LABORATORY OF ELECTRON AND ION BEAM MICROSCOPY APPLIED TO BIOLOGICAL SYSTEMS
(objectives)
THE COURSE AIMS TO PROVIDE THE STUDENT WITH BASIC KNOWLEDGE AND TECHNICAL SKILLS IN ORDER TO APPROACH THE STUDY OF BIOLOGICAL SPECIMENS BY ULTRASTRUCTURAL MORFOLOGICAL METHODOLOGIES. MAIN OBJECTIVES INCLUDE LEARNING AND APPLYING:1) BASIC PRINCIPLES OF ELECTRON MICROSCOPY; 2) METHODOLOGIES OF SAMPLE PREPARATION (PROKARYOTES AND EUKARYOTES) FOR ULTRASTRUCTURAL ANALYSIS; 3) USAGE OF DEVICES FOR ULTRASTRUCTURAL INVESTIGATION (SCNANING, TRANSMISSION AND FOCUSING ION BEAM MICROSCOPY); 3) SOFTWARE FOR MICROSCOPIC IMAGE CAPTURING AND PROCESSING 4) QUALITATIVE INTERPRETATION AND QUANTITATIVE ANALYSIS OF ULTRASTRUCTURAL DATA. THE STUDENT WILL BE ABLE TO AUTONOMOUSLY CHOOSE AND ADJUST EXPERIMENTAL PROTOCOLS, SUITED TO DIFFERENT SAMPLE TYPES AND TO THE OBJECTIVE OF THE STUDY. KNOWLEDGE ACQUIRED DURING THE COURSE WILL ALLOW THE STUDENT TO CRITICALLY ANALYZE MORPHOLOGICAL RESULTS IN A FUNCTIONAL CONTEXT.
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