Università Roma Tre

Cultural knowledge: the aim of this course is to deepen the knowledge in cell biology, with special emphasis to the structural organization and to the functions of different compartments of eukaryotic animals cells. the course is aimed at providing students with the cultural and methodological grounding that allow the critical discussion of the scientific literature in the field of cell biology. a laboratory activity will be organized to promote hands-on experience with various "state of the art" techniques used in modern research.
Methodological skills: to know how to design an experiment, to acquire and analyze the data obtained from the laboratory activity.
Topics:
the course will deepen the following topics: the compartmentalization of cells; the sorting and the transport of proteins in different cell compartments; the intracellular vesicular traffic; the maintenance of the compartmental diversity; endocytosis and exocytosis, extracellular vesicles; structures and mechanisms involved in protein and organelle degradation: autophagy; the cytoskeleton organization with special emphasis to cell behavior (cell adhesion and migration).
The laboratory activity will be mainly devoted to the preparation and maintenance of cell cultures and to the design of a scientific experiment by students.

1) ALBERTS BRUCE , JOHNSON ALEXANDER , LEWIS JULIAN , RAFF MARTIN , ROBERTS KEITH , WALTER PETER. “BIOLOGIA MOLECOLARE DELLA CELLULA.” 6TH ED., ZANICHELLI
2) LODISH HARVEY , BERK ARNOLD , ZIPURSKY SLAWRENCE , MATSUDAIRA PAUL , BALTIMORE DAVID , DARNELL JAMES E. “BIOLOGIA MOLECOLARE DELLA CELLULA.” ZANICHELLI THIRD. ITALIAN EDITION, CARRIED OUT ON THE SIXTH AMERICAN EDITION.
SLIDES AND SCIENTIFIC PAPERS PROVIDED BY THE TEACHER.

Slides in PDF format of all the lectures will be provided by the teacher.

The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it