Università Roma Tre

The Bioenergetics and Metabolism module (Prof. Alessandra di Masi) will cover the following topics: bioenergetics and types of biochemical reactions, glycolysis, gluconeogenesis, pentose phosphate pathway, principles of metabolic regulation, citric acid cycle, fatty acid catabolism, amino acid oxidation and urea cycle, oxidative phosphorylation.

The practice module of the course of Biochemistry is NOT MANDATORY and includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Protein quantification by Bradford method.
2. Separation of proteins by SDS-PAGE method and determination of their molecular weights by migration distance.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
The results obtained will be analyzed and discussed together with the teacher during a frontal lesson in the classroom.

- NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
- VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI
- MCGRAW-HILLMORAN ET AL., BIOCHIMICA, ZANICHELLI.
- M. K. Campbell, S. O. Farrell, O. M. McDougal "Biochimica", Ed. Edises


RECEPTION TIME: Monday 10 am

First module: Protein Structure

Water
Weak interactions in aqueous systems
The hydrogen bond
Ionisation of water, weak acids and bases
Amino acids, peptides and proteins
Amino acids
Peptides and proteins
The covalent bonds of peptides and proteins
The structure of proteins
Primary structure
Secondary structure
Tertiary structure
Quaternary structure
Supramolecular structures
Folding and unfolding (general aspects)
Protein functions
Enzymology
Carbohydrates
Lipids
Nucleotides and nucleic acids
Techniques for the isolation and characterization of proteins

Nelson, Cox – Lehninger Principles of Biochemistry, W.H. Freeman, 8th edition

The practical module of the course includes three laboratory experiences. The topics of the experiences are:

1. Protein quantification by Bradford method.

2. Separation of proteins by SDS-PAGE method and determination of their molecular weights by migration distance.

3. Characterization of the enzymatic activity of lactate dehydrogenase.

The slides of each lecture will be made available