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Derived from
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20410221 BIOCHIMICA in Biological sciences L-13 R DI MASI ALESSANDRA, POLTICELLI FABIO, DE SIMONE GIOVANNA
(syllabus)
The Bioenergetics and Metabolism module (Prof. Alessandra di Masi) will cover the following topics: bioenergetics and types of biochemical reactions, glycolysis, gluconeogenesis, pentose phosphate pathway, principles of metabolic regulation, citric acid cycle, fatty acid catabolism, amino acid oxidation and urea cycle, oxidative phosphorylation.
The practice module of the course of Biochemistry is NOT MANDATORY and includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Protein quantification by Bradford method.
2. Separation of proteins by SDS-PAGE method and determination of their molecular weights by migration distance.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
The results obtained will be analyzed and discussed together with the teacher during a frontal lesson in the classroom.
(reference books)
- NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
- VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI
- MCGRAW-HILLMORAN ET AL., BIOCHIMICA, ZANICHELLI.
- M. K. Campbell, S. O. Farrell, O. M. McDougal "Biochimica", Ed. Edises
RECEPTION TIME: Monday 10 am
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Università Roma Tre