Università Roma Tre

Water and its properties
Weak interactions in aqueous solutions
The hydrogen bond
Dissociation equilibria, weak acids and bases
Amino acids, peptides and proteins
Proteins' primary, secondary, tertiary, and quaternary structure
Supramolecular structures
Protein folding and unfolding

Nelson, Cox - I principi di Biochimica del Lehninger. Zanichelli 2018

Garrett, Grisham –Biochimica, Piccin, 2014

Berg, Tymoczko, Stryer – Biochimica, Zanichelli, 2012

McKee e McKee – Biochimica, McGraw Hill, 2006

The practice module of the course of Biochemistry includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.
2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.
3. Characterization of the enzymatic activity of lactate dehydrogenase.

MCKEE, BIOCHIMICA, MCGRAW - HILL. ALTRI TESTI: NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI RAWN, BIOCHIMICA, MCGRAW-HILLMORAN ET AL., BIOCHIMICA, MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI


RECEPTION TIME: Monday 10 am