Università Roma Tre

The course aims to provide students with the methodological principles of biochemical and bioinformatics techniques for protein analysis, through both a theoretical and practical approach. In detail, the course aims to provide basic skills on the principles of expression and purification of proteins, methods of quantification, analysis and characterization of the same by electrophoresis, chromatography and spectroscopy. The student will also acquire skills on the main methods of "omics" analysis (proteomics and interactomics). In addition, students will learn the theoretical principles and acquire technical skills related to the main bioinformatics methodologies for analysis and manipulation of protein sequences and structures. Finally, the course will allow students to learn how to plan and carry out a scientific experiment in biochemistry, and how to interpret and critically analyze the results obtained.