Università Roma Tre

MODULE 3 PRINCIPLES OF VIROLOGY AND ANTIMICROBIAL IMMUNITY
INTRODUCTION TO VIROLOGY. ORIGIN OF VIRUSES AND THEIR STRUCTURE THE VIRAL LIFE CYCLE. THE BALTIMORE SCHEME OF VIRUS CLASSIFICATION. GENOME VARIABILITY, THE CONCEPT OF VIRAL SPECIES AND THE DEFINITION OF QUASI-SPECIES. METHODS FOR GENOTYPING AND SEROTYPING. DEFINITION OF ACUTE, ABORTIVE, LATENT AND PERSISTENT INFECTION. REPLICATION STRATEGIES OF SPECIFIC VIRUSES THAT INFECT BACTERIA OR VERTEBRATE HOSTS WILL BE DESCRIBED TO GAIN INSIGHT INTO VIRAL EVOLUTION, VIRUS-CELL INTERACTIONS, PATHOGENESIS, HOST DEFENSE MECHANISMS, STRATEGIES OF VIRAL IMMUNOEVASIONS, METHODS OF VIRAL DETECTION, IDENTIFICATION AND TITRATION, ANTIVIRAL DRUGS AND PROFILAXIS OF VIRAL INFECTIONS (BACTERIOPHAGES: T4, T7, Λ, Q OR MS2, ΦX174 AND M13; ANIMAL VIRUSES: POLIOVIRUS, HEPATITIS A, B AND C VIRUSES, VESICULAR STOMATITIS VIRUS, INFLUENZA VIRUS, RETROVIRUSES AND AIDS VIRUS, SV40 AND PAPILLOMAVIRUS).
PRACTICAL EXERCISE WILL BE PERFORMED DURING THE COURSE.

BROCK, BIOLOGY OF MICROORGANISMS, EDS: M. T. MADIGAN - J. M. MARTINKO - D. A. STAHL - D. P. CLARK WEB SITES: WWW.MICROBIOLOGYBYTES.COM (CLICK ON SITEMAP AND USE ALSO THE LABWORK: ONLINE EXPERIMENTS ); HTTP://WWW.MICROBEWORLD.ORG/MICROBES/; HTTP://GSBS.UTMB.EDU/MICROBOOK/TOC.HTM; HTTP://WWW.BACT.WISC.EDU/MICROTEXTBOOK/
OTHER RECOMMENDED TEXTBOOKS (AVAILABLE IN THE ROMA TRE UNIVERSITY LIBRARY)
- PRESCOTT/HARLEY/KLEIN'S, MICROBIOLOGY
- MURRAY, ROSENTHAL, PFALLER, MEDICAL MICROBIOLOGY,
- CANN ALAN J.,PRINCIPLES OF MOLECULAR VIROLOGY,
- EDWARD K. WAGNER E MARTINEZ J. HEWLETT – BASIC VIROLOGY –BLACKWELL SCIENCE
TEACHERS WILL ALSO PROVIDE TECHNICAL AND EXPERIMENTAL PROTOCOLS FOR THE LABORATORY EXERCISES.

MODULE 3 PRINCIPLES OF VIROLOGY AND ANTIMICROBIAL IMMUNITY
INTRODUCTION TO VIROLOGY. ORIGIN OF VIRUSES AND THEIR STRUCTURE THE VIRAL LIFE CYCLE. THE BALTIMORE SCHEME OF VIRUS CLASSIFICATION. GENOME VARIABILITY, THE CONCEPT OF VIRAL SPECIES AND THE DEFINITION OF QUASI-SPECIES. METHODS FOR GENOTYPING AND SEROTYPING. DEFINITION OF ACUTE, ABORTIVE, LATENT AND PERSISTENT INFECTION. REPLICATION STRATEGIES OF SPECIFIC VIRUSES THAT INFECT BACTERIA OR VERTEBRATE HOSTS WILL BE DESCRIBED TO GAIN INSIGHT INTO VIRAL EVOLUTION, VIRUS-CELL INTERACTIONS, PATHOGENESIS, HOST DEFENSE MECHANISMS, STRATEGIES OF VIRAL IMMUNOEVASIONS, METHODS OF VIRAL DETECTION, IDENTIFICATION AND TITRATION, ANTIVIRAL DRUGS AND PROFILAXIS OF VIRAL INFECTIONS (BACTERIOPHAGES: T4, T7, Λ, Q OR MS2, ΦX174 AND M13; ANIMAL VIRUSES: POLIOVIRUS, HEPATITIS A, B AND C VIRUSES, VESICULAR STOMATITIS VIRUS, INFLUENZA VIRUS, RETROVIRUSES AND AIDS VIRUS, SV40 AND PAPILLOMAVIRUS).
PRACTICAL EXERCISE WILL BE PERFORMED DURING THE COURSE.

BROCK, BIOLOGY OF MICROORGANISMS, EDS: M. T. MADIGAN - J. M. MARTINKO - D. A. STAHL - D. P. CLARK + CANN ALAN J.,PRINCIPLES OF MOLECULAR VIROLOGY
WEB SITES: WWW.MICROBIOLOGYBYTES.COM (CLICK ON SITEMAP AND USE ALSO THE LABWORK: ONLINE EXPERIMENTS ); HTTP://WWW.MICROBEWORLD.ORG/MICROBES/; HTTP://GSBS.UTMB.EDU/MICROBOOK/TOC.HTM; HTTP://WWW.BACT.WISC.EDU/MICROTEXTBOOK/
OTHER RECOMMENDED TEXTBOOKS (AVAILABLE IN THE ROMA TRE UNIVERSITY LIBRARY)
- PRESCOTT/HARLEY/KLEIN'S, MICROBIOLOGY
- MURRAY, ROSENTHAL, PFALLER, MEDICAL MICROBIOLOGY,
- EDWARD K. WAGNER E MARTINEZ J. HEWLETT – BASIC VIROLOGY –BLACKWELL SCIENCE
TEACHERS WILL ALSO PROVIDE TECHNICAL AND EXPERIMENTAL PROTOCOLS FOR THE LABORATORY EXERCISES.

MODULE 2 BACTERIAL GENETICS
PLASMIDS. THE GENETIC ORGANIZATION OF PLASMIDS. PLASMID REPLICATION, COPY NUMBER AND PARTITION CONTROL. TRANSFORMATION. NATURAL AND ARTIFICIAL TRANSFORMATION. PHASES OF TRANSFORMATION. COMPETENCE. TRANSFORMATION IN GRAM-NEGATIVE BACTERIA. CONJUGATION. THE DISCOVERY OF F+ AND HFR E. COLI STRAINS. POLARITY OF CONJUGAL TRANSFER. CONJUGATION-BASED GENETIC MAPS. ORIGIN OH HFR STRAINS. F’ AND GENERATION OF F’ PLASMIDS. TRANSDUCTION. VIRULENT AND TEMPERATE BACTERIOPHAGES. GENERATION OF TRANSDUCING PHAGES AND DNA PACKAGING. GENETIC MAPS GENERATED BY TRANSDUCTION. •TRANSPOSITION AND TRANSPOSABLE ELEMENTS. MECHANISMS OF TRANSPOSITION•. PLASMIDS AND PHAGES AS DNA CLONING VEHICLES. • RESTRICTION ENZYMES. GENETIC ENGINEERING.

BROCK, BIOLOGY OF MICROORGANISMS, EDS: M. T. MADIGAN - J. M. MARTINKO - D. A. STAHL - D. P. CLARK + CANN ALAN J.,PRINCIPLES OF MOLECULAR VIROLOGY
WEB SITES: WWW.MICROBIOLOGYBYTES.COM (CLICK ON SITEMAP AND USE ALSO THE LABWORK: ONLINE EXPERIMENTS ); HTTP://WWW.MICROBEWORLD.ORG/MICROBES/; HTTP://GSBS.UTMB.EDU/MICROBOOK/TOC.HTM; HTTP://WWW.BACT.WISC.EDU/MICROTEXTBOOK/
OTHER RECOMMENDED TEXTBOOKS (AVAILABLE IN THE ROMA TRE UNIVERSITY LIBRARY)
- PRESCOTT/HARLEY/KLEIN'S, MICROBIOLOGY
- MURRAY, ROSENTHAL, PFALLER, MEDICAL MICROBIOLOGY,
- EDWARD K. WAGNER E MARTINEZ J. HEWLETT – BASIC VIROLOGY –BLACKWELL SCIENCE
TEACHERS WILL ALSO PROVIDE TECHNICAL AND EXPERIMENTAL PROTOCOLS FOR THE LABORATORY EXERCISES.

MODULE 1 BACTERIOLOGY
STRUCTURE AND FUNCTION OF THE BACTERIAL CELL. MICROBIAL DIVERSITY. THE DISCOVERY OF MICROBES AND MAIN HISTORICAL EXPERIMENTS. THE CELL WALL OF BACTERIA AND ARCHAEA. THE PEPTIDOGLYCAN: STRUCTURE, FUNCTION, BIOGENESIS. THE STAIN PROPERTIES OF BACTERIA. THE PROKARYOTIC CELL MEMBRANE AND THE MEMBRANE TRANSPORT SYSTEMS. CYTOPLASMIC STRUCTURES (ORGANELLES AND INCLUSIONS) AND THE EXTRA-CYTOPLASMIC STRUCTURES (CAPSULES, PILI, FIMBRIA, FLAGELLA). STRUCTURE AND ORGANIZATION OF THE PROKARYOTIC GENOME. REGULATION OF GENE EXPRESSION IN BACTERIA. THE SPORE STRUCTURE AND REGULATION OF SPORULATION. BACTERIAL CHEMOTAXIS AND OTHER TAXES. NUTRITION, CULTURE AND METABOLISM OF BACTERIA. CHEMOLITOTROPHY. BACTERIAL CELL DIVISION. MICROBIAL GROWTH AND FACTORS AFFECTING GROWTH. CONTROL OF MICROBIAL GROWTH BY CHEMICAL AND PHYSICAL METHODS; STERILIZATION AND DISINFECTION. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. ANTIBIOTIC RESISTANCE. HOST-PARASITE INTERACTIONS AND PATHOGENICITY FACTORS. EXOTOXINS AND ENDOTOXIN. INTRACELLULAR PARASITISM.
MICROBIAL EVOLUTION AND SYSTEMATICS. TAXONOMY AND MAIN TAXONOMIC UNITS IN PROKARYOTES. THE SPECIES CONCEPT IN BACTERIA. THE GENERAL PHYLOGENY OF BACTERIA. METHODS FOR INVESTIGATION OF BACTERIAL PHYLOGENY AND SPECIES IDENTIFICATION. GENOTYPIC ANALYSES. THE ARCHAEA: STRUCTURAL, FUNCTIONAL, METABOLIC AND EVOLUTIONARY CHARACTERISTICS. HYPERTHERMOPHILIC, METHANOGENIC AND HALOPHILIC ARCHAEA. BIODIVERSITY IN PROKARYOTES. OUTLINE OF MICROBIAL BIOTECHNOLOGIES.

BROCK, BIOLOGY OF MICROORGANISMS, EDS: M. T. MADIGAN - J. M. MARTINKO - D. A. STAHL - D. P. CLARK WEB SITES: WWW.MICROBIOLOGYBYTES.COM (CLICK ON SITEMAP AND USE ALSO THE LABWORK: ONLINE EXPERIMENTS ); HTTP://WWW.MICROBEWORLD.ORG/MICROBES/; HTTP://GSBS.UTMB.EDU/MICROBOOK/TOC.HTM; HTTP://WWW.BACT.WISC.EDU/MICROTEXTBOOK/
OTHER RECOMMENDED TEXTBOOKS (AVAILABLE IN THE ROMA TRE UNIVERSITY LIBRARY)
- PRESCOTT/HARLEY/KLEIN'S, MICROBIOLOGY
- MURRAY, ROSENTHAL, PFALLER, MEDICAL MICROBIOLOGY,
- CANN ALAN J.,PRINCIPLES OF MOLECULAR VIROLOGY,
- EDWARD K. WAGNER E MARTINEZ J. HEWLETT – BASIC VIROLOGY –BLACKWELL SCIENCE
TEACHERS WILL ALSO PROVIDE TECHNICAL AND EXPERIMENTAL PROTOCOLS FOR THE LABORATORY EXERCISES.

MODULE 1 BACTERIOLOGY
STRUCTURE AND FUNCTION OF THE BACTERIAL CELL. MICROBIAL DIVERSITY. THE DISCOVERY OF MICROBES AND MAIN HISTORICAL EXPERIMENTS. THE CELL WALL OF BACTERIA AND ARCHAEA. THE PEPTIDOGLYCAN: STRUCTURE, FUNCTION, BIOGENESIS. THE STAIN PROPERTIES OF BACTERIA. THE PROKARYOTIC CELL MEMBRANE AND THE MEMBRANE TRANSPORT SYSTEMS. CYTOPLASMIC STRUCTURES (ORGANELLES AND INCLUSIONS) AND THE EXTRA-CYTOPLASMIC STRUCTURES (CAPSULES, PILI, FIMBRIA, FLAGELLA). STRUCTURE AND ORGANIZATION OF THE PROKARYOTIC GENOME. REGULATION OF GENE EXPRESSION IN BACTERIA. THE SPORE STRUCTURE AND REGULATION OF SPORULATION. BACTERIAL CHEMOTAXIS AND OTHER TAXES. NUTRITION, CULTURE AND METABOLISM OF BACTERIA. CHEMOLITOTROPHY. BACTERIAL CELL DIVISION. MICROBIAL GROWTH AND FACTORS AFFECTING GROWTH. CONTROL OF MICROBIAL GROWTH BY CHEMICAL AND PHYSICAL METHODS; STERILIZATION AND DISINFECTION. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. ANTIBIOTIC RESISTANCE. HOST-PARASITE INTERACTIONS AND PATHOGENICITY FACTORS. EXOTOXINS AND ENDOTOXIN. INTRACELLULAR PARASITISM.
MICROBIAL EVOLUTION AND SYSTEMATICS. TAXONOMY AND MAIN TAXONOMIC UNITS IN PROKARYOTES. THE SPECIES CONCEPT IN BACTERIA. THE GENERAL PHYLOGENY OF BACTERIA. METHODS FOR INVESTIGATION OF BACTERIAL PHYLOGENY AND SPECIES IDENTIFICATION. GENOTYPIC ANALYSES. THE ARCHAEA: STRUCTURAL, FUNCTIONAL, METABOLIC AND EVOLUTIONARY CHARACTERISTICS. HYPERTHERMOPHILIC, METHANOGENIC AND HALOPHILIC ARCHAEA. BIODIVERSITY IN PROKARYOTES. OUTLINE OF MICROBIAL BIOTECHNOLOGIES.

BROCK, BIOLOGY OF MICROORGANISMS, EDS: M. T. MADIGAN - J. M. MARTINKO - D. A. STAHL - D. P. CLARK + CANN ALAN J.,PRINCIPLES OF MOLECULAR VIROLOGY,WEB SITES: WWW.MICROBIOLOGYBYTES.COM (CLICK ON SITEMAP AND USE ALSO THE LABWORK: ONLINE EXPERIMENTS ); HTTP://WWW.MICROBEWORLD.ORG/MICROBES/; HTTP://GSBS.UTMB.EDU/MICROBOOK/TOC.HTM; HTTP://WWW.BACT.WISC.EDU/MICROTEXTBOOK/
OTHER RECOMMENDED TEXTBOOKS (AVAILABLE IN THE ROMA TRE UNIVERSITY LIBRARY)
- PRESCOTT/HARLEY/KLEIN'S, MICROBIOLOGY
- MURRAY, ROSENTHAL, PFALLER, MEDICAL MICROBIOLOGY,
- EDWARD K. WAGNER E MARTINEZ J. HEWLETT – BASIC VIROLOGY –BLACKWELL SCIENCE
TEACHERS WILL ALSO PROVIDE TECHNICAL AND EXPERIMENTAL PROTOCOLS FOR THE LABORATORY EXERCISES.