Università Roma Tre

STRUCTURE-FUNCTION RELATIONSHIPS OF PROTEINS. FROM AMINOACIDS to MYOGLOBIN AND HEMOGLOBIN.
STRUCTURAL AND FUNCTIONAL PROPERTIES OF THE ENZYMES. FROM THE MICHAELIS-MENTEN EQUATION TO THE PRE-STEADY-STATE AND THE INHIBITION MECHANISMS.
MOST IMPORTANT CYCLES AND METABOLIC PATHWAYS OF CARBS, LIPIDS AND PROTEINS.

RECEPTION: MONDAY, FROM 3 TO 4 AM.

MCKEE, BIOCHIMICA, MCGRAW - HILL
NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI
STRYER, BIOCHIMICA, ZANICHELLI
RAWN, BIOCHIMICA MCGRAW-HILL
MORAN ET AL., BIOCHIMICA MCGRAW-HILL
GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI

The Bioenergetics and Metabolism module (Prof. Alessandra di Masi) will cover the following topics: bioenergetics and types of biochemical reactions, glycolysis, gluconeogenesis, pentose phosphate pathway, principles of metabolic regulation, citric acid cycle, fatty acid catabolism, amino acid oxidation and urea cycle, oxidative phosphorylation.

The practice module of the course of Biochemistry includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.
2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.
3. Characterization of the enzymatic activity of lactate dehydrogenase.

MCKEE, BIOCHIMICA, MCGRAW - HILL. ALTRI TESTI: NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI RAWN, BIOCHIMICA, MCGRAW-HILLMORAN ET AL., BIOCHIMICA, MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI


RECEPTION TIME: Monday 10 am

First module: Protein Structure

Water
Weak interactions in aqueous systems
The hydrogen bond
Ionisation of water, weak acids and bases
Amino acids, peptides and proteins
Amino acids
Peptides and proteins
The covalent bonds of peptides and proteins
The structure of proteins
Primary structure
Secondary structure
Tertiary structure
Quaternary structure
Supramolecular structures
Folding and unfolding (general aspects)

Nelson, Cox – Lehninger Principles of Biochemistry, W.H. Freeman, 8th edition

The practice module of the course of Biochemistry includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:

1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.

2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.

3. Characterization of the enzymatic activity of lactate dehydrogenase.

MCKEE, BIOCHIMICA, MCGRAW - HILL.
NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI
STRYER, BIOCHIMICA, ZANICHELLI
RAWN, BIOCHIMICA, MCGRAW-HILLMORAN ET AL., BIOCHIMICA, MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI