Università Roma Tre

Basic molecular techniques for the manipulation of nucleic acids. Students will learn how to use pipettes and other common laboratory instruments, and will tackle the various experimental steps aimed at cloning a DNA fragment. In particular, they will explore the use of restriction/modification enzymes, polymerase chain reaction (PCR), and its applications. Finally, they will conduct electrophoretic analysis on agarose gel for the separation of DNA fragments produced by PCR.

REFERENCE TEXTBOOK: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI. FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI.

THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

REFERENCE TEXTBOOK: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI. FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI.

THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

REFERENCE TEXTBOOK: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI. FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI.