Università Roma Tre

1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.

Recommended textbooks:
1. METODOLOGIE BIOCHIMICHE STRUMENTI E TECNICHE PER IL LABORATORIO DEL NUOVO MILLENNIO, MACCARRONE M., E BIOMOLECOLARI, CASA EDITRICE ZANICHELLI
2. DAI GENI AI GENOMI, JEREMY W. DALE, MALCOLM VON SCHANTZ, NICK PLANT, A CURA DI: E. GINELLI, CASA EDITRICE EDISES
3. ANALISI DEI GENI E GENOMI, REECE, EDISES.
4. TECNICHE E METODI PER LA BIOLOGIA MOLECOLARE, AMALDI F., BENEDETTI P., PESOLE G., PLEVANI P., CASA EDITRICE AMBROSIANA.

Lecture notes and calculation exercises will be provided.
Non-attending students are invited to request information on the program, teaching materials and how to evaluate the profit.
The professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it

THE COURSE OF MOLECULAR BIOLOGY IS PART OF THE TEACHING ACTIVITIES OF THE DEGREE COURSE IN LIFE SCIENCES. THE COURSE THROUGH FRONTAL LESSONS AND LABORATORY TRAINING AIMS TO PROVIDE THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. TOOLS WILL BE GIVEN TO UNDERSTAND THE MAIN MOLECULAR BIOLOGY STRATEGIES AND TECHNIQUES UTILIZED TO STUDY GENES AND GENOMES. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE STUDENTS WILL GET ACQUAINTED WITH THEORETICAL KNOWLEDGES AND METHODOLOGICAL SKILLS THAT WILL ENABLE THEM TO APPLY THE CORRECT STUDY TO SOLVE SPECIFIC PROBLEMS IN THE FIELD OF MOLECULAR BIOLOGY.
THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; DNA REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI

The professor receives every day from 8.00 to 9.00 by appointment via e-mail: giovanni.antonini@uniroma3.it