ULTRA THIN SLICES. USE OF DIAMOND BLADES. COLLECTION OF SECTIONS ON GRIDS AND CONTRAST. USE OF TEM: BEAM ALIGNMENT, SAMPLE POSITIONING, FOCUSING, CAPTURE OF IMAGES.SEM: DEHYDRATION METHODS AND USE OF THE CRITICAL POINT DRYER. SPECIMEN MOUNTING AND METALLIZATION OF SAMPLES BY SPUTTER-COATER. USE OF SEM IN THE DETECTION OF SECONDARY AND BACK-SCATTERED ELECTRONICS. IMAGING AND MORPHOMETRIC ANALYSIS.FIB / SEM: USE OF THE FIB / SEM MICROSCOPE ON SAMPLES PROVIDED FOR TEM AND SEM. MILLING TECHNIQUES, PLATINUM DEPOSITION. PREPARATION OF TEM SLICES AND CROSS SECTIONS. ACQUISITION OF SERIAL IMAGES AND THEIR PROCESSING.PRE-AND POST-EMBEDDING IMMUNOLOCALIZATION: IMMUNOENZYMATIC METHODS AND USE OF ANTIBODIES CONJUGATED TO COLLOIDAL NANOPARTICLES.
( reference books)
MATERIAL AND PPT SLIDES SUPPLIED BY THE PROFESSORS.Office Hours: every day of the week by appointment by email (andrea.digiulio@uniroma3.it)
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20402511-2 -
MODULO 2
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Derived from
20402511 LABORATORIO DI MICROSCOPIA ELETTRONICA E IONICA PER LO STUDIO DI SISTEMI BIOLOGICI in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
The course aims to provide the student with basic knowledge and technical skills in order to approach the study of biological specimens by ultrastructural morfological methodologies. The student will be able to autonomously choose and adjust experimental protocols, suited to different sample types and to the objective of the study. Knowledge acquired during the course will allow the student to critically analyze morphological results in a functional context.Upon completion of this course, the student will acquire:1) Knowledge of basic principles of electron microscopy; 2) Knowledge and competence on methodologies of sample (prokaryotes and eukaryotes) preparation for ultrastructural analysis; 3) Basic competence for utilization of devices for ultrastructural investigation (scnaning, transmission and focusing ion beam microscopy); 3) Competence on the use of appropriate software for microscopic image capturing and processing;4) Basic competence on qualitative interpretation and quantitative analysis of ultrastructural data.Course programmeElectron microscopy principles. Trasmission electron microscopy (tem). Scanning electron microscopy (sem). Focussing ion beam microscopy (fib/sem). Use of edax probe for the study of atomic composition.Biological sample preparation for morphological analysis: general principles. Sample harvesting. Fixing methods: immersion and perfusion. Fixative solutions. Post-fixation methods for electron microscopy. Cryofixation techniques.Tem: dehydration and embedding in acrylic ed epoxy resins. Ultramicrotomy: glass knives preparing, thin and ultrathin sectioning. Usage of diamond knives. Section harvesting on grids and contrasting methods. Use of tem: lens setting and alignment, sample positioning, focussing, image capturing.Sem: dehydration methods and use of critical point dryer. Mounting and metallizing of samples, by sputter-coater. Use of sem in the modalities of secondary and back-scattered electrons detection. Image capturing and morphometric analyses. Fib/sem: use of fib/sem on samples prepared for tem e sem. Milling and deposition techniques. Tem lamellae preparation. Cross sections. Serial images acquisition and processing.Immunolocalization by pre- and post-embedding methods: immunoenzymatic procedures and usage of antibodies conjugated to colloidal gold nanoparticles.
( reference books)
Booklets of lectures and lab sessions will be provided.The instructor will be available every working day, from 10 am to 1 pm, even to provide further bibliographic references, on appointment by email:sandra.moreno@uniroma3.it
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3
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BIO/06
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8
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20
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20402512 -
METHODS AND TECHNIQUES IN VEGETATION ECOLOGY
(objectives)
MAJOR GOALS OF THIS COURSE ARE THE ACQUISITION OF KNOWLEDGE ON THE MAIN METHODS AND TECHNIQUES FOR THE STUDY OF VEGETATION:
- ACQUIRE THE MASTERY OF THE STUDY METHODS OF VEGETATION;
- INCREASE THE ABILITY TO INTERPRETING THE NATURAL AND ARTIFICIAL PHENOMENA WITH SPECIAL ATTENTION TO THE MECHANISMS OF TRANSFORMATION OF PLANT LANDSCAPES;
- DEVELOP THE CAPACITY TO APPLICATION OF MOST APPROPRIATE METHODS FOR MONITORING OF THE PLANT COMMUNITIES FOR CONSERVATION AND MANAGEMENT PURPOSES;
- INCREASE THE POSSIBILITIES OF INTERPRETATION OF EXPERIMENTAL DATA AND UNDERSTANDING OF SCIENTIFIC ARTICLES IN THE FIELDS OF PLANT ECOLOGY, GEOBOTANY AND APPLIED ECOLOGY.
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20402512-1 -
MODULO 1
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CUTINI MAURIZIO
( syllabus)
DEFINITION AND PROPERTIES OF PLANT COMMUNITIES. STRUCTURAL AND PHYSIOGNOMICAL CHARACTER OF VEGETATION. DISCRETE AND CONCRETE COMMUNITIES. ZONES AND BANDS OF VEGETATION. CONCEPT OF ZONALITY, AZONALITY AND EXTRAZONALITY. VEGETATION DYNAMICS AND POTENTIAL NATURAL VEGETATION.GROWTH FORMS AND PLANT TRAITS. ANALYSES OF THE FUNCTIONAL DIVERSITY AND GRIME’S STRATEGIES (C-S-R). DIVERSITY ANALYSES: GENERAL CONCEPT, ALGORITHMS CALCULATION, RAREFACTION CURVE, ABUNDANCE/DOMINANCE RANK, RE’NY CURVES. SAMPLING METHODS OF THE PLANT COMMUNITIES (RANDOM AND SYSTEMATIC SAMPLING). SIZE AND DISTRIBUTION OF SAMPLES. ABUNDANCE MEASURES. ORIGIN AND DEVELOPMENT OF THE PHYTOSOCIOLOGY AND SYMPHYTOSOCIOLOGY. GENERAL CONCEPT OF THE VEGETATION MAPPING. VEGETATION AND HABITAT INTERPRETATION (EUNIS AND ALL. I OF THE 92/43/CEE DIRECTIVE. HABITATS MONITORING, EUROPEAN AND NATIONAL RED LISTS OF HABITAT. MULTITEMPORAL ANALYSIS, ANTHROPIC IMPACT AND EFFECT OF VEGETATION.
( reference books)
BRECKLE S.W., 2002. Walter’s Vegetation of the Earth.Springer. VAN DER MAAREL E. (Ed.), 2005. Vegetation Ecology. Blackwell Publishing.
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2
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BIO/03
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16
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20402512-2 -
MODULO 2
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ACOSTA ALICIA TERESA ROSARIO
( syllabus)
DEFINITION OF THE GOALS OF THE COURSE AND RELATIONSHIP WITH OTHER ENVIRONMENTAL DISCIPLINE. DEFINITION AND PROPERTIES OF PLANT COMMUNITIES. STRUCTURAL AND PHYSIOGNOMICAL CHARACTER OF VEGETATION. DISCRETE AND CONCRETE COMMUNITIES. ZONES AND BANDS OF VEGETATION. CONCEPT OF ZONALITY, AZONALITY AND EXTRAZONALITY. VEGETATION DYNAMICS AND POTENTIAL NATURAL VEGETATION.DIVERSITY ANALYSES: GENERAL CONCEPT, ALGORITHMS CALCULATION, RAREFACTION CURVE, ABUNDANCE/DOMINANCE RANK, RE’NY CURVES. SAMPLING METHODS OF THE PLANT COMMUNITIES (RANDOM AND SYSTEMATIC SAMPLING). SIZE AND DISTRIBUTION OF SAMPLES. ABUNDANCE MEASURES. GROWTH FORMS AND PLANT TRAITS. ANALYSES OF THE FUNCTIONAL DIVERSITY.ORIGIN AND DEVELOPMENT OF THE PHYTOSOCIOLOGY AND SYMPHYTOSOCIOLOGY. VEGETATION AND HABITAT INTERPRETATION SENSU 92/43/CEE DIRECTIVE. GENERAL CONCEPT OF THE VEGETATION MAPPING. PRACTICAL ACTIVITY: FIELD ACTIVITIES, VEGETATION SAMPLING, STATISTICAL ANALYSIS.
( reference books)
KENT M. & COKER P., 1992. VEGETATION DESCRIPTION AND ANALYSES. JOHN WILEY AND SONS.MUELLER-DUMBOIS D. & ELLENBERG H., 1974. AIMS AND METHODS OF VEGETATION SCIENCE. JOHN WILEY AND SONS.PEDROTTI F., 2004. CARTOGRAFIA GEOBOTANICA. PITAGORA ED.VAN DER MAAREL E. (ED.), 2005. VEGETATION ECOLOGY. BLACKWELL PUBLISH.
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2
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BIO/03
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16
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20402512-3 -
MODULO 3
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CUTINI MAURIZIO
( syllabus)
DEFINITION AND PROPERTIES OF PLANT COMMUNITIES. STRUCTURAL AND PHYSIOGNOMICAL CHARACTER OF VEGETATION. DISCRETE AND CONCRETE COMMUNITIES. ZONES AND BANDS OF VEGETATION. CONCEPT OF ZONALITY, AZONALITY AND EXTRAZONALITY. VEGETATION DYNAMICS AND POTENTIAL NATURAL VEGETATION.GROWTH FORMS AND PLANT TRAITS. ANALYSES OF THE FUNCTIONAL DIVERSITY AND GRIME’S STRATEGIES (C-S-R). DIVERSITY ANALYSES: GENERAL CONCEPT, ALGORITHMS CALCULATION, RAREFACTION CURVE, ABUNDANCE/DOMINANCE RANK, RE’NY CURVES. SAMPLING METHODS OF THE PLANT COMMUNITIES (RANDOM AND SYSTEMATIC SAMPLING). SIZE AND DISTRIBUTION OF SAMPLES. ABUNDANCE MEASURES. ORIGIN AND DEVELOPMENT OF THE PHYTOSOCIOLOGY AND SYMPHYTOSOCIOLOGY. GENERAL CONCEPT OF THE VEGETATION MAPPING. VEGETATION AND HABITAT INTERPRETATION (EUNIS AND ALL. I OF THE 92/43/CEE DIRECTIVE. HABITATS MONITORING, EUROPEAN AND NATIONAL RED LISTS OF HABITAT. MULTITEMPORAL ANALYSIS, ANTHROPIC IMPACT AND EFFECT OF VEGETATION.
( reference books)
BRECKLE S.W., 2002. Walter’s Vegetation of the Earth.Springer. VAN DER MAAREL E. (Ed.), 2005. Vegetation Ecology. Blackwell Publishing.
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2
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BIO/03
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20
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20402518 -
TOXICOLOGY
(objectives)
THE COURSE AIMS TO PROVIDE PRECISE INFORMATION ON THE TOXICODYNAMIC AND TOXICOKINETIC MECHANISMS THAT ARE BEHIND THE TOXIC EFFECTS PRODUCED BY DIFFERENT NATURAL AND SYNTHESISED XENOBIOTICS, AND ON THE PROCESS OF EVALUATION OF TOXICOLOGICAL RISKS.
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Derived from
20402518 TOSSICOLOGIA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 N0 TREZZA VIVIANA
( syllabus)
GENERAL PRINCIPLES. AIMS AND FIELDS OF TOXICOLOGY. CLASSIFICATION OF TOXIC AGENTS. MECHANISMS OF MOLECULAR AND CELL TOXICITY. RISK EVALUATION. TOXICOKINETICS: ABSORPTION, DISTRIBUTION, METABOLISM AND EXCRETION OF TOXIC AGENTS. ADVERSE DRUG REACTIONS, DRUG INTERACTIONS AND PRINCIPLES OF PHARMACOVIGILANCE. ANTIDOTES AND THEIR CLASSIFICATION. EXPERIMENTAL EVALUATION OF TOXIC EFFECTS IN IN VITRO AND IN VIVO MODELS AND DATA EXTRAPOLATION. SYSTEM AND ORGAN TOXICOLOGY. DEVELOPMENTAL AND FEEDING TOXICOLOGY. CLASSES OF TOXIC COMPOUNDS: METALS, PHYTODRUGS, NATURAL TOXINS. PSYCHOACTIVE AND DOPING DRUGS.
( reference books)
CASARETT & DOULL'S “ESSENTIALS OF TOXICOLOGY”, SECOND EDITION, MCGRAW HILL
Power-point files of the lessons will be provided
To get an appointment with the Professor, email to: viviana.trezza@uniroma3.it
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6
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BIO/14
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48
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20402519 -
APPLIED CELLULAR NEUROBIOLOGY
(objectives)
THE AIMS OF THIS COURSE ARE: TO GAIN KNOWLEDGE OF THE SPECIALIZED CELLULAR ARCHITECTURE OF GLIA AND NEURONS; TO DEEPEN THE KNOWLEDGE OF CELLULAR AND MOLECULAR MECHANISMS INVOLVED IN CELL COMMUNICATION, SPECIAL EMPHASIS WILL BE GIVEN TO NITRIC OXIDE-REGULATED PATHWAYS. IN THIS COURSE STUDENTS CAN USE STATE OF THE ART EQUIPMENT AND LEARN CURRENT TECHNIQUES EMPLOYED IN CELL BIOLOGY RESEARCH LABS. MOREOVER STUDENTS ARE ALLOWED TO CRITICALLY DISCUSS THE SCIENTIFIC LITERATURE IN THE FIELD OF CELL BIOLOGY.
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20402519-1 -
MODULO 1
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Derived from
20402519 NEUROBIOLOGIA CELLULARE APPLICATA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology.Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity. Topics: The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA” – THIRD EDITION – 2012 OXFORD UNIVERSITY PRESSSlides in PDF format of all the lectures will be provided by the teacher.The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it
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4
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BIO/06
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32
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20402519-2 -
MODULO 2
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Derived from
20402519 NEUROBIOLOGIA CELLULARE APPLICATA in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology.Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity. Topics: The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA” – THIRD EDITION – 2012 OXFORD UNIVERSITY PRESS SLIDES AND SCIENTIFIC PAPERS PROVIDED BY THE TEACHER.
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2
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BIO/06
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20
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20402520 -
PLANT BIOTECHNOLOGY FOR THE GENETIC IMPROVEMENT OF FOOD CROPS
(objectives)
THIS COURSE WILL PROVIDE STUDENTS WITH AN IN-DEPTH KNOWLEDGE OF PLANT TRANSFORMATION TECHNIQUES AND PLANT BIOTECHNOLOGY APPLICATIONS WITHIN AGRICULTURE AND PRODUCTION OF NEW MOLECULES. THIS COURSE WILL ALSO GIVE EDUCATION ON EMERGING TECHNOLOGIES, SUCH AS TRANSCRIPTOMICS, PROTEOMICS, METABOLOMICS, AND WILL DESCRIBE THEIR IMPORTANCE IN PLANT BIOTECHNOLOGY RESEARCH. ANOTHER IMPORTANT AIM OF THE COURSE IS TO PREPARE STUDENTS TO CONDUCT LABORATORY WORK AND RESEARCH IN THE SECTORS OF PLANT BIOTECHNOLOGY AND FOOD INDUSTRY, AS WELL AS TO CRITICALLY ANALYZE THE SCIENTIFIC INFORMATION
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20402520-2 -
MODULO 2
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Derived from
20402520 BIOTECNOLOGIE PER IL MIGLIORAMENTO GENETICO DELLE PIANTE in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
LABORATORY ACTIVITY: TRANSFORMATION OF ARABIDOPSIS PLANTS; ANALYSIS OF TRANSGENIC PLANTS BY PCR, RT-PCR, WESTERN BLOT AND ENZYME ACTIVITY ASSAYS; ANALYSIS OF REPORTER GENES IN TRANSGENIC PLANTS; ANALYSIS OF FOOD MATERIAL FOR THE PRESENCE OF GENETICALLY MODIFIED MATERIAL.
( reference books)
-Recommended textbooks:1. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF PLANTS (2000) BUCHANAN B.B, GRUISSEM W., JONES R.L., AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MARYLAND.2. BIOLOGIA CELLULARE E BIOTECNOLOGIE VEGETALI (2011) COZZOLINO S., DI SANSEBASTIANO G.P., FORNI C., GENRE A., LANFRANCO L., MICCHELI A., PASQUA G., TRAINOTTI L., VALLETTA A.3. HANDBOOK OF PLANT BIOYECHNOLOGY (2004) CHRISTOU P., KLEE H., HOBOKEN, (N.J.), WILEY.4. PLANT BIOTECHNOLOGY AND TRANSGENIC PLANTS (2002) OKSMAN-CALDENTEY K.-M., BARZ W.H., NEW YORK, DEKER.-Scientific articles published in international journals (pdf files will be provided).-Lecture notes will be provided.
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1,5
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BIO/04
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15
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20402520-1 -
MODULO 1
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Derived from
20402520 BIOTECNOLOGIE PER IL MIGLIORAMENTO GENETICO DELLE PIANTE in BIOLOGIA PER LA RICERCA MOLECOLARE, CELLULARE E FISIOPATOLOGICA (DM 270) LM-6 (docente da definire)
( syllabus)
THREE ARE THE MAIN ARGUMENTS OF THE COURSE: GENE TRANSFER TECHNIQUES; APPLICATIONS OF PLANT BIOTECHNOLOGY; ANALYSIS OF RISKS AND BENEFITS OF PLANT GENETIC MANIPULATION.IN DETAIL, DURING THE COURSE THE FOLLOWING ARGUMENTS ARE DISCUSSED:TECHNIQUES FOR GENE TRANSFER INTO THE NUCLEAR AND CHLOROPLASTIC GENOME; TECHNIQUES FOR IN VITRO CULTURE OF PLANT CELLS; USE OF SELECTABLE GENE MARKERS AND REPORTER GENES; STRATEGIES TO REMOVE SELECTABLE GENE MARKERS FROM TRANSGENIC PLANTS; INDUCIBLE EXPRESSION OF TRANSGENES IN PLANTS; TRANSIENT EXPRESSION OF GENES IN PLANTS; GENE SILENCING IN PLANTS; ‘GENE TARGETING’ IN PLANTS; GENOME EDITINGIN IN PLANTS; PLANTS WITH IMPROVED NUTRITIONAL VALUE; PRODUCTION OF ANTIBODIES AND VACCINES IN TRANSGENIC PLANTS; BIOTECHNOLOGICAL STRATEGIES TO OBTAIN PLANTS RESISTANT TO HERBICIDES, PATHOGENS AND ENVIRONMENTAL STRESSES; PLANTS WITH INCREASED PHOTOSYNTHETIC RATE; BIOSAFETY AND BIOETHICAL ISSUES RELATED TO PLANT BIOTECHNOLOGY; EMERGING TECHNOLOGIES AND PLANT BIOTECHNOLOGY.
( reference books)
Recommended textbooks:1. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF PLANTS (2000) BUCHANAN B.B, GRUISSEM W., JONES R.L., AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MARYLAND.2. BIOLOGIA CELLULARE E BIOTECNOLOGIE VEGETALI (2011) COZZOLINO S., DI SANSEBASTIANO G.P., FORNI C., GENRE A., LANFRANCO L., MICCHELI A., PASQUA G., TRAINOTTI L., VALLETTA A.3. HANDBOOK OF PLANT BIOYECHNOLOGY (2004) CHRISTOU P., KLEE H., HOBOKEN, (N.J.), WILEY.4. PLANT BIOTECHNOLOGY AND TRANSGENIC PLANTS (2002) OKSMAN-CALDENTEY K.-M., BARZ W.H., NEW YORK, DEKER.-Scientific articles published in international journals (pdf files will be provided).-Lecture notes will be provided.
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4,5
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BIO/04
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36
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20402521 -
MOLECULAR ENDOCRINOLOGY
(objectives)
ENDOCRINOLOGY IS USUALLY TAUGHT AT THE END OF STANDARD PHYSIOLOGY COURSES, BECAUSE THE ENDOCRINE SYSTEM REGULATES ALL THE OTHER ORGAN SYSTEMS. IN THIS RESPECT, LEARNING ABOUT ENDOCRINE SYSTEM HAS THE ADDED CHALLENGE OF INTEGRATING STUDENT KNOWLEDGE OF HORMONES AND THEIR ACTIONS WITH THE GENERAL FUNCTION OF A SPECIFIC ORGAN SYSTEM. THE MAIN OBJECTIVES OF THIS COURSE ARE TO ALLOW THE STUDENT TO GAIN A FOUNDATION OF KNOWLEDGE ABOUT THE ENDOCRINE SYSTEM AND INTEGRATIVE PHYSIOLOGY, TO BECOME REFINED IN THE CRITICAL ANALYSIS OF SCIENTIFIC PAPERS RELATED TO ENDOCRINE PHYSIOLOGY, TO DEVELOP PROBLEM SOLVING AND PUBLIC SPEAKING TECHNIQUES.
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20402522 -
DEVELOPMENTAL NEUROBIOLOGY
(objectives)
THE MAIN GOAL IS TO PROVIDE ADVANCED KNOWLEDGE IN DEVELOPMENTAL BIOLOGY, WITH SPECIAL REFERENCE TO THE NERVOUS SYSTEM. THE CELLULAR AND MOLECULAR MECHANISMS UNDERLYING NERVOUS TISSUE DIFFERENTIATION AND MORPHOGENESIS WILL BE ADDRESSED IN DIFFERENT IN VIVO MODELS. LIFE-LONG FUNCTIONAL IMPLICATIONS OF NEUROGENESIS AND PLASTICITY WILL BE EMPHASIZED. THE STUDENT WILL BE ABLE TO: 1) DESCRIBE DEVELOPMENTAL PROCESSES OF INVERTEBRATE AND VERTEBRATE NERVOUS SYSTEM; 2) RECOGNISE MOLECULAR PATHWAYS DIRECTING STEM CELLS TOWARDS NEURONAL/GLIAL CELL DIFFERENTIATION; 3) CORRELATE ONTOGENETIC ABNORMALITIES WITH SPECIFIC NEUROLOGICAL DISTURBANCES, INCLUDING INFANTILE DISEASES AND AGE-RELATED NEURODEGENERATION.
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20402522-1 -
MODULO 1
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5
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BIO/06
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40
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20402522-2 -
MODULO 2
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1
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BIO/06
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10
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20402523 -
MOLECULAR AND CELLULAR PHYSIOLOGY
(objectives)
ON THE BASIS OF THE MOST RECENT SCIENTIFIC LITERATURE, THE COURSE IS AIMED TO ALLOW THE STUDENTS TO UNDERSTAND SOME ASPECTS OF THE REGULATION OF CELLULAR FUNCTIONS
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20402523-1 -
MODULO 1
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5
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BIO/09
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40
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20402523-2 -
MODULO 2
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1
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BIO/09
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10
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Università Roma Tre