INTEGRATED COURSE OF BIOCHEMISTRY, MOLECULAR BIOLOGY AND MICROBIOLOGY
(objectives)
The module of General and Environmental Microbiology aims to provide the basic principles of the structure, function and evolution of microbial cells, with particular regard to bacteria. The knowledge and skills acquired in this module will represent a reference framework for understanding the role of microorganisms in natural and anthropic environments and for the study of the biotechnological applications of microorganisms for environmental protection and sustainability. The module aims at the acquisition of Cultural Competences, i.e. basic knowledge, on (i) the structural, functional and metabolic diversity present in the microbial world, (ii) the mechanisms responsible for the structure and the functioning of bacterial cells, (iii) the processes driving the evolution of bacterial species, (iv) the structure, diversity and life cycles of viruses, (v) the methods and approaches for the study of microbial communities, (vi) the role and effects of microorganisms in the environment. The module also aims at the acquisition of Methodological Competences, i.e. the understanding, through both theoretical lessons and practical activities, of the scientific bases and methodological approaches for the study of bacteria in natural environments and in the laboratory, and for the characterization of complex microbial populations by culture and molecular techniques. A further aim is also the acquisition of the ability to interpret microbiological data and analyse the scientific literature to obtain information on the structure and function of microorganisms, with the final goal to understand natural or artificial events involving microorganisms. The second module of the course: Elements of Biochemistry aims to provide the basic notions related to the structure and function of biological macromolecules, enzymatic catalysis and cell metabolism. These notions are necessary for the continuation of studies on the functioning of eukaryotic and prokaryotic organisms in the context of the Degree Course. The Educational Objectives are the acquisition of cultural skills, i.e. basic knowledge about the structure and function of biological macromolecules (nucleic acids, proteins, carbohydrates, lipids) and the mechanisms that regulate the synthesis and degradation of biological macromolecules. Furthermore, the module aims to provide basic knowledge on the mechanisms by which genetic information is conserved, transmitted and expressed properties. The Educational Objectives are also the acquisition of Methodological Competences, or the acquisition, through practical laboratory activity, of the scientific and methodological bases of the most important experimental techniques in the field of Biochemistry and Molecular Biology and the acquisition, through the elaboration and display of individual papers, of the ability to find in the scientific literature information on the structure and function of biological macromolecules capable of contextualizing, analyzing and critically interpreting natural or artificial events involving biological macromolecules.
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Code
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20410540 |
Language
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ITA |
Type of certificate
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Profit certificate
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Module: ELEMENTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
(objectives)
The second module of the course: Elements of Biochemistry aims to provide the basic notions related to the structure and function of biological macromolecules, enzymatic catalysis and cell metabolism. These notions are necessary for the continuation of studies on the functioning of eukaryotic and prokaryotic organisms in the context of the Degree Course. The Educational Objectives are the acquisition of cultural skills, i.e. basic knowledge about the structure and function of biological macromolecules (nucleic acids, proteins, carbohydrates, lipids) and the mechanisms that regulate the synthesis and degradation of biological macromolecules. Furthermore, the module aims to provide basic knowledge on the mechanisms by which genetic information is conserved, transmitted and expressed properties. The Educational Objectives are also the acquisition of Methodological Competences, or the acquisition, through practical laboratory activity, of the scientific and methodological bases of the most important experimental techniques in the field of Biochemistry and Molecular Biology and the acquisition, through the elaboration and display of individual papers, of the ability to find in the scientific literature information on the structure and function of biological macromolecules capable of contextualizing, analyzing and critically interpreting natural or artificial events involving biological macromolecules.
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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3
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Scientific Disciplinary Sector Code
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BIO/10
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Contact Hours
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24
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Credits
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3
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Scientific Disciplinary Sector Code
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BIO/11
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Contact Hours
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24
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Teacher
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DI MASI ALESSANDRA
(syllabus)
PROGRAM OF THE MODULE OF BIOCHEMISTRY - Prof. Alessandra di Masi Water: physical and chemical properties. Proteins: amino acids, peptide bond, primary structure, secondary structure, tertiary structure and quaternary structure, examples of structure-function relationships. Carbohydrates: monosaccharides, polysaccharides, glycoproteins. Lipids: classification of lipids, lipid bilayer. Enzymes and enzymatic catalysis: general properties of enzymes, the concept of active site and specificity, mechanisms of catalysis, Michaelis-Menten equation, enzymatic inhibition, control of enzymatic activity. Cellular bioenergetics: anabolism and catabolism, transfer of phosphoric groups and ATP, biological redox reactions. Outline of the metabolism of carbohydrates, lipids, and proteins.
(reference books)
TEXTS ADOPTED IN THE MODULE OF "ELEMENTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY - D.L. Nelson and M.M. Cox, “Introduction to Lehninger's Biochemistry”, Ed Zanichelli - D. Voet, J.D. Voet, C.W. Pratt, “Principles of Biochemistry”, Ed Zanichelli. - M. K. Campbell, S. O. Farrell, O. M. McDougal "Biochimica", Ed. Edises
Lessons will be also provided.
Prof. Alessandra di Masi receives by appointment on Mondays from 9 to 10 am. Please fix an appointment by email (alessandra.dimasi@uniroma3.it).
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
At a distance
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Attendance
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not mandatory
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Teacher
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GALLO VALENTINA
(syllabus)
The Course aims to provide students with basic knowledge relating to biochemistry and cellular metabolism. Carbohydrates, Lipids, Amino acids, proteins: structure of proteins and examples of structure-function relationships. Enzymes and enzymatic catalysis: Definition of active site and substrate specificity; the Michaelis-Menten equation, enzyme inhibition. Introduction to aerobic and anaerobic metabolism. Metabolism of carbohydrates, lipids and nitrogen compounds. Energy production in mitochondria.
The Course also aims to provide basic knowledge of molecular biology. Purine and pyrimidine nucleotides; structure of biological macromolecules; structure of eukaryotic chromosomes; Replication, Transcription; Translation; Recombinant DNA and Genetically modified organisms.
(reference books)
Nelson, Cox Introduzione alla biochimica di Lehninger - Zanichelli
Stefani-Taddei, Chimica, Biochimica e Biologia Applicata- Zanichelli
Chimica e Biochimica, a cura di Samaja e Paroni - Piccin
Elementi di Biologia e Genetica, a cura di Raven et al. - Piccin
Lecture notes and calculation exercises will be provided.
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Dates of beginning and end of teaching activities
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From to |
Attendance
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not mandatory
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Evaluation methods
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Written test
Oral exam
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Module: GENERAL AND ENVIRONMENTAL MICROBIOLOGY
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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6
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Scientific Disciplinary Sector Code
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BIO/19
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Contact Hours
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40
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Laboratory Hours
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10
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Type of Activity
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Core compulsory activities
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