Optional group:
CARATTERIZZANTI - (show)
![Info](/images/iconai.png) |
12
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20410207 -
Biochimica della nutrizione
(objectives)
The Nutrition Biochemistry course is aimed at providing to the students the basic knowledge to understand the molecular mechanisms by which the human organism uses macro- and micro-nutrients to produce the energy needed to maintain the order which characterizes every living being. In particular, the objectives of the course are the comprehension of the pathways through which the different groups of nutrients (carbohydrates, lipids, proteins, vitamins and minerals) and other substances of natural and non-natural origin (ethanol, stimulant beverages, xenobiotics) are assimilated and transformed in the human body, and how they influence its metabolic status. Finally, one of the aims is also that of illustrating the production of reactive oxygen species linked to metabolism and the protective role exerted by antioxidant compounds of dietary origin
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6
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BIO/10
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48
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Core compulsory activities
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ITA |
20410486 -
Laboratorio di neurobiologia cellulare
(objectives)
The aims of this course are: 1)to gain knowledge of the specialized cellular architecture of glia and neurons; 2)to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
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Derived from
20410486 Laboratorio di neurobiologia cellulare in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 PERSICHINI TIZIANA
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology. Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity. Topics: The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA” – THIRD EDITION – 2012 OXFORD UNIVERSITY PRESS
Slides in PDF format of all the lectures will be provided by the teacher.
The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it
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6
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BIO/06
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32
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20
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-
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Core compulsory activities
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ITA |
20402369 -
MICROBIAL GENETICS
(objectives)
The course of Microbial Genetics is organized to provide the student with: (i) adequate knowledge and understanding of advanced methodologies in bacterial genetics, appropriate methodologies and communication skills for the study of genotype-phenotype relationships in bacteria; (ii) adequate applied knowledge for the evaluation of experimental approaches, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgments).
The aims of the course are: (i) the acquisition of advanced knowledge on the evolution and functioning of bacterial genomes and extrachromosomal genetic elements, and on the approaches that can be used to investigate these topics; (ii) the acquisition of critical knowledge for the choice and design of experimental protocols; (iii) the acquisition of knowledge and skills for retrieving relevant scientific literature from on-line databases and for critically reviewing it.
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6
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BIO/18
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40
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10
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-
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-
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Core compulsory activities
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ITA |
20410314 -
GENETICA DEI SISTEMI COMPLESSI
(objectives)
The course aim to provide a wider and deeper outlook of genetics topics not addressed in the course of genetics. Special attention will be paied to the role of human genome (and epigenome) in the regulation of complex biological processes and in the individual response to endogenous and exogenous factors.
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Derived from
20410314 GENETICA DEI SISTEMI COMPLESSI in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 BERARDINELLI FRANCESCO
( syllabus)
- GENOME (ORGANIZATION, STRUCTURAL GENOMICS, COMPARATIVE GENOMICS AND FUNCTUNIAL GENOMICS). EPIGENOME (METHYLATION, HISTONE CODE, CHROMATIN 3D ARCHITECTURE, NON-CODING RNA). EXAMPLES OF EPIGENETIC REGULATION: CELL DIFFERENTIATION AND GENETIC MECHANISMS OF SEX DETERMINATION (HOW DOES SEX IS GENETICALLY DETERMINED, INACTIVATION OF X CHROMOSOME, SEX-DEPENDENT PHONOTYPIC EFFECTS). - CELL CYCLE REGULATION, SENESCENCE AND APOPTOSIS. GENETIC OF AGING (GENETIC THEORIES OF AGING, GERONTOGENES AND GENES INVOLVED IN LONGEVITY, AGING IN ANIMAL MODELS, MOLECULAR AND CELLULAR AGING). CANCER GENETICS (ONCOGENES, ONCO-SUPPRESSOR GENES, CHROMOSOMAL REARRANGEMENTS INVOLVED IN CANCER, THE CANCER STEM CELLS THEORY). INDIVIDUAL GENETIC RESPONSE AND PHARMACOGENETICS.
( reference books)
- SCIENTIFIC ARTICLES - TOM STRACHAN and ANDREW READ, HUMAN MOLECULAR GENETICS, 5th Edition, CRC PRESS (Taylor & Francis Group)
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6
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BIO/18
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40
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10
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Core compulsory activities
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ITA |
20401821 -
IMMUNOLOGY
(objectives)
Cellular and molecular mechanisms involved in the immune response with a particular interest for those implicated in the response against pathogens.
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6
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MED/04
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48
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-
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-
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Core compulsory activities
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ITA |
20410212 -
Genetica umana applicata
(objectives)
The main aims of the course are: 1. To consolidate knowledge in Human Genetics acquired during previous basic courses, with particular reference to biological mechanisms involved in genetic diseases 2. To carry on theorical notions on a practical point of view by the knoledge of diagnostic laboratory methods and strategies
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6
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BIO/18
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48
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-
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Core compulsory activities
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ITA |
20410278 -
MICROBIOLOGIA AMBIENTALE
(objectives)
The main aims of the course are: 1. acquire knowledge of the fundamental role of microorganisms in ecosystems and of the factors affecting their distribution and interactions with other organisms: - metabolic and functional biodiversity, structure and dynamics of microbial communities - bacteria and archea taxonomic groups 2. knowledge of traditional, molecular and cultivation-independent methods for identification/typing and analysis of microbial populations 3. evaluation of the multiple potential applications of environmental microorganisms also as bioindicators 4. acquisition of critical skills by reading scientific articles.
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6
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BIO/19
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44
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-
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5
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Core compulsory activities
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ITA |
20410213 -
Nutrienti di origine vegetale
(objectives)
The course "Nutrients of plant origin" is part of the Plant Physiology training activities of the Master of Science in Biology for Molecular, Cellular and Patho-physiological Research. This course provides basic knowledge relating to the qualitative and quantitative composition and nutritional properties of plant-based foods and their derived processed counterparts. In particular, the course provides 1) an overview of the plants used for human nutrition and their use for the preparation of food products, with an outline of the influence of processing processes on the nutritional properties; 2) an in-depth picture of the main nutrients, aromas and other substances useful for human nutrition present in food of plant origin; 3) a summary of the anti-nutritional factors, allergens and toxic compounds present in some foods of plant origin.
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Derived from
20410213 Nutrienti di origine vegetale in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 CONA ALESSANDRA
( syllabus)
Climate change and food security. Plant varieties and phenotypic plasticity. Effect of environmental conditions on primary and secondary metabolism: food quality and production of aromas, flavors and toxins. Origin of agriculture and plant domestication. Characteristics of the main crops: Poaceae, Fabaceae, Rosaceae, Solanaceae, Brassicaceae. Domestication of Triticum turgidum var. dicoccum, Triticum turgidum var. durum e Triticum. Aestivum. Plant genetic improvement and “the green revolution”. Plant biotechnology for the development of crop plants resistant to environmental stress and/or herbicides and with high nutritional value. Primary and secondary metabolism. Secondary metabolism: terpenes, phenols, alkaloids, cyanogenic glycosides, glucosinolates, non-protein amino acids. Nutritional properties of secondary metabolites: metabolites with toxic effects and metabolites with positive effects on human health. Aromatic plants, spice plants and nervine plants (tea, coffee, cocoa). Natural dyes in food. Bioactive metabolites as food supplements. Primary metabolism: nutritional values of the different plant organs and nutrients bioavailability. Nutrients from plants: carbohydrates (sucrose and starch from different sources), soluble and insoluble dietary fibers, lipids and oils (olive oil and seed oil), proteins, minerals, vitamins and antioxidants. Effects of storage conditions and pre- and post-harvest diseases on nutritional quality of plant foods. Allergy and intolerances caused by plant substances present in crop species.
( reference books)
1. Piante alimentari. Biologia, composizione chimica, utilizzazione. Rinallo C.; Piccin. 2. Biologia delle Piante. Vol 2: Interazioni con l’ambiente e Domesticazione. Smith A. M. et al.; Zanichelli. 3. Lecture notes and scientific articles provided by the teacher
The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it
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6
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BIO/04
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40
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10
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Core compulsory activities
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ITA |
20410214 -
Nutrigenomica
(objectives)
The course will deal with diet-genome interactions and the application of nutritional strategies in health maintenance. Students should become familiar with the concept that external molecules present in our foods can affect human metabolism and gene expression in different cells and tissues. The course will address the utilization of the concept of “system biology” to nutrition
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6
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BIO/11
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48
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-
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Core compulsory activities
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ITA |
20402377 -
GENERAL PATHOLOGY
(objectives)
The aims of the course are: •to know the aetiology and the molecular mechanisms of the human diseases; •to know how to interpret the pathophysiological mechanisms of the human diseases
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6
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MED/04
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48
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Core compulsory activities
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ITA |
20410491 -
Fisiologia della risposta cellulare allo stress
(objectives)
The primary objective of the course is to enlarge and update student’s knowledge about the physiology and regulation of cellular functions mainly regarding the molecular mechanisms activated by cells in response to stressing conditions to maintain intracellular homeostasis.
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Derived from
20410491 Fisiologia della risposta cellulare allo stress in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 FIOCCHETTI MARCO
( syllabus)
Introduction to the molecular mechanisms of cellular functions regulation: membrane and nuclear receptors, mechanisms of intracellular signal transduction and amplification through protein’s post-translational modifications (e.g. phosphorylation). Definition of cellular stress and physiological tolerance range. Description of intracellular mechanisms, at DNA, RNA and protein levels, through which cells sense and respond to a broad variety of stressing conditions, focusing the attention mainly on physiological response and cell adaptation to extracellular stressing stimuli, including heat, osmotic, hypoxic, oxidative and nutrient stresses. Evaluation of intracellular organelles- specific stress response, including mitochondria- and ER-stress response. Description of down-stream molecular mechanisms activated by cells in response to stress and devote to re-establish the intracellular homeostasis and to adapt themselves to the changed conditions. Brief description of the abnormal stress response and adaptation in pathological cell degeneration (e.g. cancer). Definition and update of the state-of-art about the laboratory research approaches applied to the study of the physiological stress response and adaptation and the molecular mechanisms behind these functions.
( reference books)
All the study/research materials and PDF files of lessons are available at the Course's Moodle Web site: https://scienze.el.uniroma3.it/course/view.php?id=20
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6
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BIO/09
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48
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-
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-
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Core compulsory activities
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ITA |
20410495 -
Microbiomica
(objectives)
The goal of the Microbiomics course is to provide information about the structure, dynamics, evolution, and methods to investigate complex microbial communities as a whole. The rapid progress of omic sciences (genomics, transcriptomics, proteomics and metabolomics) combined with high-throughput genome sequencing, global gene expression analysis tools, and powerful analytical method of metabolites has made feasible the analysis of complex and diverse microbial communities and their products as a whole. The course is intended to provide state-of-art information on the impact of the microbiota in agriculture, food production, as well as animal and human health
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Derived from
20410495 Microbiomica in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 VISCA PAOLO
( syllabus)
BASIC TOPICS: Introduction to the genomics of prokaryotes and its study techniques (sequencing, annotation, gene family content, duplications and deletions); Horizontal gene transfer and mobilome; metagenomics; Transcriptomics and gene chips; Proteome and interactome; metabolomics; Genomics of single cells; Systems biology and health; Genetic manipulations - Expression of heterologous genes in bacteria - Evolution of bacterial genomes; Molecular phylogeny; Microbial taxonomy methods and species concept; Classification of prokaryotes; Culture analysis of microbial communities; Enrichment cultures; Isolation of single cells; Laser forceps; Flow cytometry; Microfluidic techniques; High-throughput systems; Staining methods; Fluorescent in-situ hybridization (FISH); Gene amplification methods for the analysis of microbial communities; Microarray analysis of the genetic and functional diversity of microorganisms; Environmental genomics and study methods; Measurement of microbial activity in nature; Examples of symbiosis: lichens, intestinal polymicrobial systems (rumen); General information on the huiman microbiome; Gastrointestinal microbiota; Oral and airway microbiota; Microbiota of the urogenital tract and skin; Development and evolution of the human microbiota; Studies on humans and in animal models; Colonization, microbial sequences and stability of the intestinal microbiota; Diseases associated with intestinal dysbiosis; Diseases associated with dysbiosis of the oral cavity, skin and genital tract; Antibiotics, resistome and induced alterations of the microbiome; Probiotics and prebiotics; Microbiota and intestine-brain axis. APPLICATIONS: Technologies for sequencing and NGS; Culturome and chemotaxonomy; Sequencing data preparation and analysis; Phylogeny based on 16S rRNA, 25-28S rRNA, ITS etc .; Tools to analyze the structure and dynamics of the microbiota: Whole Genome Sequencing (WGS) and metagenomics shotgun; Analytical databases, tools and pipelines (annotations); Resistome; Virulome; Virome. IN-DEPTH: illustration of monothematic articles on the microbiota of humans, animals and plants, in relation to physio-pathological alterations. Groundbreaking articles and reviews will be selected from scientific journals year by year.
( reference books)
"Brock biology of microorganisms" (English) by Michael T. Madigan (Author), Kelly S. Bender (Author), Daniel H. Buckley (Author), W. Matthew Sattley (Author), David A. Stahl (Author): chapters 9.1-9.14; 12.1-12.5; 12.9; 12.11-12.13; 13.5-13.10; 19.1-19.12; 23.1; 23.12, 23.13; 24.1-24.11 Microbiota in health and disease: from pregnancy to childhood. Editors Pamela D. Browne, Eric Claassen and Michael D. Cabana Published: 2017 Pages: 344. eISBN: 978-90-8686-839-1 | ISBN: 978-90-8686-294-8 https://doi.org/10.3920/978-90-8686-839-1 The human microbiota: how microbial communities affect health and disease / edited by David N. Fredricks. ISBN 978-0-470-47989-6
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6
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BIO/19
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48
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-
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-
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-
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Core compulsory activities
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ITA |
20410321 -
VIROLOGIA ANIMALE GENERALE ED APPLICATA
(objectives)
Deepening the knowledge about animal viruses (origins, classification, replicative strategies, virulence, immunoevasion mechanisms) and their applications (antiviral vaccines, viral vectors for gene expression, oncolytic viruses).
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Derived from
20410321 VIROLOGIA ANIMALE GENERALE ED APPLICATA in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 AFFABRIS ELISABETTA
( syllabus)
The course describes and compares the replication cycles of different animal viruses that infect vertebrate hosts to gain insight into: origin of viruses, structure, evolution, virus-cell interactions, host defense mechanisms and strategies of viral immunoevasion, methods of detection, identification and titration, antiviral drugs and profilaxis of viral infections. In particular, it will be described viruses of the following viral families: Parvoviridae, Poliomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae, Picornaviridae, Flaviviridae, Coronaviridae, Ortomixoviridae, Rabdoviridae, Retroviridae, Hepadnaviridae, . In addition, introduction to applied virology will be provided (i.e., vaccine development, viral vectors and oncolytic viruses). Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
All the detailed list of topics covered during the lessons, the PDF files of power point presentations used during the course and further materials are available in the Moodle platform of the course.
Adopted text book: - N. J. Dimmock, A. J. Easton, K. N. Leppard - Introduction to Modern Virology - Seventh edition 2016 -Wiley Blackwell - ISBN 978-1-119-97810-7 - Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press (this text book is more detailed) to complement the PDF files of the power point used by the teacher during the course. Mandatory integrations of the PDF files regarding viral vectors (adenoviral, adeno-associated, retroviral and lentiviral vectors, poxvirus-based vectors) and oncolitic viruses (general introduction + oncolytic adenoviruses) can be found herebelow: - Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (on line access through Roma Tre Scientific Library) - Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159. - Carina Elsner and Jens Bohne - The retroviral vector family: something for everyone. – Virus Genes 2017, 53:714-722 The teacher receives by appointment via email (elisabetta.affabris@uniroma3.it) or at the end of the lessons.
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6
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BIO/19
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40
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-
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10
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-
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Core compulsory activities
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ITA |
20410527 -
Neurobiologia molecolare dello sviluppo
(objectives)
The Course aims to provide the student with advanced knowledge and skills in molecular biology of neurodevelopment, with particular reference to the central nervous system of vertebrates.
The aim of the first module is to provide the student with general knowledge about the cellular processes that lead, in the course of early and late embryogenesis, to the formation of the central nervous system from the neural tube. Advanced concepts on morphogenetic processes ensuring the achievement of the general cytoarchitecture of telecephalon, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord will be provided. In particular, the student will acquire knowledge and skills on neuronal migration processes (under normal and pathological conditions) that lead to the layering of the cerebral cortex, spinal cord, and cerebellum. Advanced knowledge and expertise will also be provided on the cellular processes underlying adult neurogenesis and neurodegeneration, focusing on the role of apoptosis and autophagy. The student, through an interactive teaching will acquire skills in the study of pathological conditions, such as the most common neurodegenerative disorders (Alzheimer's disease, Parkinson's disease, Huntington's chorea).
The aim of the second module is to provide the student with general knowledge regarding the gene expression underlying the differentiation and morphogenesis of the nervous tissue. These molecular mechanisms will be addressed in different study models. It will be highlighted how neurogenesis and plasticity have profound functional implications in the nervous system throughout the life of the individual. The student, by means an interactive teaching, will acquire skills on new biomolecular technologies to study the development and functionality of the nervous system under physiological and pathological conditions.
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Derived from
20410527 Neurobiologia molecolare dello sviluppo in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 MORENO SANDRA, CERVELLI MANUELA, Nazio Francesca
( syllabus)
Overview of the development of multicellular organisms. Ectodermal derivation of neural tissue in invertebrates and vertebrates. Primary neurulation: cellular and molecular mechanisms of neural plate formation, neural folds, neural groove and neural tube. Secondary neurulation. Neural tube closure defects in mammals: genetic and environmental causes. Morphogenesis and differentiation of the brain and spinal cord. Role of cell death in central nervous system morphogenesis: apoptotic mechanisms. Layering of cerebral and cerebellar cortical structures. Congenital diseases related to neuronal migration defects: the case of peroxisomal disorders. Neural stem cells: neuronal and glial differentiation. Neurogenesis in the adult brain: the subventricular and subgranular areas of the mammalian dentate gyrus. Pathways regulating activity of the neurogenic niche. Neurogenesis and neurodegeneration in the senescent brain. Neurodegenerative diseases related to aging: common features and anatomical and genetic-molecular specificities. Protein aggregates, role of apoptosis and autophagy and therapies against neurodegeneration, based on stem cell transplants and endogenous regeneration.
( reference books)
Gilbert: "Developmental Biology", 9th Edition, Sinauer Associates
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3
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BIO/06
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20
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-
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5
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-
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Core compulsory activities
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3
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BIO/11
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20
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5
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Core compulsory activities
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ITA |
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Optional group:
AFFINI E INTEGR - (show)
![Info](/images/iconai.png) |
12
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20410207 -
Biochimica della nutrizione
(objectives)
The Nutrition Biochemistry course is aimed at providing to the students the basic knowledge to understand the molecular mechanisms by which the human organism uses macro- and micro-nutrients to produce the energy needed to maintain the order which characterizes every living being. In particular, the objectives of the course are the comprehension of the pathways through which the different groups of nutrients (carbohydrates, lipids, proteins, vitamins and minerals) and other substances of natural and non-natural origin (ethanol, stimulant beverages, xenobiotics) are assimilated and transformed in the human body, and how they influence its metabolic status. Finally, one of the aims is also that of illustrating the production of reactive oxygen species linked to metabolism and the protective role exerted by antioxidant compounds of dietary origin
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POLTICELLI FABIO
( syllabus)
- Metabolism of the main alcoholic and psychoactive drinks of nutritional value - Signal transduction and regulation of metabolic pathways - Metabolic regulation of the nutrition-fasting cycle - Reactive oxygen species - Endogenous and exogenous antioxidants - Xenobiotics
( reference books)
- Leuzzi, Bellocco, Barreca “Biochimica della Nutrizione” Ed. Zanichelli
- Cozzani, Dainese "Biochimica degli alimenti e della nutrizione" Ed. Piccin.
- General Biochemistry textbooks adopted for the Biochemistry Course of the bachelor degree in Biological Sciences.
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6
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BIO/10
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48
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-
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-
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-
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Related or supplementary learning activities
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ITA |
20410212 -
Genetica umana applicata
(objectives)
The main aims of the course are: 1. To consolidate knowledge in Human Genetics acquired during previous basic courses, with particular reference to biological mechanisms involved in genetic diseases 2. To carry on theorical notions on a practical point of view by the knoledge of diagnostic laboratory methods and strategies
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6
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BIO/18
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48
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-
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-
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-
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Related or supplementary learning activities
|
ITA |
20410314 -
GENETICA DEI SISTEMI COMPLESSI
(objectives)
The course aim to provide a wider and deeper outlook of genetics topics not addressed in the course of genetics. Special attention will be paied to the role of human genome (and epigenome) in the regulation of complex biological processes and in the individual response to endogenous and exogenous factors.
-
BERARDINELLI FRANCESCO
( syllabus)
- GENOME (ORGANIZATION, STRUCTURAL GENOMICS, COMPARATIVE GENOMICS AND FUNCTUNIAL GENOMICS). EPIGENOME (METHYLATION, HISTONE CODE, CHROMATIN 3D ARCHITECTURE, NON-CODING RNA). EXAMPLES OF EPIGENETIC REGULATION: CELL DIFFERENTIATION AND GENETIC MECHANISMS OF SEX DETERMINATION (HOW DOES SEX IS GENETICALLY DETERMINED, INACTIVATION OF X CHROMOSOME, SEX-DEPENDENT PHONOTYPIC EFFECTS). - CELL CYCLE REGULATION, SENESCENCE AND APOPTOSIS. GENETIC OF AGING (GENETIC THEORIES OF AGING, GERONTOGENES AND GENES INVOLVED IN LONGEVITY, AGING IN ANIMAL MODELS, MOLECULAR AND CELLULAR AGING). CANCER GENETICS (ONCOGENES, ONCO-SUPPRESSOR GENES, CHROMOSOMAL REARRANGEMENTS INVOLVED IN CANCER, THE CANCER STEM CELLS THEORY). INDIVIDUAL GENETIC RESPONSE AND PHARMACOGENETICS.
( reference books)
- SCIENTIFIC ARTICLES - TOM STRACHAN and ANDREW READ, HUMAN MOLECULAR GENETICS, 5th Edition, CRC PRESS (Taylor & Francis Group)
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6
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BIO/18
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40
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-
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10
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-
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Related or supplementary learning activities
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ITA |
20401653 -
BIOCHEMISTRY AND MOLECULAR CLINICAL BIOLOGY
(objectives)
Main goals of the course are: i) to provide an overview of the activities of a clinical laboratory looking at the organizational context and current business; ii) to illustrate the various stages which are at the basis of main analytical methods, either molecular or biochemical, used in biomedical laboratories for modern diagnostic purposes; iii) to provide the key of interpretation for the obtained results from a pathophysiological point of view. To promote a critical approach towards laboratory diagnosis.
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BARONI SILVIA
( syllabus)
LABORATORY MEDICINE AND CLINICAL BIOCHEMISTRY BIOCHEMICAL TESTS AND LABORATORY MANAGEMENT PREANALYTICAL, ANALYTICAL AND BIOLOGICAL VARIABILITY REFERENCE VALUES • QUALITY CONTROL ASSURANCE • ANALYTICAL METHODS IMMUNOCHEMISTRY AND INTERFERENCES • LABORATORY ANDCARDIOVASCULAR DISEASES LIPOPROTEIN ALTERATIONS LABORATORY AND DIABETES MELLITUS MARKERS OF CARDIAC DISEASES • LABORATORY AND RENAL FUNCTION URINALYSIS • LABORATORY AND LIVER • LABORATORY AND ONCOLOGY • LABORATORY AND MALE INFERTILITY • INTRODUCING CLINICAL MOLECULAR BIOLOGY
( reference books)
Clinical Biochemistry 6th Edition- Elsevier 2018 An Illustrated Colour Text Michael Murphy Rajeev Srivastava Kevin Deans
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6
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BIO/12
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48
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-
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-
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Related or supplementary learning activities
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ITA |
20401676 -
FRESH WATER ECOLOGY
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I modulo
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3
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BIO/07
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20
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5
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-
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-
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Related or supplementary learning activities
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ITA |
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II modulo
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3
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BIO/07
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20
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5
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-
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Related or supplementary learning activities
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ITA |
20401664 -
MICROBIAL GENETICS
(objectives)
The main educational goal of the genetics of microorganisms course is to provide students with knowledge on: 1) the prokaryotic genome and the mechanisms that ensure its replication, transcription, translation and integrity throughout generations; 2) the regulation mechanisms of the genetic expression in bacteria; 3) the mechanisms responsible for horizontal gene transfer (conjugation, transformation and transduction); 4) the molecular basis of cellular differentiation and adaptive response. Students will also acquire basic knowledge on genetic engineering and omic approaches to study microbial communities.
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IMPERI FRANCESCO
( syllabus)
The course aims at providing to the student: - adequate knowledge and understanding of advanced methodologies in bacterial genetics, appropriate methodologies and communication skills for the study of genotype-phenotype relationships in bacteria; - adequate applied knowledge for the evaluation and drafting of experimental approaches, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgments).
Program - structure, organization, functioning and evolution of the bacterial chromosome; - methods for the sequencing and analysis of bacterial genomes; - structure, organization, function and evolution of mobile genetic elements (plasmids and transposons); - genetic and molecular bases of horizontal gene transfer; - genetic and molecular bases of gene expression regulation and bistability in bacteria; - genetic bases and experimental approaches for the study of antibiotic resistance and tolerance; - methods for the identification and validation of essential genes; - approaches for the identification and study of unculturable microorganisms
( reference books)
Selected topics will be available on suggested Bacterial Genetics books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices. Innovative teaching will be promoted by sharing power-point presentations illustrating the main topics of the course via email. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.
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6
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BIO/18
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40
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10
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-
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20410278 -
MICROBIOLOGIA AMBIENTALE
(objectives)
The main aims of the course are: 1. acquire knowledge of the fundamental role of microorganisms in ecosystems and of the factors affecting their distribution and interactions with other organisms: - metabolic and functional biodiversity, structure and dynamics of microbial communities - bacteria and archea taxonomic groups 2. knowledge of traditional, molecular and cultivation-independent methods for identification/typing and analysis of microbial populations 3. evaluation of the multiple potential applications of environmental microorganisms also as bioindicators 4. acquisition of critical skills by reading scientific articles.
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6
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BIO/19
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44
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5
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ITA |
20410486 -
Laboratorio di neurobiologia cellulare
(objectives)
The aims of this course are: 1)to gain knowledge of the specialized cellular architecture of glia and neurons; 2)to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
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PERSICHINI TIZIANA
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology. Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity. Topics: The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA” – THIRD EDITION – 2012 OXFORD UNIVERSITY PRESS
Slides in PDF format of all the lectures will be provided by the teacher.
The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it
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6
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BIO/06
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32
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20
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20410491 -
Fisiologia della risposta cellulare allo stress
(objectives)
The primary objective of the course is to enlarge and update student’s knowledge about the physiology and regulation of cellular functions mainly regarding the molecular mechanisms activated by cells in response to stressing conditions to maintain intracellular homeostasis.
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FIOCCHETTI MARCO
( syllabus)
Introduction to the molecular mechanisms of cellular functions regulation: membrane and nuclear receptors, mechanisms of intracellular signal transduction and amplification through protein’s post-translational modifications (e.g. phosphorylation). Definition of cellular stress and physiological tolerance range. Description of intracellular mechanisms, at DNA, RNA and protein levels, through which cells sense and respond to a broad variety of stressing conditions, focusing the attention mainly on physiological response and cell adaptation to extracellular stressing stimuli, including heat, osmotic, hypoxic, oxidative and nutrient stresses. Evaluation of intracellular organelles- specific stress response, including mitochondria- and ER-stress response. Description of down-stream molecular mechanisms activated by cells in response to stress and devote to re-establish the intracellular homeostasis and to adapt themselves to the changed conditions. Brief description of the abnormal stress response and adaptation in pathological cell degeneration (e.g. cancer). Definition and update of the state-of-art about the laboratory research approaches applied to the study of the physiological stress response and adaptation and the molecular mechanisms behind these functions.
( reference books)
All the study/research materials and PDF files of lessons are available at the Course's Moodle Web site: https://scienze.el.uniroma3.it/course/view.php?id=20
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6
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BIO/09
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48
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20410493 -
Psicobiologia
(objectives)
The course will address the main topics of interest in psychobiology, with the aim of promoting a basic knowledge of the subject, of the main experimental paradigms and methods used in psychobiology. The aim of the course is therefore to provide knowledge of the biological and neuropsychological fundamentals and correlates of behavior and psychological functions in humans and animals, as well as the main methods and techniques used in psychobiological research.
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TREZZA VIVIANA
( syllabus)
Introduction to psychobiology: field of investigation and history Introduction to the biological basis of behavior Genes per environment interaction in typical and atypical brain development Cognitive, affective and social development in childhood and adolescence Learning and conditioning: neural substrates, pathological aspects, experimental paradigms Memory: types of memory, neural substrates, pathological aspects, experimental paradigms Emotion and motivation: neural substrates, pathological aspects, experimental paradigms Psychotropic substances and the neurobiology of reward processes Social behavior: neural substrates, pathological aspects, experimental paradigms
( reference books)
- The slides of the lessons will be made available to students
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6
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BIO/14
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48
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ITA |
20410496 -
Laboratorio di neuroscienze
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20401671 -
GENERAL PATHOLOGY
(objectives)
The aims of the course are: 1) to know the aetiology and the molecular mechanisms of the human diseases; 2)to know how to interpret the pathophysiological mechanisms of the human diseases.
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6
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MED/04
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48
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ITA |
20410495 -
Microbiomica
(objectives)
The goal of the Microbiomics course is to provide information about the structure, dynamics, evolution, and methods to investigate complex microbial communities as a whole. The rapid progress of omic sciences (genomics, transcriptomics, proteomics and metabolomics) combined with high-throughput genome sequencing, global gene expression analysis tools, and powerful analytical method of metabolites has made feasible the analysis of complex and diverse microbial communities and their products as a whole. The course is intended to provide state-of-art information on the impact of the microbiota in agriculture, food production, as well as animal and human health
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VISCA PAOLO
( syllabus)
BASIC TOPICS: Introduction to the genomics of prokaryotes and its study techniques (sequencing, annotation, gene family content, duplications and deletions); Horizontal gene transfer and mobilome; metagenomics; Transcriptomics and gene chips; Proteome and interactome; metabolomics; Genomics of single cells; Systems biology and health; Genetic manipulations - Expression of heterologous genes in bacteria - Evolution of bacterial genomes; Molecular phylogeny; Microbial taxonomy methods and species concept; Classification of prokaryotes; Culture analysis of microbial communities; Enrichment cultures; Isolation of single cells; Laser forceps; Flow cytometry; Microfluidic techniques; High-throughput systems; Staining methods; Fluorescent in-situ hybridization (FISH); Gene amplification methods for the analysis of microbial communities; Microarray analysis of the genetic and functional diversity of microorganisms; Environmental genomics and study methods; Measurement of microbial activity in nature; Examples of symbiosis: lichens, intestinal polymicrobial systems (rumen); General information on the huiman microbiome; Gastrointestinal microbiota; Oral and airway microbiota; Microbiota of the urogenital tract and skin; Development and evolution of the human microbiota; Studies on humans and in animal models; Colonization, microbial sequences and stability of the intestinal microbiota; Diseases associated with intestinal dysbiosis; Diseases associated with dysbiosis of the oral cavity, skin and genital tract; Antibiotics, resistome and induced alterations of the microbiome; Probiotics and prebiotics; Microbiota and intestine-brain axis. APPLICATIONS: Technologies for sequencing and NGS; Culturome and chemotaxonomy; Sequencing data preparation and analysis; Phylogeny based on 16S rRNA, 25-28S rRNA, ITS etc .; Tools to analyze the structure and dynamics of the microbiota: Whole Genome Sequencing (WGS) and metagenomics shotgun; Analytical databases, tools and pipelines (annotations); Resistome; Virulome; Virome. IN-DEPTH: illustration of monothematic articles on the microbiota of humans, animals and plants, in relation to physio-pathological alterations. Groundbreaking articles and reviews will be selected from scientific journals year by year.
( reference books)
"Brock biology of microorganisms" (English) by Michael T. Madigan (Author), Kelly S. Bender (Author), Daniel H. Buckley (Author), W. Matthew Sattley (Author), David A. Stahl (Author): chapters 9.1-9.14; 12.1-12.5; 12.9; 12.11-12.13; 13.5-13.10; 19.1-19.12; 23.1; 23.12, 23.13; 24.1-24.11 Microbiota in health and disease: from pregnancy to childhood. Editors Pamela D. Browne, Eric Claassen and Michael D. Cabana Published: 2017 Pages: 344. eISBN: 978-90-8686-839-1 | ISBN: 978-90-8686-294-8 https://doi.org/10.3920/978-90-8686-839-1 The human microbiota: how microbial communities affect health and disease / edited by David N. Fredricks. ISBN 978-0-470-47989-6
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6
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BIO/19
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48
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20401777 -
PUBLIC HEALTH
(objectives)
General objectives: at the end of the course, the student will be able to design epidemiological studies to address public health problems and to identify activities to prevent chronic and infectious diseases.
Specific objectives: • know the major mortality and morbidity indicators • to identify the epidemiological study more appropriate to describe the burden of major diseases in the population and to assess the relation between diseases and possible risk associated to them •to interpret epidemiological data •to discuss the importance of the health education and of the health promotion •to list and discuss problems related to water supply, sanitation and waste management •to argue about necessary controls/monitoring to protect the environment and the community •to design health promotion programmes.
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6
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MED/42
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48
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20410259 -
ENTOMOLOGIA
(objectives)
The main formative targets are: 1) the acquisition of a basic knowledge on morphology, anatomy, physiology and evolution of the main insect groups; 2) the acquisition of both theoretical and practical knowledge on systematics and classification of the hexapoda; 3) the construction of a solid cultural background for an advanced discussion on causes and effects of the amazing evolutionary success and diversity of insects and on their importance in basic and applied studies; 4) the acquisition of the tools for the insect taxonomic identification, expecially for those orders and families present in the italian fauna; 5) to stimulate the curiosity and the ability of critically observe the nature; 6) the self-evaluation by the student of the competence whitin the animal biology, and in particular in the entomology.
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Derived from
20410259 ENTOMOLOGIA in Biodiversità e gestione degli ecosistemi LM-6 DI GIULIO ANDREA
( syllabus)
1) Introduction: aim of the course; entomology in the history of Zoology and more generally in the history of science; role of entomology in modern biological disciplines; basic and applied entomology; relationships of insects with man and the environment. 2) Generalities on Arthropods; phylogenesis problems of Arthropods; insects in the evolution of terrestrial Arthropods; general information on the Atelocerata, and in particular on the Hexapods; outline of paleontology of insects; insect origin; significance of the evolutionary success of insects. 3) Insects as land animals; exoskeleton; structure of the integument and cuticle; chemical and physical colors. External anatomy: regions of the body; sclerites; primary and secondary segmentation; appendices and wings. 4) Movement: musculature and endoskeleton; the race; the jump; origin and mechanics of flight; adaptations to aquatic life and importance of surface forces, swimming; the excavation; raptorial and phoretic limbs. 5) Breathing; breathing in the aquatic environment; water reabsorption; excretion; adaptations to temperature changes. 6) Nutrition: cephalic appendages and mouthparts; anatomy and physiology of the digestive system; food strategies. 7) Circulatory system. 8) Reproduction: structure and functional morphology of the segments and genital appendages; reproductive systems and reproductive behaviors; parthenogenesis; pedogenesis; polyembryony; meaning of heterogony; eggs; sperm. 9) Embryonic and postembryonic development: growth and metamorphosis; types of larvae and types of metamorphosis; diapause; physiology; endocrine glands. 10) Communication in insects. Nervous system: components and functioning. Endocrine system: hormones and endocrine organs. Exocrine glands. Perception of the environment. Compound eyes. Vision. Ocelli. Mechanoreceptors. Coloration. Light production. Mechanical communication: production of sound and vibrations of the substrate. Chemical communication: pheromones and secretions of interspecific significance. 11) Intraspecific relationships; parental care; society; interspecific relationships; predation; parasitism. 12) Orientation; migrations; dispersion. 13) Classification: diagnostic characters at the level of orders and outline of the most important families, with particular regard to the Italian fauna and the aspects of biology and ecology. Phylogeny of insects. Esapodi Apterigoti: COLLEMBOLA, PROTURA, DIPLURA, MICROCORYPHIA, ZYGENTOMA. Paleotteri: EPHEMEROPTERA, ODONATA. Polineotteri (orthopteroid orders): PLECOPTERA, EMBIOPTERA, DICTYOPTERA, ISOPTERA, GRYLLOBLATTODEA, DERMAPTERA, PHASMIDA, ORTOPTERA, ZORAPTERA, MANTOFASMATODEA. Paraneoptera (emitteroid orders): PSOCOPTERA, PHTHIRAPTERA, THYSANOPTERA, HETEROPTERA. Homoptera. Oligoneotteri: MECOPTERA, TRICHOPTERA, LEPIDOPTERA, DIPTERA, SIPHONAPTERA, MEGALOPTERA, RAPHIDIOPTERA, PLANIPENNIA, COLEOPTERA, STREPSIPTERA, HYMENOPTERA. 14) Aspects of applied entomology: medical entomology, agricultural entomology, entomology in environmental assessment, insect conservation, urban entomology, veterinary entomology, entomology applied to conservation of cultural heritage.
( reference books)
P.J. Gullan & P.S. Cranston “Lineamenti di Entomologia” Zanichelli R.G. Davies “Lineamenti di Entomologia” Zanichelli
M. Chinery “Guida agli insetti d’Europa” Muzzio
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6
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BIO/05
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32
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20
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ITA |
20401821 -
IMMUNOLOGY
(objectives)
Cellular and molecular mechanisms involved in the immune response with a particular interest for those implicated in the response against pathogens.
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6
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MED/04
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48
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20410321 -
VIROLOGIA ANIMALE GENERALE ED APPLICATA
(objectives)
Deepening the knowledge about animal viruses (origins, classification, replicative strategies, virulence, immunoevasion mechanisms) and their applications (antiviral vaccines, viral vectors for gene expression, oncolytic viruses).
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AFFABRIS ELISABETTA
( syllabus)
The course describes and compares the replication cycles of different animal viruses that infect vertebrate hosts to gain insight into: origin of viruses, structure, evolution, virus-cell interactions, host defense mechanisms and strategies of viral immunoevasion, methods of detection, identification and titration, antiviral drugs and profilaxis of viral infections. In particular, it will be described viruses of the following viral families: Parvoviridae, Poliomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae, Picornaviridae, Flaviviridae, Coronaviridae, Ortomixoviridae, Rabdoviridae, Retroviridae, Hepadnaviridae, . In addition, introduction to applied virology will be provided (i.e., vaccine development, viral vectors and oncolytic viruses). Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
All the detailed list of topics covered during the lessons, the PDF files of power point presentations used during the course and further materials are available in the Moodle platform of the course.
Adopted text book: - N. J. Dimmock, A. J. Easton, K. N. Leppard - Introduction to Modern Virology - Seventh edition 2016 -Wiley Blackwell - ISBN 978-1-119-97810-7 - Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press (this text book is more detailed) to complement the PDF files of the power point used by the teacher during the course. Mandatory integrations of the PDF files regarding viral vectors (adenoviral, adeno-associated, retroviral and lentiviral vectors, poxvirus-based vectors) and oncolitic viruses (general introduction + oncolytic adenoviruses) can be found herebelow: - Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (on line access through Roma Tre Scientific Library) - Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159. - Carina Elsner and Jens Bohne - The retroviral vector family: something for everyone. – Virus Genes 2017, 53:714-722 The teacher receives by appointment via email (elisabetta.affabris@uniroma3.it) or at the end of the lessons.
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6
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BIO/19
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40
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10
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ITA |
20402508 -
NATURAL PRODUCTS AND ENVIRONMENTAL CHEMISTRY
(objectives)
The course aims at giving: 1) a general knowledge of the biosynthetic pathways of secondary metabolism; 2) information about the main natural products derived from microorganism, plants and animals and their role in natural environment; 3) knowledge on the structure/biological activity relationship of the main secondary metabolites used in pharmaceutical and healthcare industry. 4) attainment of the basic educational background necessary to understand the chemical equilibria that regulate the origins, the transport, the transformations and the effects of the environmental chemical species, either of natural or human origin.
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Derived from
20402508 CHIMICA DELLE SOSTANZE NATURALI E DELL'AMBIENTE in Biodiversità e gestione degli ecosistemi LM-6 N0 TOFANI DANIELA
( syllabus)
-Introductory notes on the chemistry of natural substances. Characteristics of secondary metabolites. Allelopatic substances. The biosintetic ways of secondary metabolism. Metabolism of acetate. Prostaglandins. Aromatic polychetides. Anthraquinones. Cannabinoids. Aflatoxins. Tetracycline. The metabolism of shikimato. Biogenesis of shikimato. Cinnamic acids. Lignins. Fenilpropani. Benzoic acids. Coumarins. Flavonoids. Tannins. Isoflavones. Terpenoid chinons. The metabolism of the mevalonate. Biogenesis of mevalonic acid. The various types of terpens and derived compounds. Iridoids. Gibberellins. Structural characteristics of animal steroids. Phytosterols. Alcaloids: definition, original amino acids and nomenclature. Biosynthesis and activities of the main types of alcaloids: cocaine, nicotine, curari, morphine, loganin, lysergic acid, coniine. Notes on carbohydrates. Cianogenic glucosides. Streptomycin. Secondary metabolites of peptidic origin. Interferons. Opious peptides. Peptidic toxins: ricina and botulino. Penicillins. Basic principles of environmental chemistry. Interactions between the various spheres in which the terrestrial environment is divided. The atmosphere: notes on training and depositing of the ozone (buco dell'ozono); effect of cfc and halon. Notes on oxidizing and polluting species and their monitoring. The particulate: ipa and phthalates. The serra effect: main gas-greenhouse and their gwp. The kyoto protocol. The hydrosphere. Chemical-physical properties of water and water bodies. TIC, TOC, TC. Biodegradable organic pollutants (COD, BOD5), of difficult oxidation and toxic (LD50 and LOD50). Bioaccumulation and biomagnification. Metabolism of some pesticides.
( reference books)
P.M. DEWICK : "Medicinal Natural Products" Wiley Ed. C. BAIRD, M. CANN “Environmantal Chemistry”, Slides of the pwp presentation of each lesson.
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6
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CHIM/06
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48
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20410301 -
ECOLOGIA E GESTIONE DEGLI ECOSISTEMI COSTIERI
(objectives)
Major goals of this course are: to acquire an appropriate knowledge of the biodiversity and of general principles of the ecology of coastal ecosystems and their relationship with other ecological branches (plant and animal ecology). Other goal of this course is the growth of a thorough interest to ecological problems discussing the relationship between man and nature focusing in the coastal environment, analyzing main disturbance factors. The course is also intended to develop basic insights for the acquisition (sampling and monitoring), organization and interpretation of ecological data recorded in coastal ecosystems.
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Derived from
20410301 ECOLOGIA E GESTIONE DEGLI ECOSISTEMI COSTIERI in Biodiversità e gestione degli ecosistemi LM-6 ACOSTA ALICIA TERESA ROSARIO, DI GIULIO ANDREA
( syllabus)
Ecology and plant diversity of coastal ecosystems
Coastal ecosystems, the transition between land and sea. Environmental gradients. Ecotones and transitions. Plant diversity in coastal, rocky and lagoon coastal environments. Major features and distribution throughout the world. Plant communites in Italy and Europe (Mediterranean and Atlantic). Analyses of main plant communities and habitats (European Directive 92/43/EEC (Habitat Directive) present in Italy. Conservation problems. Sampling tecniques and data analyses.
Ecologia e diversità faunistica delle coste sabbiose, delle lagune costiere e delle coste rocciose The animal communities of coastal dunes and their ecological relations with plants. Interactions between the animal communities of coastal dunes and Mediterranean evergreen scrub. The fauna of coastal wetlands and their importance as sites of bird nesting and winter refuges. The Ramsar Convention: conservation and management issues in wetlands. Stranding and recovery of marine vertebrates (whales, dolphins and sea turtles). Nesting of sea turtles and their conservation. Coexistence of marine and land animals in the intertidal zone. Fauna of tide pools. Sea bird nesting on rock cliffs: conservation and management issues.
Conservation and management of coastal ecosystems
Conservation of coastal ecoystems. Disturbance factors. The role of disturbance on coastal ecosystems. Response to disturbance. Endangered species. Alien species. Bioindicators. Environmental quality and conservation status. Conservation and Management.
( reference books)
Acosta A. & Ercole S. 2017. Gli habitat delle coste sabbiose italiane: ecologia e problematiche di conservazione. Quaderni ISPRA (Istituto Superiore per la Protezione e la Ricerca Ambientale 215/2015.
Ruffo, S. (a cura di). (2002). Dune e spiagge sabbiose. Ambienti fra terra e mare. Quaderni Habitat, 4. Ministero dell’Ambiente e della Tutela del Territorio. Scaricabile dal sito del Ministero.
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4
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BIO/03
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16
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20
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Attività formative affini ed integrative
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2
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BIO/05
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16
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Attività formative affini ed integrative
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ITA |
20410213 -
Nutrienti di origine vegetale
(objectives)
The course "Nutrients of plant origin" is part of the Plant Physiology training activities of the Master of Science in Biology for Molecular, Cellular and Patho-physiological Research. This course provides basic knowledge relating to the qualitative and quantitative composition and nutritional properties of plant-based foods and their derived processed counterparts. In particular, the course provides 1) an overview of the plants used for human nutrition and their use for the preparation of food products, with an outline of the influence of processing processes on the nutritional properties; 2) an in-depth picture of the main nutrients, aromas and other substances useful for human nutrition present in food of plant origin; 3) a summary of the anti-nutritional factors, allergens and toxic compounds present in some foods of plant origin.
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CONA ALESSANDRA
( syllabus)
Climate change and food security. Plant varieties and phenotypic plasticity. Effect of environmental conditions on primary and secondary metabolism: food quality and production of aromas, flavors and toxins. Origin of agriculture and plant domestication. Characteristics of the main crops: Poaceae, Fabaceae, Rosaceae, Solanaceae, Brassicaceae. Domestication of Triticum turgidum var. dicoccum, Triticum turgidum var. durum e Triticum. Aestivum. Plant genetic improvement and “the green revolution”. Plant biotechnology for the development of crop plants resistant to environmental stress and/or herbicides and with high nutritional value. Primary and secondary metabolism. Secondary metabolism: terpenes, phenols, alkaloids, cyanogenic glycosides, glucosinolates, non-protein amino acids. Nutritional properties of secondary metabolites: metabolites with toxic effects and metabolites with positive effects on human health. Aromatic plants, spice plants and nervine plants (tea, coffee, cocoa). Natural dyes in food. Bioactive metabolites as food supplements. Primary metabolism: nutritional values of the different plant organs and nutrients bioavailability. Nutrients from plants: carbohydrates (sucrose and starch from different sources), soluble and insoluble dietary fibers, lipids and oils (olive oil and seed oil), proteins, minerals, vitamins and antioxidants. Effects of storage conditions and pre- and post-harvest diseases on nutritional quality of plant foods. Allergy and intolerances caused by plant substances present in crop species.
( reference books)
1. Piante alimentari. Biologia, composizione chimica, utilizzazione. Rinallo C.; Piccin. 2. Biologia delle Piante. Vol 2: Interazioni con l’ambiente e Domesticazione. Smith A. M. et al.; Zanichelli. 3. Lecture notes and scientific articles provided by the teacher
The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it
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6
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BIO/04
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40
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10
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20410214 -
Nutrigenomica
(objectives)
The course will deal with diet-genome interactions and the application of nutritional strategies in health maintenance. Students should become familiar with the concept that external molecules present in our foods can affect human metabolism and gene expression in different cells and tissues. The course will address the utilization of the concept of “system biology” to nutrition
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6
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BIO/11
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48
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ITA |
20410270 -
LABORATORIO DI BIOTECNOLOGIE DEI MICRORGANISMI
(objectives)
The main educational goals of the course are: 1) acquisition of knowledge on advanced methodologies in microbial biotechnology; 2) acquisition of appropriate methodologies and communication skills for the isolation, observation, description and engineering of industrial microorganisms; 3) acquisition of applied knowledge for drafting and executing experimental protocols; 4) acquisition of knowledge and skills for retrieving relevant scientific literature from on-line databases and for critically reviewing it. The expected learning outcomes are the increase of the student theoretical knowledge in the field of microbial biotechnology, of its practical skills for the design and execution of laboratory experiments, and of its critical ability to search and evaluate scientific literature data.
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RAMPIONI GIORDANO
( syllabus)
The course aims at providing to the student: - adequate knowledge and understanding on advanced methodologies in microbial biotechnology, appropriate methodologies and communication skills for the isolation, observation, description and engineering of industrial microorganisms; - adequate applied knowledge for drafting and executing experimental protocols, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).
Programme During the laboratory practices and the corresponding theoretic lessons, the following topics will be covered: - isolation of microorganisms of industrial relevance from the environment, preparation of growth media and cultivation techniques; - transposon–based mutagenesis (generation and screening of mutant libraries); - genetic transfer processes (conjugation and transformation); - use of whole-cell bacterial biosensors for gauging specific molecules produced by other microorganisms. The following topics will be covered in additional theoretic lessons: - development of innovative therapeutic strategies based on biotechnological processes; - development of biosensors and reporter systems for the identification of new drugs; - application of synthetic biology to microbial biotechnology; - properties of the regulatory networks and their application to biotechnological processes.
( reference books)
Selected topics will be available on suggested Microbiology and Microbial Biotechnology books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices. Innovative teaching will be promoted by cloud-sharing of power-point presentations illustrating the main topics of the course. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics. Students will be received at the best of their convenience if an appointment has been fixed at the end of the lessons or by email.
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6
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CHIM/11
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53
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20410487 -
Ecologia animale II
(objectives)
The course aims at providing the theoretical and practical bases of the ecology of animal communities and of macroecology, and to deepen some aspects of population ecology and the ecology of animal behaviour that require advanced knowledge that can be acquired with attending the course on Animal Ecology. - The student will learn the characteristics of animal communities, the descriptors to define the study methods to analyse their structure. The techniques to quantitatively analyse the relationships between the organisms belonging to the same community will be illustrated. Some models will be proposed to conduct community simulation analyses, the criteria for choosing the model and sampling techniques most suitable for the animal groups analysed. - The study of animal communities and macroecology will be organized in different modules. (i) Co-occurrence: the co-occurrence module allows to test non-random models of co-occurrence of species in a presence-absence matrix; (ii) Macroecology: the study of the partitioning of physical space and ecological resources by species. Macro-ecological studies consist of the analysis of traits at the species level, such as body size, geographical area and average abundance, measured at large spatial scales; (iii) Niche overlap: the study of niche overlap has a long history in community ecology. Simple theories of of limiting similarity y and ecological character displacement predict that interspecific competition will lead to a reduction in the niche overlap of competing species. Testing this idea with empirical data leads to three questions: 1) which aspects of the niche should be measured? 2) How can the niche overlap between species pairs be quantified? 3) how much niche overlap would you expect in the absence of competition? (iv) Overlap of body size: this module allows to test for unusual patterns in the body sizes of coexisting species, and to compare those patterns to what might be expected in a random assemblage that was not structured by interspecific interactions; (v) Diversity of communities: the diversity of species is a central object of study both in the basic and applied ecology of communities. Two main issues will be addressed in the study of species diversity. The first is how can we quantify the diversity of an assemblage, and second, how we can compare statistically the diversity of two different assemblages; (vi) Functional groups or guilds: groups of species within a community that share common resources are considered guilds. This module will allow to incorporate the guild structure into community analyses. - Through simulations in the laboratory and in the field, the student will learn the dynamics that determine the organization and structure of some selected animal communities. - Interactive computer programs will be used for the analysis of null models in community ecology to test community models with experimental and non-experimental data. The Monte Carlo randomizations will be illustrated as an analytical method to compare real communities with "pseudo-communities" created using different algorithms. The wide applicability of null models in applied and basic animal ecology will be illustrated.
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Derived from
20410487 Ecologia animale II in Biodiversità e gestione degli ecosistemi LM-6 VIGNOLI LEONARDO
( syllabus)
The study of animal communities and macroecology will be organized in different modules. (i) Co-occurrence: the co-occurrence module allows to test non-random models of co-occurrence of species in a presence-absence matrix; (ii) Macroecology: the study of the partitioning of physical space and ecological resources by species. Macro-ecological studies consist of the analysis of traits at the species level, such as body size, geographical area and average abundance, measured at large spatial scales; (iii) Niche overlap: the study of niche overlap has a long history in community ecology. Simple theories of of limiting similarity y and ecological character displacement predict that interspecific competition will lead to a reduction in the niche overlap of competing species. Testing this idea with empirical data leads to three questions: 1) which aspects of the niche should be measured? 2) How can the niche overlap between species pairs be quantified? 3) how much niche overlap would you expect in the absence of competition? (iv) Overlap of body size: this module allows to test for unusual patterns in the body sizes of coexisting species, and to compare those patterns to what might be expected in a random assemblage that was not structured by interspecific interactions; (v) Diversity of communities: the diversity of species is a central object of study both in the basic and applied ecology of communities. Two main issues will be addressed in the study of species diversity. The first is how can we quantify the diversity of an assemblage, and second, how we can compare statistically the diversity of two different assemblages; (vi) Functional groups or guilds: groups of species within a community that share common resources are considered guilds. This module will allow to incorporate the guild structure into community analyses. - Through simulations in the laboratory and in the field, the student will learn the dynamics that determine the organization and structure of some selected animal communities. - Interactive computer programs will be used for the analysis of null models in community ecology to test community models with experimental and non-experimental data. The Monte Carlo randomizations will be illustrated as an analytical method to compare real communities with "pseudo-communities" created using different algorithms. The wide applicability of null models in applied and basic animal ecology will be illustrated
( reference books)
NOTES AND POWER POINTS ARE PROVIDED BY THE TEACHER. THE FOLLOWING TEXTBOOKS HELP TO STUDY SOME ASPECTS OF THE PROGRAMME: - BEGON, M., TOWNSEND, C.R., HARPER, J.L.— ECOLOGY: FROM INDIVIDUALS TO ECOSYSTEMS / 4TH ED. BLACKWELL PUBLISHING, OXFORD UK. - BOITANI L. & FULLER T.K. (EDS.), 2000. RESEARCH TECHNIQUES IN ANIMAL ECOLOGY. CONTROVERSIES AND CONSEQUENCES. COLUMBIA UNIVERSITY PRESS, N.Y.; - GOTELLI, N.J. AND A.M. ELLISON. 2004. A PRIMER OF ECOLOGICAL STATISTICS. SINAUER ASSOCIATES, INC., SUNDERLAND, MA.; - GOTELLI, N.J. 2008. A PRIMER OF ECOLOGY. 4TH EDITION. SINAUER ASSOCIATES, INC., SUNDERLAND, MA. - HENDERSON, P.A. 2003. PRACTICAL METHODS IN ECOLOGY. BLACKWELL SCIENCE LTD - VERHOEF, H.F., MORIN, P.J. 2010. COMMUNITY ECOLOGY. PROCESSES, MODELS, AND APPLICATIONS. OXFORD UNIVERSITY PRESS, USA - MITTELBACH, G.G., MCGILL, B.J. 2019. COMMUNITY ECOLOGY. OXFORD UNIVERSITY PRESS, USA.
Students are provided with a document that lists for each lesson topic which textbook and which chapters are most relevant for the study, and for each topic it lists recent scientific papers for further study. Students are also provided with PDFs of lessons and of scientific papers suggested.
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6
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BIO/05
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40
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10
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Related or supplementary learning activities
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ITA |
20410553 -
Botanica di campo ed applicata
(objectives)
At the end of the course, the students will have learned the basic concepts on the main morpho-structural differences of plant organisms according to their adaptations to different environments. They will be able to identify and explain form-function relationships in the plants. They will be able to recognize the most representative and dominant species of the main plant coenoses of the Italian landscape. In the field of applied botany, the students will have discovered the many uses, traditional and modern, that plant species can have. They will know the use of plant species as climatic, edaphic and anthropic disturbance descriptors and as bioindicators of environmental quality and phytoremediation agents of contaminated waters and soils. They will know the issues related to alien plant species (introduction, ecosystem impact, management and control methodologies). Basic concepts of forensic botany will be acquired. Through laboratory and field exercises, the students will acquire techniques and methodologies to sample and identify plant species and analyze and know how to interpret the collected flora and vegetation data.
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Derived from
20410553 Botanica di campo ed applicata in Biodiversità e gestione degli ecosistemi LM-6 CESCHIN SIMONA
( syllabus)
Main morpho-structural differences of plant organisms according to their adaptations to different environments (mountain, coastal, lake, river, rock, wall, city). Examples of Form-Function relationship in the plants. Definition of the most representative and dominant species of the main plant coenoses of the Italian landscape. Techniques and methods of sampling, collection and identification of vascular plants. In the field of applied botany, a description of the many traditional and modern uses of plant heritage. Use of plant species as descriptors of climate, soil and human disturbance. Their use as bioindicators of environmental quality and as phytoremediation agents of contaminated waters and soils. Meaning and problems related to alien flora (introduction, ecosystem impact, management and control methodologies). Contribution of botany to forensic sciences: palynology, dendrology, dendrochronology, dating of plant remains, poisonous and drug plants. Laboratory and field exercises on the topics covered during the course.
( reference books)
Pdfs of the lessons performed during the course and the following book:
- Schauer T., Caspari C. 1997. Guida all'identificazione delle piante. Zanichelli. - Book of Sistematic Botany utilized in the course of Biodiversity of vascular plant: Judd, Campbell, Kellogg, Stevens, Donoghue. Botanica sistematica. Un approccio filogenetico. Piccin. OR Pasqua, Abbate, Forni. Botanica generale e Diversità vegetale. Piccin.
The teacher receives Mon, Wed, Fri from 12.00 to 13.00 by appointment via email: simona.ceschin@uniroma3.it
In the event of an extension of the health emergency from COVID-19, all the provisions governing the methods of receiving students will be implemented. In particular, the teacher will receive electronically by appointment via email: simona.ceschin@uniroma3.it
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6
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BIO/02
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32
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-
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20
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-
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Related or supplementary learning activities
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ITA |
20410527 -
Neurobiologia molecolare dello sviluppo
(objectives)
The Course aims to provide the student with advanced knowledge and skills in molecular biology of neurodevelopment, with particular reference to the central nervous system of vertebrates.
The aim of the first module is to provide the student with general knowledge about the cellular processes that lead, in the course of early and late embryogenesis, to the formation of the central nervous system from the neural tube. Advanced concepts on morphogenetic processes ensuring the achievement of the general cytoarchitecture of telecephalon, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord will be provided. In particular, the student will acquire knowledge and skills on neuronal migration processes (under normal and pathological conditions) that lead to the layering of the cerebral cortex, spinal cord, and cerebellum. Advanced knowledge and expertise will also be provided on the cellular processes underlying adult neurogenesis and neurodegeneration, focusing on the role of apoptosis and autophagy. The student, through an interactive teaching will acquire skills in the study of pathological conditions, such as the most common neurodegenerative disorders (Alzheimer's disease, Parkinson's disease, Huntington's chorea).
The aim of the second module is to provide the student with general knowledge regarding the gene expression underlying the differentiation and morphogenesis of the nervous tissue. These molecular mechanisms will be addressed in different study models. It will be highlighted how neurogenesis and plasticity have profound functional implications in the nervous system throughout the life of the individual. The student, by means an interactive teaching, will acquire skills on new biomolecular technologies to study the development and functionality of the nervous system under physiological and pathological conditions.
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MORENO SANDRA
( syllabus)
Overview of the development of multicellular organisms. Ectodermal derivation of neural tissue in invertebrates and vertebrates. Primary neurulation: cellular and molecular mechanisms of neural plate formation, neural folds, neural groove and neural tube. Secondary neurulation. Neural tube closure defects in mammals: genetic and environmental causes. Morphogenesis and differentiation of the brain and spinal cord. Role of cell death in central nervous system morphogenesis: apoptotic mechanisms. Layering of cerebral and cerebellar cortical structures. Congenital diseases related to neuronal migration defects: the case of peroxisomal disorders. Neural stem cells: neuronal and glial differentiation. Neurogenesis in the adult brain: the subventricular and subgranular areas of the mammalian dentate gyrus. Pathways regulating activity of the neurogenic niche. Neurogenesis and neurodegeneration in the senescent brain. Neurodegenerative diseases related to aging: common features and anatomical and genetic-molecular specificities. Protein aggregates, role of apoptosis and autophagy and therapies against neurodegeneration, based on stem cell transplants and endogenous regeneration.
( reference books)
Gilbert: "Developmental Biology", 9th Edition, Sinauer Associates
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CERVELLI MANUELA
( syllabus)
Neural induction. Polarity and segmentation. Determination and differentiation. The anteroposterior axis and the Hox genes. Growth and direction of axons. circRNA and long non-coding RNA in brain development. Role of regulatory RNAs in synaptic translation control. Role of polyamines in the modulation of glutamate receptors. Interaction between glutamatergic system, serotonergic system and polyamine metabolism. Animal models used in Alzheimer's disease.
( reference books)
Lo sviluppo del sistema nervoso. Authors: Dan H. Sanes; Thomas A. Reh; William A. Harris. Publishing house: Zanichelli.
Lecture slides will be provided.
The professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it
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3
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BIO/06
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20
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5
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-
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Attività formative affini ed integrative
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3
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BIO/11
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20
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-
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5
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-
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Attività formative affini ed integrative
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ITA |
20410594 -
L’Agenda 2030 delle Nazioni Unite per lo sviluppo sostenibile - Le implicazioni per le Scienze della Vita e della Terra
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Modulo di Base
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3
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20
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-
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Elective activities
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ITA |
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L’Agenda 2030 delle Nazioni Unite per lo sviluppo sostenibile - Le implicazioni per le Scienze della Vita e della Terra
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3
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GEO/03
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28
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-
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-
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Elective activities
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ITA |
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