Università Roma Tre

structure and function of the bacterial cell and microbial diversity
• Discovery of the microbial world: main historical exertions • The cell wall of bacteria and Archea. Peptidoglycan: structure, function, synthesis; biochemical principles of colorations • Cytoplasmic membrane and transport systems in prokaryotes • Cytoplasmic structures (organelles and inclusions) and extra-cytoplasmic (capsule, pili and fimbriae, flagella) • Organization and structure of the genome in prokaryotes • Basic models of regulation gene expression in bacteria • Spore: structure and regulation • Chemotaxis in bacteria • Overview of energy metabolism in prokaryotes • Chemolitotroph bacteria • Cell cycle in bacteria: cell division and growth curve • Control of bacterial growth: sterilization, disinfection , antibiotics and mechanisms of resistance • Host-parasite interactions: pathogenic bacteria and pathogenicity factors • Exotoxins, endotoxin and intracellular parasitism
Overview of taxonomy and bacterial systematic Identification and description of fundamental taxonomic units • The Archea functional and evolutionary structural features. • Archea hyperthermophiles, methanogens and halophiles • Biodiversity of microorganisms. Pure culture isolation methods for typing Analisiphenotypic and antibody use • Genotype analysis DNA analysis (% GC, DNA_DNA reassay,) Use of restriction enzymes • PCR-based analysis. Use of various methods for the typing of microorganisms. • Overview of the use of microorganisms in biotechnology