Università Roma Tre

Practical laboratories
Determination of amine oxidase enzyme activity and hydrogen peroxide production through spectrophotometric methods on maize and chickpea plant extracts.
Observation under light microscopy of tissue-specific gene expression of copper amine oxidases (AtCuAO) after GUS-staining using 5-day-old PromAtCuAO-GUS-GFP Arabidopsis transgenic seedlings, fixed in 90% acetone and washed in phosphate buffer.

Introductory material and experimental procedure provided by the teacher

The Prof. receives every day by appointment via email: alessandra.cona@uniroma3.it

SIGNAL PERCEPTION AND TRANSDUCTION PATHWAYS IN PLANT CELLS: RECEPTORS, MEDIATORS AND MODALITY OF TRANSDUCTION. SIGNALING PATHWAYS UPON HORMONE OR LIGHT PERCEPTION. CELL WALL AT THE INTERFACE BETWEEN INTRACELLULAR AND EXTRACELLULAR ENVIRONMENT: CELL SURFACE SENSORS AND RECEPTORS. ROLE OF REACTIVE OXYGEN SPECIES IN SIGNAL TRANSDUCTION. PHENOTYPIC PLASTICITY IN RESPONSES TO ABIOTIC STRESSES (DROUGHT, FLOODING, SALT STRESS, HIGH AND LOW TEMPERATURE, HIGH LIGHT). SECONDARY METABOLISM AND DEFENCE STRATEGY AGAINST WOUNDING OR PATHOGEN ATTACK. INNATE IMMUNITY AND EFFECTOR TRIGGERED IMMUNITY. LOCAL AND SYSTEMIC DEFENCE RESPONSES. PLANT COMPOUNDS AS SOURCE OF DRUGS, NUTRACEUTICAL FOOD OR NATURAL HERBICIDES. BASIC KNOWLEDGE IN PHYTOREMEDIATION.

SCIENTIFIC ARTICLES WILL BE PROVIDED BY THE TEACHER AND DISCUSSED AT LESSONS.

The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it