Università Roma Tre

To learn how to acquire, analyze and critically evaluate experimental data related to: interaction between receptors and ligands, enzymatic activity, optical spectroscopy
The programs is divided into:
1) Study of the interaction between receptors and ligands: methods at equilibrium and transient kinetics:
a) At equilibrium (thermodynamic parameters: Kd: dissociation constant at equilibrium, cooperativity, number of binding sites)
b) Rapid kinetics (kinetic parameters: k: velocity constants)
2) Study of enzymatic activity: steady state kinetics
a) In Steady State (when [ES] is almost constant during experimental observation)
b) In the presence of inhibitors
3) Time-resolved optical spectroscopy and complex kinetics
4) Absorption spectroscopy, fluorescence and circular dichroism

Recommended textbooks:
M. C. Bonaccorsi di Patti et al. Metodologie Biochimiche - Casa Editrice Ambrosiana
Ninfa/Ballou Metodologie di base per la Biochimica e la Biotecnologia - ed. Zanichelli
Reed et al. Metodologie di Base per le Scienze Biomolecolari- ed. Zanichelli
P. Silvestroni, Fondamenti di chimica - Casa Editrice Ambrosiana
D.M. Etter Introduction to MATLAB 7– Pearson Education, Inc.

Software to use
EXCEL (Office) or CALC (Open Office) * (for linear methods)
MATLAB (Mathworks) or OCTAVE (GNU Octave) * (for spectral analysis and non-linear methods)
GEPASI (P. Mendes et al.) * Or COPASI (Copasi dev. Group) * (for complex reactions)
(*) indicates free software
Lecture notes and calculation exercises will be provided

The professor receives every day from 8.00 to 9.00 by appointment via e-mail: giovanni.antonini@uniroma3.it