Optional group:
CARATTERIZZANTI - (show)
 |
12
|
|
|
|
|
|
|
|
|
20410207 -
Biochimica della nutrizione
(objectives)
The Nutrition Biochemistry course is aimed at providing to the students the basic knowledge to understand the molecular mechanisms by which the human organism uses macro- and micro-nutrients to produce the energy needed to maintain the order which characterizes every living being.
In particular, the objectives of the course are the comprehension of the pathways through which the different groups of nutrients (carbohydrates, lipids, proteins, vitamins and minerals) and other substances of natural and non-natural origin (ethanol, stimulant beverages, xenobiotics) are assimilated and transformed in the human body, and how they influence its metabolic status.
Finally, one of the aims is also that of illustrating the production of reactive oxygen species linked to metabolism and the protective role exerted by antioxidant compounds of dietary origin
-
Derived from
20410207 Biochimica della nutrizione in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 DI MASI ALESSANDRA, POLTICELLI FABIO
( syllabus)
Module A - Prof.ssa Alessandra di Masi
- Fat-soluble and water-soluble vitamins
- Macroelements
- Microelements
- Carbohydrates. Classification of carbohydrates. Carbohydrate biosynthesis. Digestion and absorption of carbohydrates. Fate and biological functions of carbohydrates. Regulation of carbohydrate metabolism.
- Lipids. Classification of lipids. Lipid digestion and absorption. Fate and biological functions of lipids. Regulation of carbohydrate metabolism.
- Proteins. Daily protein requirement. Nutritional properties of proteins. Protein digestion and amino acid fate. Biosynthesis of non-protein nitrogen compounds.
( reference books)
- Leuzzi, Bellocco, Barreca “Biochimica della Nutrizione” Ed. Zanichelli
- Cozzani, Dainese "Biochimica degli alimenti e della nutrizione" Ed. Piccin.
- Biochemistry texts used for the preparation of the Biochemistry exam of the three-year degree course in Biological Sciences.
RECEPTION TIME: Monday 10 am
|
6
|
BIO/10
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410253 -
NEUROBIOLOGIA CELLULARE APPLICATA
(objectives)
The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. in this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
|
6
|
BIO/06
|
32
|
-
|
20
|
-
|
Core compulsory activities
|
ITA |
|
20410486 -
Laboratorio di neurobiologia cellulare
(objectives)
The aims of this course are:
1)to gain knowledge of the specialized cellular architecture of glia and neurons;
2)to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons.
Special emphasis will be given to nitric oxide-regulated pathways.
In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs.
Moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
-
Derived from
20410486 Laboratorio di neurobiologia cellulare in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 PERSICHINI TIZIANA
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology.
Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity.
Topics:
The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA”
– THIRD EDITION – 2012 OXFORD UNIVERSITY PRESS
Slides in PDF format of all the lectures will be provided by the teacher.
The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it
|
6
|
BIO/06
|
32
|
20
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20402369 -
MICROBIAL GENETICS
(objectives)
The main educational goal of the genetics of microorganisms course is to provide students with knowledge on:
1) the prokaryotic genome and the mechanisms that ensure its replication, transcription, translation and integrity throughout generations;
2) the regulation mechanisms of the genetic expression in bacteria;
3) the mechanisms responsible for horizontal gene transfer (conjugation, transformation and transduction);
4) the molecular basis of cellular differentiation and adaptive response.
Students will also acquire basic knowledge on genetic engineering and omic approaches to study microbial communities.
-
Derived from
20401664 GENETICA DEI MICRORGANISMI in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 N0 IMPERI FRANCESCO
( syllabus)
The course aims at providing to the student:
- adequate knowledge and understanding on advanced methodologies in bacterial genetics, appropriate methodologies and communication skills for the study of genotype-phenotype relationships in bacteria;
- adequate applied knowledge for the evaluation and drafting of experimental approaches, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).
Programme
- structure, organization, functioning and evolution of the bacterial chromosome;
- methods for the sequencing and analysis of bacterial genomes;
- structure, organization, function and evolution of mobile genetic elements (plasmids and transposons);
- genetic and molecular bases of horizontal gene transfer;
- genetic and molecular bases of gene expression regulation and bistability in bacteria;
- genetic bases and experimental approaches for the study of antibiotic resistance and tolerance;
- methods for the identification and validation of essential genes;
- approaches for the identification and study of unculturable microorganisms
( reference books)
Selected topics will be available on suggested Bacterial Genetics books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices.
Innovative teaching will be promoted by sharing power-point presentations illustrating the main topics of the course via email. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.
|
6
|
BIO/18
|
40
|
10
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410314 -
GENETICA DEI SISTEMI COMPLESSI
(objectives)
The course aim to provide a wider and deeper outlook of genetics topics not addressed in the course of genetics.
Special attention will be paied to the role of human genome (and epigenome) in the regulation of complex biological processes and in the individual response to endogenous and exogenous factors.
|
6
|
BIO/18
|
40
|
-
|
10
|
-
|
Core compulsory activities
|
ITA |
|
20401821 -
IMMUNOLOGY
(objectives)
Cellular and molecular mechanisms involved in the immune response with a particular interest for those implicated in the response against pathogens.
-
Derived from
20401821 IMMUNOLOGIA in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 N0 Coccia Eliana Marina
( syllabus)
Properties and Overview of Immune Responses: Cells and Tissues of the Immune System
Leukocyte Circulation and Migration into Tissues
Innate Immunity
Antigens and Antigen presentation
Immune Receptors and Signal Transduction
Lymphocyte T Development and Activation
Differentiation and Functions of CD4+ and CD8 +Effector T Cells
B Cell Activation and Antibody Production
Humoral Immunity
Immunologic Tolerance and Autoimmunity
Immunity to Microbes and Vaccines
Immunodeficiency and immunosenescence
Immunological techniques
SEMINAR:
PAMPS AND TLRS
DENDRITIC CELLS
MICRO-RNA
IMMUNOPHAGY
BIBLIOGRAPHIC RESARCH - PUBMED
( reference books)
- Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai
IMMUNOLOGIA CELLULARE E MOLECOLARE
(8th Edition, Edizioni Edra)
- Peter Parham
IL SISTEMA IMMUNITARIO
(2nd Edition, Edizioni EdiSES)
|
6
|
MED/04
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410273 -
NEUROBIOLOGIA DELLO SVILUPPO
(objectives)
Aim of the course is to provide students with advanced knowledge of developmental biology, focussing on vertebrate nervous system.
Cellular and molecular mechanisms underlying neural differentiation and morphogenesis will be addressed in different in vivo and in vitro models.
The role of neurogenesis and plasticity throughout the lifespan of individuals will be examined.
Students will be able to:
1) describe major neural processes in vertebrates;
2) identify molecular pathways driving stem cells to neuronal or glial cell fates;
3) correlate abnormal cellular processes with specific neurodevelopmental or age-related disorders.
-
Derived from
20410273 NEUROBIOLOGIA DELLO SVILUPPO in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 MORENO SANDRA
( syllabus)
Overview of developmental processes of pluricellular organisms. Germ cell layers. Ectodermal derivation of neural tissue in invertebrates e vertebrates. Specification of neuroectoderm and neurulation process in vertebrates. Primary neurulation: cellular e molecular mechanisms of neural plate, folds, furrow, and tube formation. Secondary neurulation. Neural tube defects in mammals: genetic and environmental causes of anencephaly and spina bifida. Morphogenesis and differentiation of the encephalon and spinal cord. Role of cell death in the morphogenesis of the central nervous system: apoptotic and autophagic pathways. Neural crest: specification and regionalization. Cell migration and differentiation of cephalic, cardiac, trunk, vagal e sacral neural crest. Multipotency e plasticity of neural crest cells. Layering of cortical structures in the cerebrum and cerebellum. Congenital diseseases associated with defects to neuronal migration: the case of peroxisomal disorders. Neural stem cells: neuronal and glial differentiation. Adult neurogenesis in mammals: the subventricular and subgranular zones. The concept of stem cell niche. Regulatory pathways of the neurogenic niche. Neurogenesis and neurodegeneration in the senescent brain. Age-related neurodegenerative diseases: common and specific features. Protein aggregates, role of apoptotic and autophagic pathways, and stem cell-based therapies against neurodegeneration.
( reference books)
Gilbert: "Developmental Biology", Tenth edition, Sinauer Associates, Inc., 2014
Sanes et al.: "Development of the nervous system", Elsevier Inc., 2012
|
6
|
BIO/06
|
40
|
-
|
10
|
-
|
Core compulsory activities
|
ITA |
|
20410212 -
Genetica umana applicata
(objectives)
The main aims of the course are:
1. To consolidate knowledge in Human Genetics acquired during previous basic courses, with particular reference to biological mechanisms involved in genetic diseases
2. To carry on theorical notions on a practical point of view by the knoledge of diagnostic laboratory methods and strategies
|
6
|
BIO/18
|
24
|
-
|
30
|
-
|
Core compulsory activities
|
ITA |
|
20410278 -
MICROBIOLOGIA AMBIENTALE
(objectives)
The main aims of the course are:
1. acquire knowledge of the fundamental role of microorganisms in ecosystems and of the factors affecting their distribution and interactions with other organisms:
- metabolic and functional biodiversity, structure and dynamics of microbial communities
- bacteria and archea taxonomic groups
2. knowledge of traditional, molecular and cultivation-independent methods for identification/typing and analysis of microbial populations
3. evaluation of the multiple potential applications of environmental microorganisms also as bioindicators
4. acquisition of critical skills by reading scientific articles.
-
Derived from
20410278 MICROBIOLOGIA AMBIENTALE in Biodiversità e gestione degli ecosistemi LM-6 DALMASTRI Claudia
( syllabus)
1. Evolution and systematic of microorganisms
Origin of bacteria; molecular phylogenesis; 16S rRNA gene and evolution; basis of microbial systematic
2. Metabolic diversity of microbes
Chemolitotrophy; fermentation; anaerobiosis; phototrophy
3. Functional diversity of microbes
Phototrophic and chemotrophic bacteria; bacteria in sulfur and nitrogen cycles
4. The principal phylogenesic groups of Bacteria
Proteobacteria, Firmicutes, Bacteroidetes, Ipertermofili
5. The principal phylogenetic groups of Archea
Phyla and evolution; Euryarchaeota;extremophiles
6. Methods in microbial ecology
Culture-based methods; functional analysis
Cultivation-independent techniques: microscope; genetic analysis; genome and metagenomic
7. Microbial ecosystems
Principles of ecology
Microbe environment: microbial interactions (Quorum sensing; Biofilm)
Terrestrial environment: soil
Aquatic environment: lakes, oceans
Extreme environments (deep sea; hydrothermal springs)
8. Microbial role in nutrient cycles
Carbon, nitrogen, sulfur, others
9. Symbiosis between microorganisms and between microorganisms and different organisms
Plants; rhizosphere; root nodules
Mammals; microbiome: bowel and rumen
10. Microorganisms in anthropized environments
Bioremediation of contaminated sites; water treatment; biocorrosion; mineral recovery from mines
( reference books)
Text book: Madigan et al., Brock Biology of microorganisms 14th edition
|
6
|
BIO/19
|
44
|
-
|
5
|
-
|
Core compulsory activities
|
ITA |
|
20410213 -
Nutrienti di origine vegetale
(objectives)
The main objective of the course is to provide basic knowledge on the main nutrients present in different food plants, such as carbohydrates, lipids and proteins. Particular attention is paid to the biochemical plasticity of the secondary metabolism which leads to the formation of both secondary metabolites and bioactive compounds with beneficial effects on human health, and of anti-nutritional or toxic factors.
-
Derived from
20410213 Nutrienti di origine vegetale in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 CONA ALESSANDRA
( syllabus)
Origin of agriculture and plant domestication. Characteristics of the main crops: Poaceae, Fabaceae, Rosaceae, Solanaceae, Brassicaceae. Nutritional values of the different plant organs and nutrients bioavailability. Primary and secondary metabolism. Nutrients from plants: proteins, lipids and oils, carbohydrates, fibers, minerals, vitamins and antioxidants. Nutritional values of secondary metabolism and bioactive compounds: terpenes, phenols, alkaloids, cyanogenic glucosides, glucosinolates, non-protein amino acids. Effects of the environmental conditions on primary and secondary metabolism: food quality and production of aromas, flavors and toxins. Plant bioactive compounds as dietary supplements. Effects of storage conditions and pre- and post-harvest diseases on nutritional quality of plant foods. Allergy and intolerances caused by plant substances present in crop species. Genetic improvement and biotechnology approaches to develop crop plants with high nutritive contents and resistant to environmental stress.
( reference books)
1. Piante alimentari. Biologia, composizione chimica, utilizzazione. Rinallo C.; Piccin.
2. Biologia delle Piante. Vol 2: Interazioni con l’ambiente e Domesticazione. Smith A. M. et al.; Zanichelli.
3. Lecture notes and scientific articles provided by the teacher
The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it
|
6
|
BIO/04
|
44
|
5
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410214 -
Nutrigenomica
(objectives)
The course will deal with diet-genome interactions and the application of nutritional strategies in health maintenance.
Students should become familiar with the concept that external molecules present in our foods can affect human metabolism and gene expression in different cells and tissues. The course will address the utilization of the concept of “system biology” to nutrition
|
6
|
BIO/11
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20402377 -
GENERAL PATHOLOGY
(objectives)
The aims of the course are:
•to know the aetiology and the molecular mechanisms of the human diseases;
•to know how to interpret the pathophysiological mechanisms of the human diseases
|
6
|
MED/04
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410319 -
FISIOLOGIA CELLULARE E MOLECOLARE
(objectives)
The course aims to deliver knowledge on some aspects of the regulation of cellular function on the basis of the most recent published works.
|
6
|
BIO/09
|
40
|
-
|
10
|
-
|
Core compulsory activities
|
ITA |
|
20410491 -
Fisiologia della risposta cellulare allo stress
(objectives)
The primary objective of the course is to enlarge and update student’s knowledge about the physiology and regulation of cellular functions mainly regarding the molecular mechanisms activated by cells in response to stressing conditions to maintain intracellular homeostasis.
-
Derived from
20410491 Fisiologia della risposta cellulare allo stress in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 FIOCCHETTI MARCO
( syllabus)
Introduction to the molecular mechanisms of cellular functions regulation: membrane and nuclear receptors, mechanisms of intracellular signal transduction and amplification through protein’s post-translational modifications (e.g. phosphorylation).
Definition of cellular stress and physiological tolerance range. Description of intracellular mechanisms, at DNA, RNA and protein levels, through which cells sense and respond to a broad variety of stressing conditions, focusing the attention mainly on physiological response and cell adaptation to extracellular stressing stimuli, including heat, osmotic, hypoxic, oxidative and nutrient stresses.
Evaluation of intracellular organelles- specific stress response, including mitochondria- and ER-stress response.
Description of down-stream molecular mechanisms activated by cells in response to stress and devote to re-establish the intracellular homeostasis and to adapt themselves to the changed conditions. Brief description of the abnormal stress response and adaptation in pathological cell degeneration (e.g. cancer).
Definition and update of the state-of-art about the laboratory research approaches applied to the study of the physiological stress response and adaptation and the molecular mechanisms behind these functions.
( reference books)
The class will be provided with the study materials found by students and by the professor.
|
6
|
BIO/09
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410495 -
Microbiomica
(objectives)
The goal of the Microbiomics course is to provide information about the structure, dynamics, evolution, and methods to investigate complex microbial communities as a whole. The rapid progress of omic sciences (genomics, transcriptomics, proteomics and metabolomics) combined with high-throughput genome sequencing, global gene expression analysis tools, and powerful analytical method of metabolites has made feasible the analysis of complex and diverse microbial communities and their products as a whole.
The course is intended to provide state-of-art information on the impact of the microbiota in agriculture, food production, as well as animal and human health
-
Derived from
20410495 Microbiomica in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 VISCA PAOLO
( syllabus)
BASIC TOPICS: Introduction to the genomics of prokaryotes and its study techniques (sequencing, annotation, gene family content, duplications and deletions); Horizontal gene transfer and mobilome; metagenomics; Transcriptomics and gene chips; Proteome and interactome; metabolomics; Genomics of single cells; Systems biology and health; Genetic manipulations - Expression of heterologous genes in bacteria - Evolution of bacterial genomes; Molecular phylogeny; Microbial taxonomy methods and species concept; Classification of prokaryotes; Culture analysis of microbial communities; Enrichment cultures; Isolation of single cells; Laser forceps; Flow cytometry; Microfluidic techniques; High-throughput systems; Staining methods; Fluorescent in-situ hybridization (FISH); Gene amplification methods for the analysis of microbial communities; Microarray analysis of the genetic and functional diversity of microorganisms; Environmental genomics and study methods; Measurement of microbial activity in nature; Examples of symbiosis: lichens, intestinal polymicrobial systems (rumen); General information on the huiman microbiome; Gastrointestinal microbiota; Oral and airway microbiota; Microbiota of the urogenital tract and skin; Development and evolution of the human microbiota; Studies on humans and in animal models; Colonization, microbial sequences and stability of the intestinal microbiota; Diseases associated with intestinal dysbiosis; Diseases associated with dysbiosis of the oral cavity, skin and genital tract; Antibiotics, resistome and induced alterations of the microbiome; Probiotics and prebiotics; Microbiota and intestine-brain axis.
APPLICATIONS: Technologies for sequencing and NGS; Culturome and chemotaxonomy; Sequencing data preparation and analysis; Phylogeny based on 16S rRNA, 25-28S rRNA, ITS etc .; Tools to analyze the structure and dynamics of the microbiota: Whole Genome Sequencing (WGS) and metagenomics shotgun; Analytical databases, tools and pipelines (annotations); Resistome; Virulome; Virome.
IN-DEPTH: illustration of monothematic articles on the microbiota of humans, animals and plants, in relation to physio-pathological alterations. Groundbreaking articles and reviews will be selected from scientific journals year by year.
( reference books)
"Brock biology of microorganisms" (English) by Michael T. Madigan (Author), Kelly S. Bender (Author), Daniel H. Buckley (Author), W. Matthew Sattley (Author), David A. Stahl (Author): chapters 9.1-9.14; 12.1-12.5; 12.9; 12.11-12.13; 13.5-13.10; 19.1-19.12; 23.1; 23.12, 23.13; 24.1-24.11
Microbiota in health and disease: from pregnancy to childhood. Editors Pamela D. Browne, Eric Claassen and Michael D. Cabana Published: 2017 Pages: 344. eISBN: 978-90-8686-839-1 | ISBN: 978-90-8686-294-8 https://doi.org/10.3920/978-90-8686-839-1
The human microbiota: how microbial communities affect health and disease / edited by David N. Fredricks.
ISBN 978-0-470-47989-6
|
6
|
BIO/19
|
48
|
-
|
-
|
-
|
Core compulsory activities
|
ITA |
|
20410321 -
VIROLOGIA ANIMALE GENERALE ED APPLICATA
(objectives)
Deepening the knowledge about animal viruses (origins, classification, replicative strategies, virulence, immunoevasion mechanisms) and their applications (antiviral vaccines, viral vectors for gene expression, oncolytic viruses).
-
Derived from
20410321 VIROLOGIA ANIMALE GENERALE ED APPLICATA in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 AFFABRIS ELISABETTA
( syllabus)
The course describes and compares the replication cycles of different animal viruses that infect vertebrate hosts to gain insight into: origin of viruses, structure, evolution, virus-cell interactions, host defense mechanisms and strategies of viral immunoevasion, methods of detection, identification and titration, antiviral drugs and profilaxis of viral infections. In particular, it will be described viruses of the following viral families: Parvoviridae, Poliomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae, Picornaviridae, Flaviviridae, Coronaviridae, Rabdoviridae, Orthomixoviridae, Paramixoviridae, Retroviridae, Hepadnaviridae. In addition, introduction to applied virology will be provided (i.e., vaccine development, viral vectors and oncolytic viruses). Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
All the detailed list of topics covered during the lessons, the PDF files of power point presentations used during the course and further materials are available in the Moodle platform of the course.
Adopted text book:
- N. J. Dimmock, A. J. Easton, K. N. Leppard - Introduction to Modern Virology - Seventh edition 2016 -Wiley Blackwell - ISBN 978-1-119-97810-7
- Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press (this text book is more detailed)
to complement the PDF files of the power point used by the teacher during the course.
Mandatory integrations of the PDF files regarding viral vectors (adenoviral, adeno-associated, retroviral and lentiviral vectors, poxvirus-based vectors) and oncolitic viruses (general introduction + oncolytic adenoviruses) can be found herebelow:
- Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (on line access through Roma Tre Scientific Library)
- Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159.
- Carina Elsner and Jens Bohne - The retroviral vector family: something for everyone. – Virus Genes 2017, 53:714-722
The teacher receives by appointment via email (elisabetta.affabris@uniroma3.it) or at the end of the lessons.
|
6
|
BIO/19
|
40
|
-
|
10
|
-
|
Core compulsory activities
|
ITA |
|
|
Optional group:
AFFINI E INTEGR - (show)
|
12
|
|
|
|
|
|
|
|
|
20410207 -
Biochimica della nutrizione
(objectives)
The Nutrition Biochemistry course is aimed at providing to the students the basic knowledge to understand the molecular mechanisms by which the human organism uses macro- and micro-nutrients to produce the energy needed to maintain the order which characterizes every living being.
In particular, the objectives of the course are the comprehension of the pathways through which the different groups of nutrients (carbohydrates, lipids, proteins, vitamins and minerals) and other substances of natural and non-natural origin (ethanol, stimulant beverages, xenobiotics) are assimilated and transformed in the human body, and how they influence its metabolic status.
Finally, one of the aims is also that of illustrating the production of reactive oxygen species linked to metabolism and the protective role exerted by antioxidant compounds of dietary origin
-
DI MASI ALESSANDRA
( syllabus)
Module A - Prof.ssa Alessandra di Masi
- Fat-soluble and water-soluble vitamins
- Macroelements
- Microelements
- Carbohydrates. Classification of carbohydrates. Carbohydrate biosynthesis. Digestion and absorption of carbohydrates. Fate and biological functions of carbohydrates. Regulation of carbohydrate metabolism.
- Lipids. Classification of lipids. Lipid digestion and absorption. Fate and biological functions of lipids. Regulation of carbohydrate metabolism.
- Proteins. Daily protein requirement. Nutritional properties of proteins. Protein digestion and amino acid fate. Biosynthesis of non-protein nitrogen compounds.
( reference books)
- Leuzzi, Bellocco, Barreca “Biochimica della Nutrizione” Ed. Zanichelli
- Cozzani, Dainese "Biochimica degli alimenti e della nutrizione" Ed. Piccin.
- Biochemistry texts used for the preparation of the Biochemistry exam of the three-year degree course in Biological Sciences.
RECEPTION TIME: Monday 10 am
-
POLTICELLI FABIO
( syllabus)
- Metabolism of the main alcoholic and psychoactive drinks of nutritional value
- Signal transduction and regulation of metabolic pathways
- Metabolic regulation of the nutrition-fasting cycle
- Reactive oxygen species
- Endogenous and exogenous antioxidants
- Xenobiotics
( reference books)
- Leuzzi, Bellocco, Barreca “Biochimica della Nutrizione” Ed. Zanichelli
- Cozzani, Dainese "Biochimica degli alimenti e della nutrizione" Ed. Piccin.
- General Biochemistry textbooks adopted for the Biochemistry Course of the bachelor degree in Biological Sciences.
|
6
|
BIO/10
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410212 -
Genetica umana applicata
(objectives)
The main aims of the course are:
1. To consolidate knowledge in Human Genetics acquired during previous basic courses, with particular reference to biological mechanisms involved in genetic diseases
2. To carry on theorical notions on a practical point of view by the knoledge of diagnostic laboratory methods and strategies
|
6
|
BIO/18
|
24
|
-
|
30
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410314 -
GENETICA DEI SISTEMI COMPLESSI
(objectives)
The course aim to provide a wider and deeper outlook of genetics topics not addressed in the course of genetics.
Special attention will be paied to the role of human genome (and epigenome) in the regulation of complex biological processes and in the individual response to endogenous and exogenous factors.
|
6
|
BIO/18
|
40
|
-
|
10
|
-
|
Related or supplementary learning activities
|
ITA |
|
20401653 -
BIOCHEMISTRY AND MOLECULAR CLINICAL BIOLOGY
(objectives)
Main goals of the course are:
i) to provide an overview of the activities of a clinical laboratory looking at the organizational context and current business;
ii) to illustrate the various stages which are at the basis of main analytical methods, either molecular or biochemical, used in biomedical laboratories for modern diagnostic purposes;
iii) to provide the key of interpretation for the obtained results from a pathophysiological point of view.
To promote a critical approach towards laboratory diagnosis.
-
BARONI SILVIA
( syllabus)
• LABORATORY MEDICINE AND CLINICAL BIOCHEMISTRY
BIOCHEMICAL TESTS AND LABORATORY MANAGEMENT
PREANALYTICAL, ANALYTICAL AND BIOLOGICAL VARIABILITY
REFERENCE VALUES
• QUALITY CONTROL ASSURANCE
• ANALYTICAL METHODS
IMMUNOCHEMISTRY AND INTERFERENCES
• LABORATORY ANDCARDIOVASCULAR DISEASES
LIPOPROTEIN ALTERATIONS
LABORATORY AND DIABETES MELLITUS
MARKERS OF CARDIAC DISEASES
• LABORATORY AND RENAL FUNCTION
URINALYSIS
• LABORATORY AND LIVER
• LABORATORY AND NEUROLOGICAL DISEASES
CEREBROSPINAL FLUID
• LABORATORY AND ONCOLOGY
• LABORATORY AND MALE INFERTILITY
• INTRODUCING CLINICAL MOLECULAR BIOLOGY
( reference books)
Clinical Biochemistry: An Illustrated Colour Text- Allan Gaw MD, ICT, 2013
|
6
|
BIO/12
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410208 -
Biologia marina
(objectives)
The course is one of the optional training activities of the Master’s Degree in “Biodiversity and management of Ecosystems” and allows students to acquire a basic knowledge of Marine Biology and of the marine environment conservation, with a specific attention given to the Mediterranean Sea.
Educational objectives of the course are:
1) to acquire basic skills of Oceanography, related to the physico-chemical factors and the movement of the sea;
2) to acquire basic skills related to the biology and adaptations of marine organisms, as well as the most important Mediterranean species and habitats of conservation interest and Mediterranean biogeography;
3) to improve knowledge about monitoring and data collection techniques in the marine environment;
4) to acquire the concepts and skills related to the critical issues existing in the Mediterranean Sea, and the tools availablefor its management and protection.
|
6
|
BIO/07
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20401676 -
FRESH WATER ECOLOGY
|
|
|
-
I modulo
|
3
|
BIO/07
|
20
|
5
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
-
II modulo
|
3
|
BIO/07
|
20
|
5
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20401656 -
BIOGEOGRAPHY
(objectives)
After the course, the students should be able to:
1) discuss the historical and ecological factors affecting the geographical distribution of animals, plants and other organisms;
2) formulate hypotheses and models explaining the shape, size and position of the geographic range of a taxa;
3) give an interpretation of recent events of range expansion or contraction, due to climatic changes or habitat transformation caused by human activities;
4) describe the fauna, flora and vegetation of each continent, in relation to past and present, geographical and ecological factors;
5) give a contribution to biodiversity conservation and management by identifying the most possible number of hotspots in all biogeographical regions, by monitoring invasive alien species and providing data for the development of predictive models that take into account the climate changes and landscape alteration.
-
Derived from
20401656 BIOGEOGRAFIA in Biodiversità e gestione degli ecosistemi LM-6 N0 BOLOGNA MARCO ALBERTO
( syllabus)
(a) Introductive elements.
What is Biogeography: This Science which considers synthetically information from Geography, Palaeogeography, Geology, Ecology, Palaeoecology, Phylogenetic Systematics, Faunistic, Floristic, Population Genetics, Evolutionary Biology. Summary of principles of Evolutionary Biology (micro and macro-evolution), Systematics (Phenetist, Evolutionary and Cladistic schools), ecosystem and population Ecology. Speciation models, radiation. Natural and anthropic causes of extinction. Biogeography and Conservation of Nature.
Story of Biogeography: The founders of the Science: Buffon, de Candolle, von Humboldt, Lyell, Hooker, Sclater, Darwin, Wallace, Haeckel, Merriam, Simpson, Darlington, Holdaus, Gridelli, De Lattin, Furon, La Greca, Croizat, Wilson e MacArthur, Rozen e Platnick, Morrone, Avise, Hewitt.
Biogeographic schools of the XX century: Historical Biogeography, bridges, filters, dispersal and dispersion; Croizat and Panbiogeography; Cladistic Biogeography; Ecological Biogeography; Statistic Biogeography; Molecular Biogeography and Phylogeography. The Italian School of Biogeography: Gridelli, La Greca, Baccetti, Ruffo, Vigna Taglianti, Poldini; the Italian Society of Biogeography.
(b) Historical Biogeography
Land and marine Biogeography: History of life on the Earth: Tectonic of terrestrial plates and the Continental drift Theory; macro-plates and micro-plates; evolution and displacement of terrestrial masses and seas; evolution of terrestrial ecosystems. Climatic and biogeographic effects of plates tectonic. Effects of Pliocene-Pleistocene glaciations; Pleistocene glacial refugia; megafauna extinction; expansion and contraction of biomes. Effects of glaciations on lands and seas in temperate and tropical regions.
The terrestrial biogeographic regions: Floristic realms and Zoogeographic Regions. Causes of floras and faunas differentiation of Biogeographic regions. Regions and sub-regions. Marine Biogeographic Regions. Palaearctic Region (boundaries; sub-regions; distinctive elements). Nearctic Region (boundaries; sub-regions; distinctive elements). Oriental (Indo-Malayan) Region (boundaries; sub-regions; distinctive elements). Afrotropical Region (boundaries; sub-regions; distinctive elements). Neotropical Region (boundaries; sub-regions; distinctive elements). Australian-Oceanic (Australasian) Region (boundaries; sub-regions; distinctive elements)). Antarctic (boundaries; sub-regions; distinctive elements). Transitional Biogeographic Regions: Saharo-Sindian; Chinese; Indo-Australian (Wallacea); Meso-American. Regionalization of floras and faunas.
Biomes and Biogeographic Regions: Effects of climate and climatic cycles. Characteristics and geological origin in the Biogeographic regions. Effects of water circulation and of the sea depth on the marine regions. Biomes, and their distribution on the lands; dynamics of biomes. Marine biomes. Differences between biomes and biogeographic regions. Biomes and ecosystems. Anthropic transformation of biomes.
Biogeography of the Mediterranean area and the Europe: Tethys and Paratethys and Mediterranean origin. Shift, migration and positioning of microplates. Biogeography of Cenozoic: Messianian salinity crisis; Pliocene-Pleistocene effects of glaciations and refugia.
Range: specific and over-specific ranges; continuous, fragmented, disjunct ranges; historical and ecological causes of ranges; shape of ranges; terrestrial and marine ranges. Primary and Secondary ranges. Physical, ecological and palaeogeographic factors affecting limits of ranges: present barriers and limiting factors. Relicts. Endemism: ranges and conservation.
Generalized distributional models (chorotypes): examples in the Palaearctic and Afrotropical Regions. Examples of distribution of land plants and animals.
Dispersalist Biogeography (Simpson, Mayr). Dispersal and dispersion. Dispersion as biogeographic (range extension), evolutionary (genetic flow and speciation) and ecological process (niche realization). Overall similarity principles. Species-specific process and generalized models. Active and passive dispersal in plants and animals. Types of dispersal: jump dispersal, stepping stones dispersal. Dispersal and range enlargement. Barriers to dispersal (geographic and ecological). Colonization. Effects on biota due to immigrations.
Vicariance Biogeography. Vicariance (allopatric model; collapse of barrier model). Effects on biota. Cladistic Systematics and Vicariance Biogeography. Croizat’, Morrone’, Nelson’ and Platnick’ Vicariance models.
Distribution and abundance of populations. Variation of distribution and time. Range dynamic and conservation. Fossil and present ranges. Local and complete extinction.
Geographic variation in species (morphological and genetic characteristics). Continuous and discrete variation. Evolutionary and biogeographic importance of variation. Geographic variation and conservation. Geography of divergence and regionalization.
(c) Ecological Biogeography
Ecological Biogeography: present causes of species distribution; realized niche; colonization and competition. Distribution and dynamics of communities, ecosystems and biomes.
Island Biogeography: Wilson and Mac Arthur theory; experimental confirmation and problematic examples; Island Biogeography and Conservation Biology. Examples on geographic and ecological islands and on mountain peaks.
(d) New methods of biogeographic analysis
Molecular Biogeography. Phylogeography (mtDNA, nDNA, molecular and statistical methods). Examples on glacial refugia and postglacial spread.
Statistical Biogeography. Analysis of molecular data in Biogeography; dating; direction of dispersal; time of vicariance.
Cartographic representation of the biogeographic diversity: traditional cartography, areograms, georeferenced databases, GIS and remote-sensing systems. Mapping the ranges.
( reference books)
Lomolino M.V., Riddle B.R. & Whittaker R.J., Biogeography. Biological Diversity across Space and Time. Sinauer, Sunderland, USA.
Also useful: Zunino M. & Zullini A., Biogeografia. La dimensione spaziale dell’evoluzione. Ambrosiana, Milano.
The teacher supplies students with specialist literature and additional material.
|
6
|
BIO/05
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20401664 -
MICROBIAL GENETICS
(objectives)
The main educational goal of the genetics of microorganisms course is to provide students with knowledge on:
1) the prokaryotic genome and the mechanisms that ensure its replication, transcription, translation and integrity throughout generations;
2) the regulation mechanisms of the genetic expression in bacteria;
3) the mechanisms responsible for horizontal gene transfer (conjugation, transformation and transduction);
4) the molecular basis of cellular differentiation and adaptive response.
Students will also acquire basic knowledge on genetic engineering and omic approaches to study microbial communities.
-
IMPERI FRANCESCO
( syllabus)
The course aims at providing to the student:
- adequate knowledge and understanding of advanced methodologies in bacterial genetics, appropriate methodologies and communication skills for the study of genotype-phenotype relationships in bacteria;
- adequate applied knowledge for the evaluation and drafting of experimental approaches, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgments).
Program
- structure, organization, functioning and evolution of the bacterial chromosome;
- methods for the sequencing and analysis of bacterial genomes;
- structure, organization, function and evolution of mobile genetic elements (plasmids and transposons);
- genetic and molecular bases of horizontal gene transfer;
- genetic and molecular bases of gene expression regulation and bistability in bacteria;
- genetic bases and experimental approaches for the study of antibiotic resistance and tolerance;
- methods for the identification and validation of essential genes;
- approaches for the identification and study of unculturable microorganisms
( reference books)
Selected topics will be available on suggested Bacterial Genetics books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices.
Innovative teaching will be promoted by sharing power-point presentations illustrating the main topics of the course via email. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.
|
6
|
BIO/18
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410278 -
MICROBIOLOGIA AMBIENTALE
(objectives)
The main aims of the course are:
1. acquire knowledge of the fundamental role of microorganisms in ecosystems and of the factors affecting their distribution and interactions with other organisms:
- metabolic and functional biodiversity, structure and dynamics of microbial communities
- bacteria and archea taxonomic groups
2. knowledge of traditional, molecular and cultivation-independent methods for identification/typing and analysis of microbial populations
3. evaluation of the multiple potential applications of environmental microorganisms also as bioindicators
4. acquisition of critical skills by reading scientific articles.
-
Derived from
20410278 MICROBIOLOGIA AMBIENTALE in Biodiversità e gestione degli ecosistemi LM-6 DALMASTRI Claudia
( syllabus)
1. Evolution and systematic of microorganisms
Origin of bacteria; molecular phylogenesis; 16S rRNA gene and evolution; basis of microbial systematic
2. Metabolic diversity of microbes
Chemolitotrophy; fermentation; anaerobiosis; phototrophy
3. Functional diversity of microbes
Phototrophic and chemotrophic bacteria; bacteria in sulfur and nitrogen cycles
4. The principal phylogenesic groups of Bacteria
Proteobacteria, Firmicutes, Bacteroidetes, Ipertermofili
5. The principal phylogenetic groups of Archea
Phyla and evolution; Euryarchaeota;extremophiles
6. Methods in microbial ecology
Culture-based methods; functional analysis
Cultivation-independent techniques: microscope; genetic analysis; genome and metagenomic
7. Microbial ecosystems
Principles of ecology
Microbe environment: microbial interactions (Quorum sensing; Biofilm)
Terrestrial environment: soil
Aquatic environment: lakes, oceans
Extreme environments (deep sea; hydrothermal springs)
8. Microbial role in nutrient cycles
Carbon, nitrogen, sulfur, others
9. Symbiosis between microorganisms and between microorganisms and different organisms
Plants; rhizosphere; root nodules
Mammals; microbiome: bowel and rumen
10. Microorganisms in anthropized environments
Bioremediation of contaminated sites; water treatment; biocorrosion; mineral recovery from mines
( reference books)
Text book: Madigan et al., Brock Biology of microorganisms 14th edition
|
6
|
BIO/19
|
44
|
-
|
5
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410486 -
Laboratorio di neurobiologia cellulare
(objectives)
The aims of this course are:
1)to gain knowledge of the specialized cellular architecture of glia and neurons;
2)to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons.
Special emphasis will be given to nitric oxide-regulated pathways.
In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs.
Moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
-
PERSICHINI TIZIANA
( syllabus)
Cultural knowledge: The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. In this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. Moreover students are allowed to critically discuss the scientific literature in the field of glial cell biology.
Methodological skills: to know how to design an experiment using glial cell cultures, to acquire and analyze the data obtained from the laboratory activity.
Topics:
The course will deepen the following topics: morphology and ultrastructure of glial cells; Properties and functions of astrocytes; homeostasis of pH, K and volume; The neuro-glial-vascular unit, mechanism of cerebral blood flow regulation; gliotransmitters release and the tripartite synapse; The role of nitric oxide as neurotransmitter and neurotoxic factor; Microglia: activation, motility and immune surveillance; Reactive astrocytosis, neurodegenerative and infectious diseases. Cellular response to oxidative stress. The laboratory activity will be mainly devoted to the study of nitric oxide as cellular messenger in glial cell cultures. Experimental procedures include but are not limited to the following: Preparation of whole, cytoplasmic and nuclear extracts, Western Blot; Analysis of gene expression and transcription factor activation (RNA extraction, RT-PCR, EMSA, TransAM-ELISA); Modulation of gene expression (Oligo-Decoy); Cell Transfection; Analysis of nitric oxide (NO) pathway and determination of NO in living cells and in cell extracts.
( reference books)
BRUCE R. RANSOM, HELMUT KETTENMANN. “NEUROGLIA”
– THIRD EDITION – 2012 OXFORD UNIVERSITY PRESS
Slides in PDF format of all the lectures will be provided by the teacher.
The teacher receives the students at least once a week by appointment via e-mail: tiziana.persichini@uniroma3.it
|
6
|
BIO/06
|
32
|
20
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410491 -
Fisiologia della risposta cellulare allo stress
(objectives)
The primary objective of the course is to enlarge and update student’s knowledge about the physiology and regulation of cellular functions mainly regarding the molecular mechanisms activated by cells in response to stressing conditions to maintain intracellular homeostasis.
-
FIOCCHETTI MARCO
( syllabus)
Introduction to the molecular mechanisms of cellular functions regulation: membrane and nuclear receptors, mechanisms of intracellular signal transduction and amplification through protein’s post-translational modifications (e.g. phosphorylation).
Definition of cellular stress and physiological tolerance range. Description of intracellular mechanisms, at DNA, RNA and protein levels, through which cells sense and respond to a broad variety of stressing conditions, focusing the attention mainly on physiological response and cell adaptation to extracellular stressing stimuli, including heat, osmotic, hypoxic, oxidative and nutrient stresses.
Evaluation of intracellular organelles- specific stress response, including mitochondria- and ER-stress response.
Description of down-stream molecular mechanisms activated by cells in response to stress and devote to re-establish the intracellular homeostasis and to adapt themselves to the changed conditions. Brief description of the abnormal stress response and adaptation in pathological cell degeneration (e.g. cancer).
Definition and update of the state-of-art about the laboratory research approaches applied to the study of the physiological stress response and adaptation and the molecular mechanisms behind these functions.
( reference books)
The class will be provided with the study materials found by students and by the professor.
|
6
|
BIO/09
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410493 -
Psicobiologia
(objectives)
The course will address the main topics of interest in psychobiology, with the aim of promoting a basic knowledge of the subject, of the main experimental paradigms and methods used in psychobiology.
The aim of the course is therefore to provide knowledge of the biological and neuropsychological fundamentals and correlates of behavior and psychological functions in humans and animals, as well as the main methods and techniques used in psychobiological research.
|
6
|
BIO/14
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410496 -
Laboratorio di neuroscienze
|
|
|
20401671 -
GENERAL PATHOLOGY
(objectives)
The aims of the course are:
1) to know the aetiology and the molecular mechanisms of the human diseases;
2)to know how to interpret the pathophysiological mechanisms of the human diseases.
|
6
|
MED/04
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410495 -
Microbiomica
(objectives)
The goal of the Microbiomics course is to provide information about the structure, dynamics, evolution, and methods to investigate complex microbial communities as a whole. The rapid progress of omic sciences (genomics, transcriptomics, proteomics and metabolomics) combined with high-throughput genome sequencing, global gene expression analysis tools, and powerful analytical method of metabolites has made feasible the analysis of complex and diverse microbial communities and their products as a whole.
The course is intended to provide state-of-art information on the impact of the microbiota in agriculture, food production, as well as animal and human health
-
VISCA PAOLO
( syllabus)
BASIC TOPICS: Introduction to the genomics of prokaryotes and its study techniques (sequencing, annotation, gene family content, duplications and deletions); Horizontal gene transfer and mobilome; metagenomics; Transcriptomics and gene chips; Proteome and interactome; metabolomics; Genomics of single cells; Systems biology and health; Genetic manipulations - Expression of heterologous genes in bacteria - Evolution of bacterial genomes; Molecular phylogeny; Microbial taxonomy methods and species concept; Classification of prokaryotes; Culture analysis of microbial communities; Enrichment cultures; Isolation of single cells; Laser forceps; Flow cytometry; Microfluidic techniques; High-throughput systems; Staining methods; Fluorescent in-situ hybridization (FISH); Gene amplification methods for the analysis of microbial communities; Microarray analysis of the genetic and functional diversity of microorganisms; Environmental genomics and study methods; Measurement of microbial activity in nature; Examples of symbiosis: lichens, intestinal polymicrobial systems (rumen); General information on the huiman microbiome; Gastrointestinal microbiota; Oral and airway microbiota; Microbiota of the urogenital tract and skin; Development and evolution of the human microbiota; Studies on humans and in animal models; Colonization, microbial sequences and stability of the intestinal microbiota; Diseases associated with intestinal dysbiosis; Diseases associated with dysbiosis of the oral cavity, skin and genital tract; Antibiotics, resistome and induced alterations of the microbiome; Probiotics and prebiotics; Microbiota and intestine-brain axis.
APPLICATIONS: Technologies for sequencing and NGS; Culturome and chemotaxonomy; Sequencing data preparation and analysis; Phylogeny based on 16S rRNA, 25-28S rRNA, ITS etc .; Tools to analyze the structure and dynamics of the microbiota: Whole Genome Sequencing (WGS) and metagenomics shotgun; Analytical databases, tools and pipelines (annotations); Resistome; Virulome; Virome.
IN-DEPTH: illustration of monothematic articles on the microbiota of humans, animals and plants, in relation to physio-pathological alterations. Groundbreaking articles and reviews will be selected from scientific journals year by year.
( reference books)
"Brock biology of microorganisms" (English) by Michael T. Madigan (Author), Kelly S. Bender (Author), Daniel H. Buckley (Author), W. Matthew Sattley (Author), David A. Stahl (Author): chapters 9.1-9.14; 12.1-12.5; 12.9; 12.11-12.13; 13.5-13.10; 19.1-19.12; 23.1; 23.12, 23.13; 24.1-24.11
Microbiota in health and disease: from pregnancy to childhood. Editors Pamela D. Browne, Eric Claassen and Michael D. Cabana Published: 2017 Pages: 344. eISBN: 978-90-8686-839-1 | ISBN: 978-90-8686-294-8 https://doi.org/10.3920/978-90-8686-839-1
The human microbiota: how microbial communities affect health and disease / edited by David N. Fredricks.
ISBN 978-0-470-47989-6
|
6
|
BIO/19
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20401777 -
PUBLIC HEALTH
(objectives)
General objectives:
at the end of the course, the student will be able to design epidemiological studies to address public health problems and to identify activities to prevent chronic and infectious diseases.
Specific objectives:
• know the major mortality and morbidity indicators
• to identify the epidemiological study more appropriate to describe the burden of major diseases in the population and to assess the relation between diseases and possible risk associated to them
•to interpret epidemiological data
•to discuss the importance of the health education and of the health promotion
•to list and discuss problems related to water supply, sanitation and waste management
•to argue about necessary controls/monitoring to protect the environment and the community
•to design health promotion programmes.
|
6
|
MED/42
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410259 -
ENTOMOLOGIA
(objectives)
The main formative targets are:
1) the acquisition of a basic knowledge on morphology, anatomy, physiology and evolution of the main insect groups;
2) the acquisition of both theoretical and practical knowledge on systematics and classification of the hexapoda;
3) the construction of a solid cultural background for an advanced discussion on causes and effects of the amazing evolutionary success and diversity of insects and on their importance in basic and applied studies;
4) the acquisition of the tools for the insect taxonomic identification, expecially for those orders and families present in the italian fauna;
5) to stimulate the curiosity and the ability of critically observe the nature;
6) the self-evaluation by the student of the competence whitin the animal biology, and in particular in the entomology.
|
6
|
BIO/05
|
32
|
20
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410295 -
ETNOBOTANICA ED ETNOZOOLOGIA
(objectives)
The relationship between humans and the environment: ethnobotany to ethnoecology.
Methods used in ethnobiology. Uses of wild plants (medicinal, food, handicrafts, cosmetics).
Ethnobotany and its possible applications (e.g., education, urban home-gardens, tourism, food sector, herbalism, pharmacognosy).
Ethnobiology in various geographical and cultural contexts and relationships with anthropology.
The contribution of ethnozoology to zoology and biogeography. Folk taxonomy and its relationship with the scientific taxonomy.
Traditional and Local Ecological Knowledge.
Overview of subsistence strategies of indigenous communities (hunter-gatherers, farmers, pastoralists).
The role of ethnobiology in environmental conservation and restoration.
Concepts of adaptation, resilience, vulnerability and tipping points in Social-Ecological Systems.
An analysis of the tragedy of the commons in social-ecological systems (e.g., unregulated fishing and hunting, threatened species).
Traditional management systems of Common resource systems.
Ethnobiology and Ecosystem Services
-
Derived from
20410295 ETNOBOTANICA ED ETNOZOOLOGIA in Biodiversità e gestione degli ecosistemi LM-6 SAVO VALENTINA
( syllabus)
The relationship between humans and the environment: ethnobotany to ethnoecology. Methods used in ethnobiology. Uses of wild plants (medicinal, food, handicrafts, cosmetics). Ethnobotany and its possible applications (e.g., education, urban home-gardens, tourism, food sector, herbalism, pharmacognosy). Ethnobiology in various geographical and cultural contexts and relationships with anthropology. The contribution of ethnozoology to zoology and biogeography. Folk taxonomy and its relationship with the scientific taxonomy. Traditional and Local Ecological Knowledge. Overview of subsistence strategies of indigenous communities (hunter-gatherers, farmers, pastoralists). The role of ethnobiology in environmental conservation and restoration. Concepts of adaptation, resilience, vulnerability and tipping points in Social-Ecological Systems. An analysis of the tragedy of the commons in social-ecological systems (e.g., unregulated fishing and hunting, threatened species). Traditional management systems of Common resource systems. Ethnobiology and Ecosystem Services.
( reference books)
Caneva G., Pieroni A., Guarrera P.M. (eds) 2013. Etnobotanica: Conservazione di un patrimonio culturale immateriale come risorsa per uno sviluppo sostenibile nel bacino del Mediterraneo. Edipuglia Bari.
Office Hours: every day of the week by appointment by email: valentina.savo@uniroma3.it
|
| 4
|
BIO/03
|
32
|
-
|
-
|
-
|
Attività formative affini ed integrative
|
| 2
|
BIO/05
|
20
|
-
|
-
|
-
|
Attività formative affini ed integrative
|
|
ITA |
|
20401821 -
IMMUNOLOGY
(objectives)
Cellular and molecular mechanisms involved in the immune response with a particular interest for those implicated in the response against pathogens.
-
Coccia Eliana Marina
( syllabus)
Properties and Overview of Immune Responses: Cells and Tissues of the Immune System
Leukocyte Circulation and Migration into Tissues
Innate Immunity
Antigens and Antigen presentation
Immune Receptors and Signal Transduction
Lymphocyte T Development and Activation
Differentiation and Functions of CD4+ and CD8 +Effector T Cells
B Cell Activation and Antibody Production
Humoral Immunity
Immunologic Tolerance and Autoimmunity
Immunity to Microbes and Vaccines
Immunodeficiency and immunosenescence
Immunological techniques
SEMINAR:
PAMPS AND TLRS
DENDRITIC CELLS
MICRO-RNA
IMMUNOPHAGY
BIBLIOGRAPHIC RESARCH - PUBMED
( reference books)
- Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai
IMMUNOLOGIA CELLULARE E MOLECOLARE
(8th Edition, Edizioni Edra)
- Peter Parham
IL SISTEMA IMMUNITARIO
(2nd Edition, Edizioni EdiSES)
|
6
|
MED/04
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410325 -
METODI E TECNICHE DI EDUCAZIONE E DIVULGAZIONE NATURALISTICA ED AMBIENTALE
(objectives)
To grow in the students knowledge, skills and skills professionalized in the fields of education and naturalistic and environmental dissemination.
Getting to know the students the professions related to dissemination and education in natural and environmental field, potential working unleash for specialists in the sector of management and the protection of ecosystems, information and scientific dissemination, and teaching in the field of natural.
|
6
|
BIO/07
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20402204 -
BIO-ORGANIC CHEMISTRY
(objectives)
The course will address the chemical transformations shared by organic chemistry and biological systems. Indeed, although the functional group elaborations are formally the same, the followed reaction pathways result to be quite different with respect to the experimental conditions of both organic syntheses and biological processes.
In such a context, the enzymatic action modes in living systems will be compared with the key role performed by temperature, pressure, ph, catalysts and solvents.
Keeping this in mind, a few of the most well-known chemical reaction mechanisms will be studied, underlining, step by step, the main similarities and distinctions with the analogous biological transformations.
-
GASPERI TECLA
( syllabus)
Cultural skills (Knowledge of): The course aims to provide students with the chemical basis of the structure, the properties, and the reactivity of biological molecules, as well as the main notions for understanding the logic that regulates bio-organic chemistry processes.
Methodological skills (How to carry out): at the end of the course, the student will be able to analyze and discuss the catalytic mechanism of the most important classes of enzymes, as well as to understand the importance of the organic chemistry applied to the study of biological systems, to biocatalysis, and to the development of biologically active molecules.
Introduction to Bio-organic Chemistry (A short overview of the most common functional groups involved in biological processes, highlighting their main features).
Organic chemistry mechanisms to explain key steps in pivotal biological pathways:
• Electrophilic Addition Reactions:
regioselective epoxidation of alkenes; the action of squalene epoxidase in squalene/lanosterol transformation.
• Nucleophilic Substitution Reactions
bimolecular Nucleophilic Substitution Reaction (SN2): the S-adenosylmethionine (SAM) role in the methyltransferase-catalyzed reactions;
carbocation chemistry in the nucleophilic substitution reaction (SN1): the IPP isomerase.
• Elimination reactions:
β-elimination reaction and the enolase role in the synthesis of the phosphoenolpyruvate (PEP).
• Nucleophilic Carbonyl Addition Reactions
imine and enamine formation; how Schiff bases act in the PLP-dependent enzymatic catalysis;
acetals and sucrose synthase;
Michael additions and the histidine ammonia-lyase function.
• Isomerization and epimerization:
keto-enol tautomerism and ribose-5-phosphate isomerase catalysis;
Inversion and retention of the stereochemical configuration: the glucosidase mechanism.
• Carbonyl condensation reactions:
aldol condensation promoted by aldolases (classes I and II)
Claisen condensation and the acetyl synthase function in the fatty acids synthesis.
• Acyl Nucleophilic Substitutions:
the hydrolysis of esters and the mechanism of Human Pancreatic Lipase;
esterification reactions and the synthesis of triacylglycerols by means of acyl-CoA synthetase and acyltransferase;
amide bond formation; asparagine synthetase vs glutamine synthetase;
the hydrolysis of amides and the chymotrypsin action mode.
• Oxidations and reductions
metal hydride and the reduction of the ketone carbonyl group in acetoacetyl ACP due to the β-keto thioester reductase;
Baeyer-Villinger oxidation and the hydroxyacetophenone monooxygenase;
ozonolysis reactions and dioxygenase enzyme activity.
• Carboxylation reactions:
Grignard reactions in CO2 atmosphere; mechanisms of both the pyruvate carboxylase and Ribulose-1,5-bisphosphate carboxylase oxygenase (RuBiscO);
the decarboxylation reaction in both malonic and acetoacetic synthesis; the key role of thiamine pyrophosphate (TPP) in the 1-deoxy-D-xylulose 5-phosphate synthase catalysis.
• Noteworthy examples: pyruvate dehydrogenase complex, the kynurenine catalysis and the tryptophan metabolism; anomalous features in histidine metabolism.
( reference books)
John McMurry, Tadhg Begley in “Chimica Bio-Organica”, Zanichelli Ed. spa
T.W. Graham Solomons; Craig B. Fryhle in “Organic Chemistry”, 10th Edition, Wiley.
John McMurry in “Chimica Organica”, Piccin-Nuova Libreria
Bruno Botta in “Chimica Organica” Edi-ermes
Lecture notes and bibliographical references will be provided
The teacher receives Tuesday from 17.00 to 19.00 by appointment via e-mail: tecla.gasperi@uniroma3.it
|
6
|
CHIM/06
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410284 -
PARASSITOLOGIA EVOLUZIONISTICA
(objectives)
Offering the students a new perspective in the study of parasitology, by means of an ecological, evolutionary and comparative approach.
The intimate and intricate nature of the association between hosts and parasites, has traditionally been studied by giving emphasis on its pathogenicity for non-human and human animal hosts.
In contrast, this course offers the student the opportunity to deepen topics such as adaptive strategies of parasitic life-style, the impact of parasitism on the community structure or else the role of a parasite in the trophic energy transfer in the community.
Emphasis will be given to the host as key element in the parasite environment and, as such, its important role as a selective pressure.
|
6
|
BIO/05
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410321 -
VIROLOGIA ANIMALE GENERALE ED APPLICATA
(objectives)
Deepening the knowledge about animal viruses (origins, classification, replicative strategies, virulence, immunoevasion mechanisms) and their applications (antiviral vaccines, viral vectors for gene expression, oncolytic viruses).
-
AFFABRIS ELISABETTA
( syllabus)
The course describes and compares the replication cycles of different animal viruses that infect vertebrate hosts to gain insight into: origin of viruses, structure, evolution, virus-cell interactions, host defense mechanisms and strategies of viral immunoevasion, methods of detection, identification and titration, antiviral drugs and profilaxis of viral infections. In particular, it will be described viruses of the following viral families: Parvoviridae, Poliomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae, Picornaviridae, Flaviviridae, Coronaviridae, Rabdoviridae, Orthomixoviridae, Paramixoviridae, Retroviridae, Hepadnaviridae. In addition, introduction to applied virology will be provided (i.e., vaccine development, viral vectors and oncolytic viruses). Students will be able to apply the acquired knowledge to understand in-depth texts and experimental data related to the discipline.
( reference books)
All the detailed list of topics covered during the lessons, the PDF files of power point presentations used during the course and further materials are available in the Moodle platform of the course.
Adopted text book:
- N. J. Dimmock, A. J. Easton, K. N. Leppard - Introduction to Modern Virology - Seventh edition 2016 -Wiley Blackwell - ISBN 978-1-119-97810-7
- Leonard Norkin – Virology: Molecular Biology and Pathogenesis - ASM (American Society for Microbiology) Press (this text book is more detailed)
to complement the PDF files of the power point used by the teacher during the course.
Mandatory integrations of the PDF files regarding viral vectors (adenoviral, adeno-associated, retroviral and lentiviral vectors, poxvirus-based vectors) and oncolitic viruses (general introduction + oncolytic adenoviruses) can be found herebelow:
- Fields virology, Vth edition, 2007- D.M.Knipe and P.M.Howley editors-in-chief -Lippincot Williams & Wilkins publishers, cap.16 Viral vectors and their applications (on line access through Roma Tre Scientific Library)
- Stéphanie Durand and Andrea Cimarelli - The Inside Out of Lentiviral Vectors - Viruses 2011, 3, 132-159.
- Carina Elsner and Jens Bohne - The retroviral vector family: something for everyone. – Virus Genes 2017, 53:714-722
The teacher receives by appointment via email (elisabetta.affabris@uniroma3.it) or at the end of the lessons.
|
6
|
BIO/19
|
40
|
-
|
10
|
-
|
Related or supplementary learning activities
|
ITA |
|
20402025 -
Bioindication and Environmental Monitoring
(objectives)
Knowing the importance of the bioindication and the use of plants and animals as bioindicators for monitoring environmental quality status (water, air, soil).
Have a thorough knowledge of the instrumental methods, acquisition methodology, data analysis in the field of bioindication and ecosystem monitoring.
Acquire the knowledge for using the modern systems of bioindication, biomonitoring and bioremediation.
-
Derived from
20402025 BIOINDICAZIONE E MONITORAGGIO AMBIENTALE in Biodiversità e gestione degli ecosistemi LM-6 CESCHIN SIMONA
( syllabus)
Bioindication: basic concepts. Main ecological characteristics of a Bioindicator. Two ways for bioindicating: react and bioaccumulate. The homeostasis of the Bioindicators. Time and ecological relationship of the bioindicators' responses to environmental variations (perturbations). What are a stress and a stressor.
Bioindication in different levels of biological organization. Integrity and funzionality of the Ecosystem. Status verification of the biocoenoses and evaluation of the environmental quality. Two different approaches: the theories of the Top-Down and of Buttom-Up.
Bioindication and environmental monitoring. Bioindication in aquatic, edaphic and atmospheric habitat. Examples of application of the bioindication techniques. Evaluation of the environmental quality by ecological and biotic indices. Standard and sperimental techniques for monitoring activities. The bioindication in Italy. Main European and Italian directives. International and national Agencies, Authorities, Institutions for environmental monitoring.
( reference books)
Pdfs of the lessons performed during the course and the book: Bioindicatori ambientali, 1998, edited by F. Sartori, Graphic Arts Juri Iodice, Sannazzaro (PV).
The teacher receives Mon, Wed, Fri from 9.00 to 10.00 by appointment via email: simona.ceschin@uniroma3.it
|
6
|
BIO/02
|
40
|
10
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20402508 -
NATURAL PRODUCTS AND ENVIRONMENTAL CHEMISTRY
(objectives)
The course aims at giving:
1) a general knowledge of the biosynthetic pathways of secondary metabolism;
2) information about the main natural products derived from microorganism, plants and animals and their role in natural environment;
3) knowledge on the structure/biological activity relationship of the main secondary metabolites used in pharmaceutical and healthcare industry.
4) attainment of the basic educational background necessary to understand the chemical equilibria that regulate the origins, the transport, the transformations and the effects of the environmental chemical species, either of natural or human origin.
-
Derived from
20402508 CHIMICA DELLE SOSTANZE NATURALI E DELL'AMBIENTE in Biodiversità e gestione degli ecosistemi LM-6 N0 TOFANI DANIELA
( syllabus)
-Introductory notes on the chemistry of natural substances. Characteristics of secondary metabolites. Allelopatic substances. The biosintetic ways of secondary metabolism.
Metabolism of acetate. Prostaglandins. Aromatic polychetides. Anthraquinones. Cannabinoids. Aflatoxins. Tetracycline.
The metabolism of shikimato. Biogenesis of shikimato. Cinnamic acids. Lignins. Fenilpropani. Benzoic acids. Coumarins. Flavonoids. Tannins. Isoflavones. Terpenoid chinons.
The metabolism of the mevalonate. Biogenesis of mevalonic acid. The various types of terpens and derived compounds. Iridoids. Gibberellins. Structural characteristics of animal steroids. Phytosterols.
Alcaloids: definition, original amino acids and nomenclature. Biosynthesis and activities of the main types of alcaloids: cocaine, nicotine, curari, morphine, loganin, lysergic acid, coniine.
Notes on carbohydrates. Cianogenic glucosides. Streptomycin.
Secondary metabolites of peptidic origin. Interferons. Opious peptides. Peptidic toxins: ricina and botulino. Penicillins.
Basic principles of environmental chemistry. Interactions between the various spheres in which the terrestrial environment is divided.
The atmosphere: notes on training and depositing of the ozone (buco dell'ozono); effect of cfc and halon. Notes on oxidizing and polluting species and their monitoring. The particulate: ipa and phthalates. The serra effect: main gas-greenhouse and their gwp. The kyoto protocol.
The hydrosphere. Chemical-physical properties of water and water bodies. TIC, TOC, TC. Biodegradable organic pollutants (COD, BOD5), of difficult oxidation and toxic (LD50 and LOD50).
Bioaccumulation and biomagnification. Metabolism of some pesticides.
( reference books)
P.M. DEWICK : "Medicinal Natural Products" Wiley Ed.
C. BAIRD, M. CANN “Environmantal Chemistry”,
|
6
|
CHIM/06
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410301 -
ECOLOGIA E GESTIONE DEGLI ECOSISTEMI COSTIERI
(objectives)
Major goals of this course are:
to acquire an appropriate knowledge of the biodiversity and of general principles of the ecology of coastal ecosystems and their relationship with other ecological branches (plant and animal ecology).
Other goal of this course is the growth of a thorough interest to ecological problems discussing the relationship between man and nature focusing in the coastal environment, analyzing main disturbance factors.
The course is also intended to develop basic insights for the acquisition (sampling and monitoring), organization and interpretation of ecological data recorded in coastal ecosystems.
|
| 4
|
BIO/03
|
16
|
20
|
-
|
-
|
Attività formative affini ed integrative
|
| 2
|
BIO/05
|
16
|
-
|
-
|
-
|
Attività formative affini ed integrative
|
|
ITA |
|
20410287 -
METODI E TECNICHE IN ECOLOGIA DELLA VEGETAZIONE
(objectives)
The acquisition of the knowledge on the main methods for the study of vegetation are assumed as formative objectives.
In particular:
-acquire the knowledge about main methods of vegetation study;
-increase the ability to interpret the natural and artificial phenomena with particular attention to the plant landscape mechanisms;
-develop the capacity of choice the most appropriate study and monitoring methodologies for conservation and management purposes;
-implement the ability of interpretation of experimental data and scientific articles in the fields of plant ecology, geobotany and applied ecology.
|
6
|
BIO/03
|
32
|
20
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20402518 -
TOXICOLOGY
(objectives)
The course aims to provide students with fundamental knowledge on the basic principles of toxicology. In particular, the toxicodynamic and toxicokinetic mechanisms that are behind the toxic effects produced by different natural and synthesized xenobiotics, and the process of evaluation of toxicological risks will be analyzed. This knowledge will allow students to acquire specific professional skills useful in experimental and analytical research laboratories and to carry out professional activities in the pharmaco-toxicological field to protect the safety of the environment, food, cosmetic products and drugs, of both synthetic and natural origin.
-
TREZZA VIVIANA
( syllabus)
GENERAL PRINCIPLES. AIMS AND FIELDS OF TOXICOLOGY. CLASSIFICATION OF TOXIC AGENTS. MECHANISMS OF MOLECULAR AND CELL TOXICITY. RISK EVALUATION. TOXICOKINETICS: ABSORPTION, DISTRIBUTION, METABOLISM AND EXCRETION OF TOXIC AGENTS. ADVERSE DRUG REACTIONS, DRUG INTERACTIONS AND PRINCIPLES OF PHARMACOVIGILANCE. ANTIDOTES AND THEIR CLASSIFICATION. EXPERIMENTAL EVALUATION OF TOXIC EFFECTS IN IN VITRO AND IN VIVO MODELS AND DATA EXTRAPOLATION. SYSTEM AND ORGAN TOXICOLOGY. DEVELOPMENTAL AND FEEDING TOXICOLOGY. CLASSES OF TOXIC COMPOUNDS: METALS, PHYTODRUGS, NATURAL TOXINS. PSYCHOACTIVE AND DOPING DRUGS.
( reference books)
CASARETT & DOULL'S “ESSENTIALS OF TOXICOLOGY”, SECOND EDITION, MCGRAW HILL
Power-point files of the lessons will be provided. The files are also available on the MOODLE platform
To get an appointment with the Professor, email to: viviana.trezza@uniroma3.it
|
6
|
BIO/14
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410253 -
NEUROBIOLOGIA CELLULARE APPLICATA
(objectives)
The aims of this course are: to gain knowledge of the specialized cellular architecture of glia and neurons; to deepen the knowledge of cellular and molecular mechanisms involved in cell communication between glia and neurons. Special emphasis will be given to nitric oxide-regulated pathways. in this course students can use state of the art equipment and learn current techniques employed in cell biology research labs. moreover students are allowed to critically discuss the scientific literature in the field of neurobiology.
|
6
|
BIO/06
|
32
|
-
|
20
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410213 -
Nutrienti di origine vegetale
(objectives)
The main objective of the course is to provide basic knowledge on the main nutrients present in different food plants, such as carbohydrates, lipids and proteins. Particular attention is paid to the biochemical plasticity of the secondary metabolism which leads to the formation of both secondary metabolites and bioactive compounds with beneficial effects on human health, and of anti-nutritional or toxic factors.
-
CONA ALESSANDRA
( syllabus)
Origin of agriculture and plant domestication. Characteristics of the main crops: Poaceae, Fabaceae, Rosaceae, Solanaceae, Brassicaceae. Nutritional values of the different plant organs and nutrients bioavailability. Primary and secondary metabolism. Nutrients from plants: proteins, lipids and oils, carbohydrates, fibers, minerals, vitamins and antioxidants. Nutritional values of secondary metabolism and bioactive compounds: terpenes, phenols, alkaloids, cyanogenic glucosides, glucosinolates, non-protein amino acids. Effects of the environmental conditions on primary and secondary metabolism: food quality and production of aromas, flavors and toxins. Plant bioactive compounds as dietary supplements. Effects of storage conditions and pre- and post-harvest diseases on nutritional quality of plant foods. Allergy and intolerances caused by plant substances present in crop species. Genetic improvement and biotechnology approaches to develop crop plants with high nutritive contents and resistant to environmental stress.
( reference books)
1. Piante alimentari. Biologia, composizione chimica, utilizzazione. Rinallo C.; Piccin.
2. Biologia delle Piante. Vol 2: Interazioni con l’ambiente e Domesticazione. Smith A. M. et al.; Zanichelli.
3. Lecture notes and scientific articles provided by the teacher
The professor receives every day by appointment via e-mail: alessandra.cona@uniroma3.it
|
6
|
BIO/04
|
44
|
5
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410214 -
Nutrigenomica
(objectives)
The course will deal with diet-genome interactions and the application of nutritional strategies in health maintenance.
Students should become familiar with the concept that external molecules present in our foods can affect human metabolism and gene expression in different cells and tissues. The course will address the utilization of the concept of “system biology” to nutrition
-
VIRGILI FABIO
( syllabus)
Role of nutrients and bioactive dietary molecules in the regulation of gene expression; Application of "omics" methodologies in the study of system nutrition; effects of diet-genotype interaction on the individual response to dietary stimuli; Nutritional phenotype; elements of Bayesian statistics
( reference books)
Principles of Nutrigenetics and Nutrigenomics. Academic Press De Caterina, Martinez & Kohlmeier Eds
either (in italian)
Nutrigenomica ed epigenetica. Edra Ed. Galimberti et al Eds
A selection of scientific papers provided by the teacher
|
6
|
BIO/11
|
48
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410273 -
NEUROBIOLOGIA DELLO SVILUPPO
(objectives)
Aim of the course is to provide students with advanced knowledge of developmental biology, focussing on vertebrate nervous system.
Cellular and molecular mechanisms underlying neural differentiation and morphogenesis will be addressed in different in vivo and in vitro models.
The role of neurogenesis and plasticity throughout the lifespan of individuals will be examined.
Students will be able to:
1) describe major neural processes in vertebrates;
2) identify molecular pathways driving stem cells to neuronal or glial cell fates;
3) correlate abnormal cellular processes with specific neurodevelopmental or age-related disorders.
-
MORENO SANDRA
( syllabus)
Overview of developmental processes of pluricellular organisms. Germ cell layers. Ectodermal derivation of neural tissue in invertebrates e vertebrates. Specification of neuroectoderm and neurulation process in vertebrates. Primary neurulation: cellular e molecular mechanisms of neural plate, folds, furrow, and tube formation. Secondary neurulation. Neural tube defects in mammals: genetic and environmental causes of anencephaly and spina bifida. Morphogenesis and differentiation of the encephalon and spinal cord. Role of cell death in the morphogenesis of the central nervous system: apoptotic and autophagic pathways. Neural crest: specification and regionalization. Cell migration and differentiation of cephalic, cardiac, trunk, vagal e sacral neural crest. Multipotency e plasticity of neural crest cells. Layering of cortical structures in the cerebrum and cerebellum. Congenital diseseases associated with defects to neuronal migration: the case of peroxisomal disorders. Neural stem cells: neuronal and glial differentiation. Adult neurogenesis in mammals: the subventricular and subgranular zones. The concept of stem cell niche. Regulatory pathways of the neurogenic niche. Neurogenesis and neurodegeneration in the senescent brain. Age-related neurodegenerative diseases: common and specific features. Protein aggregates, role of apoptotic and autophagic pathways, and stem cell-based therapies against neurodegeneration.
( reference books)
Gilbert: "Developmental Biology", Tenth edition, Sinauer Associates, Inc., 2014
Sanes et al.: "Development of the nervous system", Elsevier Inc., 2012
|
6
|
BIO/06
|
40
|
-
|
10
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410319 -
FISIOLOGIA CELLULARE E MOLECOLARE
(objectives)
The course aims to deliver knowledge on some aspects of the regulation of cellular function on the basis of the most recent published works.
|
6
|
BIO/09
|
40
|
-
|
10
|
-
|
Related or supplementary learning activities
|
ITA |
|
20410305 -
LABORATORIO DI MICROSCOPIA ELETTRONICA
(objectives)
The course aims to provide the student with theoretical knowledge and technical skills to adress the ultrastructural morphological study of biological materials.
The educational objectives contain:
1) learning of the basic principles of electronic microscopy;
2) knowledge and application of methodologies related to the preparation of biological samples of different nature (organisms procarioti and eucarioti) for ultrastructural analysis;
3) use of ultrastructural survey tools (scan, transmission and ionic beam microscopes);
4) observation, capture and elaboration of microscopic images;
5) qualitative interpretation and quantitative analysis of ultrastructural data.
The student will be able to autonomously prepare an experimental protocol appropriate to the type of sample and the investigation objective.
The knowledge acquired during the course will also allow the student to make a critical analysis of the morphological results in a functional context
|
| 3
|
BIO/05
|
8
|
-
|
20
|
-
|
Attività formative affini ed integrative
|
| 3
|
BIO/06
|
8
|
-
|
20
|
-
|
Attività formative affini ed integrative
|
|
ITA |
|
20410270 -
LABORATORIO DI BIOTECNOLOGIE DEI MICRORGANISMI
(objectives)
The main aims of the course are:
a) acquisition of knowledge on advanced methodologies in microbial biotechnology;
b) acquisition of appropriate metodologies and languages for the isolation, observation and description of industrial microorganisms;
c) acquisition of basic knowledge for drafting and executing experimental protocols.
-
RAMPIONI GIORDANO
( syllabus)
The course aims at providing to the student:
- adequate knowledge and understanding on advanced methodologies in microbial biotechnology, appropriate methodologies and communication skills for the isolation, observation, description and engineering of industrial microorganisms;
- adequate applied knowledge for drafting and executing experimental protocols, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).
Programme
During the laboratory practices and the corresponding theoretic lessons, the following topics will be covered:
- isolation of microorganisms of industrial relevance from the environment, preparation of growth media and cultivation techniques;
- transposon–based mutagenesis (generation and screening of mutant libraries);
- genetic transfer processes (conjugation and transformation);
- use of whole-cell bacterial biosensors for gauging specific molecules produced by other microorganisms.
The following topics will be covered in additional theoretic lessons:
- development of innovative therapeutic strategies based on biotechnological processes;
- development of biosensors and reporter systems for the identification of new drugs;
- application of synthetic biology to microbial biotechnology;
- properties of the regulatory networks and their application to biotechnological processes.
( reference books)
Selected topics will be available on suggested Microbiology and Microbial Biotechnology books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices.
Innovative teaching will be promoted by cloud-sharing of power-point presentations illustrating the main topics of the course. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.
Students will be received every Monday, from 3:00 to 4:00 pm. Students can be received at the best of their convenience if an appointment has been fixed at the end of the lessons or by email.
|
6
|
CHIM/11
|
53
|
-
|
-
|
-
|
Related or supplementary learning activities
|
ITA |
|
Università Roma Tre