INTEGRATED COURSE OF BIOCHEMISTRY, MOLECULAR BIOLOGY AND MICROBIOLOGY
(objectives)
The module of General and Environmental Microbiology aims to provide the basic principles of the structure, function and evolution of microbial cells, with particular regard to bacteria. The knowledge and skills acquired in this module will represent a reference framework for understanding the role of microorganisms in natural and anthropic environments and for the study of the biotechnological applications of microorganisms for environmental protection and sustainability. The module aims at the acquisition of Cultural Competences, i.e. basic knowledge, on (i) the structural, functional and metabolic diversity present in the microbial world, (ii) the mechanisms responsible for the structure and the functioning of bacterial cells, (iii) the processes driving the evolution of bacterial species, (iv) the structure, diversity and life cycles of viruses, (v) the methods and approaches for the study of microbial communities, (vi) the role and effects of microorganisms in the environment. The module also aims at the acquisition of Methodological Competences, i.e. the understanding, through both theoretical lessons and practical activities, of the scientific bases and methodological approaches for the study of bacteria in natural environments and in the laboratory, and for the characterization of complex microbial populations by culture and molecular techniques. A further aim is also the acquisition of the ability to interpret microbiological data and analyse the scientific literature to obtain information on the structure and function of microorganisms, with the final goal to understand natural or artificial events involving microorganisms. The second module of the course: Elements of Biochemistry aims to provide the basic notions related to the structure and function of biological macromolecules, enzymatic catalysis and cell metabolism. These notions are necessary for the continuation of studies on the functioning of eukaryotic and prokaryotic organisms in the context of the Degree Course. The Educational Objectives are the acquisition of cultural skills, i.e. basic knowledge about the structure and function of biological macromolecules (nucleic acids, proteins, carbohydrates, lipids) and the mechanisms that regulate the synthesis and degradation of biological macromolecules. Furthermore, the module aims to provide basic knowledge on the mechanisms by which genetic information is conserved, transmitted and expressed properties. The Educational Objectives are also the acquisition of Methodological Competences, or the acquisition, through practical laboratory activity, of the scientific and methodological bases of the most important experimental techniques in the field of Biochemistry and Molecular Biology and the acquisition, through the elaboration and display of individual papers, of the ability to find in the scientific literature information on the structure and function of biological macromolecules capable of contextualizing, analyzing and critically interpreting natural or artificial events involving biological macromolecules.
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Code
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20410540 |
Language
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ITA |
Type of certificate
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Profit certificate
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Module: ELEMENTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
(objectives)
The second module of the course: Elements of Biochemistry aims to provide the basic notions related to the structure and function of biological macromolecules, enzymatic catalysis and cell metabolism. These notions are necessary for the continuation of studies on the functioning of eukaryotic and prokaryotic organisms in the context of the Degree Course. The Educational Objectives are the acquisition of cultural skills, i.e. basic knowledge about the structure and function of biological macromolecules (nucleic acids, proteins, carbohydrates, lipids) and the mechanisms that regulate the synthesis and degradation of biological macromolecules. Furthermore, the module aims to provide basic knowledge on the mechanisms by which genetic information is conserved, transmitted and expressed properties. The Educational Objectives are also the acquisition of Methodological Competences, or the acquisition, through practical laboratory activity, of the scientific and methodological bases of the most important experimental techniques in the field of Biochemistry and Molecular Biology and the acquisition, through the elaboration and display of individual papers, of the ability to find in the scientific literature information on the structure and function of biological macromolecules capable of contextualizing, analyzing and critically interpreting natural or artificial events involving biological macromolecules.
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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3
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Scientific Disciplinary Sector Code
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BIO/10
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Contact Hours
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24
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Credits
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3
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Scientific Disciplinary Sector Code
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BIO/11
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Contact Hours
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24
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Teacher
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DI MASI ALESSANDRA
(syllabus)
PROGRAM OF THE MODULE OF BIOCHEMISTRY - Prof. Alessandra di Masi Water: physical and chemical properties. Proteins: amino acids, peptide bond, primary structure, secondary structure, tertiary structure and quaternary structure, examples of structure-function relationships. Carbohydrates: monosaccharides, polysaccharides, glycoproteins. Lipids: classification of lipids, lipid bilayer. Enzymes and enzymatic catalysis: general properties of enzymes, the concept of active site and specificity, mechanisms of catalysis, Michaelis-Menten equation, enzymatic inhibition, control of enzymatic activity. Cellular bioenergetics: anabolism and catabolism, transfer of phosphoric groups and ATP, biological redox reactions. Outline of the metabolism of carbohydrates, lipids, and proteins.
(reference books)
TEXTS ADOPTED IN THE MODULE OF "ELEMENTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY - D.L. Nelson and M.M. Cox, “Introduction to Lehninger's Biochemistry”, Ed Zanichelli - D. Voet, J.D. Voet, C.W. Pratt, “Principles of Biochemistry”, Ed Zanichelli.
Lessons will be also provided.
Prof. Alessandra di Masi receives by appointment on Mondays from 9 to 10 am. Please fix an appointment by email (alessandra.dimasi@uniroma3.it).
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
At a distance
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Attendance
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not mandatory
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Teacher
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ANTONINI GIOVANNI
(syllabus)
The Course aims to provide basic knowledge of molecular biology. Purine and pyrimidine nucleotides; structure of biological macromolecules; structure of eukaryotic chromosomes; Replication, Transcription; Translation; Recombinant DNA and Genetically modified organisms.
(reference books)
Nelson, Cox Introduzione alla biochimica di Lehninger - Zanichelli Stefani-Taddei, Chimica, Biochimica e Biologia Applicata- Zanichelli Chimica e Biochimica, a cura di Samaja e Paroni - Piccin Elementi di Biologia e Genetica, a cura di Raven et al. - Piccin
Lecture notes and calculation exercises will be provided Professors receive every day by appointment by e-mail: alessandra.dimasi@uniroma3.it or giovanni.antonini@uniroma3.it
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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not mandatory
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Module: GENERAL AND ENVIRONMENTAL MICROBIOLOGY
(objectives)
The module of General and Environmental Microbiology aims to provide the basic principles of the structure, function and evolution of microbial cells, with particular regard to bacteria. The knowledge and skills acquired in this module will represent a reference framework for understanding the role of microorganisms in natural and anthropic environments and for the study of the biotechnological applications of microorganisms for environmental protection and sustainability. The module aims at the acquisition of Cultural Competences, i.e. basic knowledge, on (i) the structural, functional and metabolic diversity present in the microbial world, (ii) the mechanisms responsible for the structure and the functioning of bacterial cells, (iii) the processes driving the evolution of bacterial species, (iv) the structure, diversity and life cycles of viruses, (v) the methods and approaches for the study of microbial communities, (vi) the role and effects of microorganisms in the environment. The module also aims at the acquisition of Methodological Competences, i.e. the understanding, through both theoretical lessons and practical activities, of the scientific bases and methodological approaches for the study of bacteria in natural environments and in the laboratory, and for the characterization of complex microbial populations by culture and molecular techniques. A further aim is also the acquisition of the ability to interpret microbiological data and analyse the scientific literature to obtain information on the structure and function of microorganisms, with the final goal to understand natural or artificial events involving microorganisms.
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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6
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Scientific Disciplinary Sector Code
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BIO/19
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Contact Hours
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40
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Laboratory Hours
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10
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Type of Activity
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Core compulsory activities
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Teacher
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IMPERI FRANCESCO
(syllabus)
Introduction to and history of Microbiology Functional diversity and distribution of microorganisms, main discoveries in the microbiology field, examples of biotechnological applications of microorganisms.
Structure and functions of bacterial cells Structure of the prokaryotic cell. Cell division: binary fission. Cytoplasm, cytoplasmic inclusions and sub-cellular organelles. Cytoplasmic membrane and cell wall in Bacteria and Archaea. Protein secretion and cell wall biogenesis. Surface appendages: flagella and pili. Bacterial motility. Cell differentiation in bacteria and sporulation. Microbial biofilms. Bacterial metabolism: chemoorganotrophs, chemolithotrophs and phototrophs. Bacterial cultures and methods for bacterial cell count. Antibiotics: activity, mechanisms of action, evolution of resistance. Transcriptional and post-transcriptional regulation of gene expression in bacteria.
Elements of bacterial genetics Structure of the bacterial chromosome. Operons. Genome and pangenome. Mobile genetic elements: plasmids and transposable elements. Horizontal gene transfer (HGT): transformation, conjugation, transduction. Role of mutations and HGT in microbial evolution.
Principles of virology Structure of viruses and infection strategies. Diversity and replication of viral genomes. Examples of viral gene regulation. Diffusion and role of virus in the environment.
Principles of bacterial taxonomy and environmental microbiology The concept of bacterial species. Main prokaryotic groups. Bacterial identification: culture- and molecular-based approaches. Molecular clocks and phylogenetic analysis. Characterization of complex microbial communities. Metagenomic analysis and unculturable bacteria. Microbial ecosystems. Interaction of microorganisms with plants, animals and other microorganisms. Role of microorganisms in biogeochemical cycling.
(reference books)
Title: Brock. Biology of microorganisms. Authors: Michael T. Madigan - John M. Martinko - David A. Stahl - Kelly S. Bender - Daniel H. Buckley. Editor: Pearson.
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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not mandatory
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Teacher
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VISAGGIO DANIELA
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Dates of beginning and end of teaching activities
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From to |
Attendance
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not mandatory
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Teacher
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CHEBBI ALIF
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Dates of beginning and end of teaching activities
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From to |
Attendance
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not mandatory
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